Article
Microbiology
Junying Zhu, Xuemei Jiang, Lina Zhao, Min Li
Summary: An outbreak caused by carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) ST859-K19 isolates occurred in a teaching hospital in China. The isolates showed high-level resistance to commonly used antibiotics and had almost identical resistance and virulence gene profiles, indicating possible clone transmission. This study provides important insights into the molecular and genomic characteristics of ST859 CR-hvKP isolates and highlights the need for active surveillance to detect the spread of CR-hvKP in healthcare settings.
Article
Immunology
Dakang Hu, Wenjie Chen, Qi Zhang, Meng Li, Zehua Yang, Yong Wang, Yunkun Huang, Gang Li, Dongxing Tian, Pan Fu, Weiwen Wang, Ping Ren, Qing Mu, Lianhua Yu, Xiaofei Jiang
Summary: This study investigated the epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) and hypervirulent carbapenem-resistant Klebsiella pneumoniae (Hv-CRKP) in mainland China. Molecular determination of CR-HvKP and Hv-CRKP showed biases compared to mouse lethality test, with the exact prevalence of CR-HvKP being less than 1.0% and that of Hv-CRKP even lower.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Veronica Franca Diniz Rocha, Matheus Sales Barbosa, Helena Ferreira Leal, Giulyana Evelyn Oliveira Silva, Nabila Monalisa Mendes Dantas Sales, Adriano de Souza Santos Monteiro, Jailton Azevedo, Allan Roberto Xavier Malheiros, Ledilce Almeida Ataide, Beatriz Meurer Moreira, Mitermayer Galvao Reis, Fabianna Marcia Maranhao Bahia, Joice Neves Reis
Summary: This study investigated the genetic relationship, antimicrobial susceptibility profile, and resistance mechanisms to ColR-CRKP isolates from patients infected/colonized in a tertiary hospital in Salvador, Bahia/Brazil. The study found a prolonged outbreak of ColR-CRKP in the region, suggesting possible cross-transmission.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Courtney L. Luterbach, Liang Chen, Lauren Komarow, Belinda Ostrowsky, Keith S. Kaye, Blake Hanson, Cesar A. Arias, Samit Desai, Jason C. Gallagher, Elizabeth Novick, Stephen Pagkalinawan, Ebbing Lautenbach, Glenn Wortmann, Robert C. Kalayjian, Brandon Eilertson, John J. Farrell, Todd McCarty, Carol Hill, Vance G. Fowler, Barry N. Kreiswirth, Robert A. Bonomo, David van Duin
Summary: This study evaluated the clustering of carbapenem-resistant Klebsiella pneumoniae (CRKp) among patients in US hospitals and identified widespread intrasystem and intersystem transmission. Different methods for assessing genetic similarity resulted in only minor differences in interpretation.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Irina Magdalena Dumitru, Mirela Dumitrascu, Nicoleta Dorina Vlad, Roxana Carmen Cernat, Carmen Ilie-Serban, Aurelia Hangan, Raluca Elena Slujitoru, Aura Gherghina, Corina Mitroi-Maxim, Licdan Curtali, Dalia Sorina Carp, Anca Dumitrescu, Romelia Mitan, Rodica Lesanu, Sorin Rugina
Summary: Infections with carbapenem-resistant Enterobacteriaceae pose a significant challenge in healthcare settings, especially with CRKP being resistant to the majority of available antimicrobial agents and leading to high rates of morbidity and mortality. A recent report of patients in an ICU with COVID-19 who developed invasive infections due to CP-Kp strains highlights the importance of rigorous antibiotic administration programs in intensive care units, especially for high-risk populations like the elderly.
Article
Microbiology
Feiyang Zhang, Qin Li, Jiawei Bai, Manlin Ding, Xiangjin Yan, Guangxi Wang, Baoli Zhu, Yingshun Zhou
Summary: This study revealed that 8.39% of Klebsiella pneumoniae strains were amikacin-heteroresistant, with potential association with increased expression of aminoglycoside resistance genes. The majority of heteroresistant phenotypes were unstable, with partial or full reversion of minimal inhibitory concentrations to the level of susceptibility.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Gabriel Mendes, Maria Leonor Santos, Joao F. Ramalho, Aida Duarte, Catia Caneiras
Summary: Hypervirulent and carbapenem-resistant pathotypes of Klebsiella pneumoniae have emerged as distinct evolutionary types with significant impact on public health. However, there is concern over the convergence of high-risk clones that pose a challenge to available therapeutic options. This review aims to describe the worldwide distribution of virulence factors among carbapenem-resistant highly virulent or hypervirulent K. pneumoniae strains and their interactions with carbapenemase production and sequence types. Urgent understanding of these strains is necessary for effective response as they increasingly replace classical strains in healthcare settings.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Xuemei Yang, Qiaoling Sun, Jiaping Li, Yu Jiang, Yi Li, Jianping Lin, Kaichao Chen, Edward Wai-Chi Chan, Rong Zhang, Sheng Chen
Summary: The epidemiological features and potential threat to human health of the newly emerged carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) are currently unknown. This study analyzed 784 CRKP strains collected from three hospitals in China and found that the proportion of CRKP strains among clinical K. pneumoniae strains increased sharply. A significant number of these CRKP strains carried a virulence-encoding plasmid, but only a small percentage exhibited a hypervirulent phenotype. Genetic markers were not strongly correlated with hypervirulent phenotypes, indicating that additional factors may contribute to the hypervirulence of CRKP. The transmission of these CRKP strains in China likely involved multiple clones of ST11 and the major mechanism of carbapenem resistance was the carriage of IncFII pSWU01-like, bla (KPC-2)-bearing plasmids. These findings highlight the rapid increase in prevalence of CRKP strains carrying virulence plasmids in China and the need for better definition and screening of truly hypervirulent CR-HvKP strains in clinical settings.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Biochemistry & Molecular Biology
Hao Guo, Yuye Wu, Lirong Li, Jianfeng Wang, Juan Xu, Fang He
Summary: The study investigated the global dissemination trends of carbapenem-resistant Klebsiella pneumoniae (CRKP) strains that co-carry multiple carbapenemases. A CRKP strain, KP424, co-carrying blaNDM-1 and blaKPC-2, was identified and sequenced. Genome sequences of CRKP strains carrying multiple carbapenemase genes were retrieved from the NCBI GenBank database. The prevalence of CRKP strains co-carrying two carbapenemase genes has significantly increased over time, with strains carrying both blaNDM and blaOXA-48-like being the most common worldwide. Further research is needed to understand the mechanisms of multiple carbapenemase gene occurrence and prevent their global dissemination.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Public, Environmental & Occupational Health
Na Huang, Huaiyu Jia, Beibei Zhou, Cui Zhou, Jianming Cao, Wenli Liao, Shixing Liu, Lingbo Wang, Liqiong Chen, Lijiang Chen, Tieli Zhou, Jianzhong Ye
Summary: This study investigated the clinical and microbiological characteristics of Klebsiella pneumoniae meningitis, revealing an association between antimicrobial resistance, virulence, and patient prognosis. The high fatality of meningitis caused by hypervirulent carbapenem-resistant K. pneumoniae strains is particularly concerning.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Immunology
Lingyi Zeng, Chengru Yang, Jisheng Zhang, Kewang Hu, Jingbo Zou, Jie Li, Jianmin Wang, Wan Huang, Lining Yin, Xiaoli Zhang
Summary: This study investigated the molecular characteristics and epidemiology of CRKP isolated from the ICU of a tertiary hospital in China. The results showed that the CRKP strains were highly resistant to various antibiotics and carried virulence genes. Clonal transmission of ST11 CRKP was observed in the ICU.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Jihui Chen, Yu Yang, Huimin Yao, Shuhong Bu, Lixia Li, Fang Wang, Feng Chen, Huijuan Yao
Summary: This study analyzed the clinical information of adult patients with carbapenem-resistant Klebsiella pneumoniae (CRKP) infection, established a nomogram for predicting mortality risk, and determined the effectiveness of different antimicrobial regimens. The results can help clinicians identify high-risk adult patients with CRKP infection, improve treatment outcomes, and reduce mortality.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Li Li, Renying Wang, Dan Qiao, Min Zhou, Peipei Jin
Summary: This study aimed to identify the origin and transmission route of a carbapenem-resistant Klebsiella pneumoniae (CRKP) outbreak in an emergency intensive care unit (EICU) and provide prevention and control strategies. The integration of genomic and epidemiological data yielded a clear transmission map of the CRKP outbreak. Early monitoring of the rapid evolution of CRKP, monitoring of CRKP after patient discharge, environmental hygiene, and effective antibiotic treatment are key to preventing and controlling CRKP outbreaks.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Immunology
Luana Soares de Moraes, Gerusa Luciana Gomes Magalhaes, Joao Gabriel Material Soncini, Marsileni Pelisson, Marcia Regina Eches Perugini, Eliana Carolina Vespero
Summary: This study evaluated clinical data from 107 patients with bloodstream infection caused by Klebsiella pneumoniae and conducted phenotypic and molecular analyses on 50.5% (54/107) of samples that exhibited resistance to carbapenems. The bla(NDM) gene was present in 90.4% (49/54) of these samples, bla(KPC) gene was found in one sample, and 7.4% (4/54) of samples showed no carbapenemase gene. The study identified four main clones and found that 11 samples were not genetically related. The median age of the patients was 58 years old, with a majority of males (60.7%). Comparing patients with and without carbapenem resistance, several factors including ICU stay, renal failure, previous antimicrobial use, Charlson's comorbidity index, invasive procedures, and death showed statistically significant differences. Additionally, renal failure, liver failure, and BSI with extensive-drug resistance (XDR) or pan-drug resistance (PDR) were predictors of increased mortality. The study concluded that patients with carbapenem-resistant pneumonia, regardless of additional resistance to polymyxins, experienced higher mortality rates.
MICROBIAL PATHOGENESIS
(2022)
Article
Infectious Diseases
Lijia Ni, Zhixian Zhang, Rui Shen, Xiaoqiang Liu, Xuexue Li, Baiji Chen, Xiquan Wu, Hongyu Li, Xiaoying Xie, Songyin Huang
Summary: Disinfectant resistance is a serious concern for hospital infection control due to the extensive use of disinfectants. Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a common and challenging pathogen in nosocomial infections. This study found that CRKP strains exhibited extensive resistance to various clinical disinfectants.
Article
Environmental Sciences
Margherita Napolitani, Giovanni Guarducci, Gulnara Abinova, Gabriele Messina, Nicola Nante
Summary: The aim of this study was to evaluate the concordance between health profiles outlined by ward doctors and epidemiologists and to study the impact of training interventions on the concordance. The results showed that after the training interventions, the concordance significantly improved, confirming the effectiveness of the training.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Public, Environmental & Occupational Health
R. Bosco, G. Cevenini, S. Gambelli, N. Nante, G. Messina
Summary: This study aimed to assess the impact of different disinfection levels and the use of a UVC device on microbial contamination in operating theatres. The results showed that the UVC device effectively improved environmental disinfection and standardized the level of environmental hygiene. There was no significant difference in microbial reduction between different disinfection levels.
JOURNAL OF HOSPITAL INFECTION
(2022)
Article
Environmental Sciences
Giovanni Guarducci, Gabriele Messina, Simona Carbone, Andrea Urbani, Nicola Nante
Summary: This study evaluates the provision of hospital orthopedic intensive rehabilitation and patients' migration patterns in Italy. The findings show that only six regions are able to meet the care needs of their residents, with positive epidemiological and financial balances.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Multidisciplinary Sciences
Davide Amodeo, Valentina Lucarelli, Isa De Palma, Alessandro Puccio, Nicola Nante, Gabriele Cevenini, Gabriele Messina
Summary: This study evaluated the efficacy of violet-blue light at 405 nm (VBL405) in inhibiting microbial growth on surfaces and in the air. The results showed that VBL405 had the strongest inhibitory effect on microbial growth, especially at a distance of 2 meters from the light source. Additionally, the average microbial reduction in the sampled air after exposure to VBL405 was approximately 70%.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Environmental
G. Messina, R. Bosco, D. Amodeo, N. Nante, I. De Palma, C. Petri, G. Cevenini
Summary: The effectiveness of near-UV LED ceiling lamps with a wavelength of 405 nm in improving environmental hygiene was evaluated in a kindergarten room. The study found that these lamps significantly reduced bacterial contamination in the air and on surfaces, highlighting their importance in controlling environmental contamination.
JOURNAL OF ENVIRONMENTAL HEALTH SCIENCE AND ENGINEERING
(2023)
Article
Health Care Sciences & Services
C. Salini, D. Amodeo, G. Cevenini, N. Nante, I. De Palma, G. Messina
Summary: This study aimed to evaluate the use of a nUV LED device at a wavelength of 405 nm on slush machines to reduce microbial contamination. The results showed a significant logarithmic reduction in microbial contamination with increasing distance from the nUV LED light source. The study concludes that nUV technology is effective in reducing the incidence of foodborne diseases.
ANNALI DI IGIENE MEDICINA PREVENTIVA E DI COMUNITA
(2023)
Article
Public, Environmental & Occupational Health
Eleonora Frilli, Davide Amodeo, Gabriele Cevenini, Nicola Nante, Gabriele Messina
Summary: This experimental study aimed to determine the activity of a near-UVA (405 nm) LED ceiling system against the SARS-CoV-2 virus. The ceiling system comprised 17 near-UVA LED lights with a radiant power of 1.1 W/each centred at 405 nm wavelength. The study found that the near-UVA LED ceiling system inhibited the replication of SARS-CoV-2 virus with a maximum measurable log reduction of 3.0 log(10) from a starting concentration of 10(7.2) TCID50/mL, suggesting its potential as an alternative treatment for localized infections and environmental decontamination.
EPIDEMIOLOGY AND INFECTION
(2023)
Article
Health Care Sciences & Services
Roberta Bosco, Gabriele Messina, Bruno Aiello, Giovanni Guarducci, Nicola Nante
Summary: This study aimed to investigate the structured activities of health promotion (HP) in Italy at national, regional, and territorial levels. The results showed great heterogeneity in HP activities in Italy, which may be attributed to limited attention to information digitalization.
Meeting Abstract
Public, Environmental & Occupational Health
B. M. Bocci, I. De Palma, D. Amodeo, G. Cevenini, N. Nante, G. Messina
EUROPEAN JOURNAL OF PUBLIC HEALTH
(2022)
Meeting Abstract
Public, Environmental & Occupational Health
E. Capitani, C. Lorenzini, E. Baragatti, L. Alaimo, G. Messina, N. Nante
EUROPEAN JOURNAL OF PUBLIC HEALTH
(2022)
Meeting Abstract
Public, Environmental & Occupational Health
A. Cartocci, V. Lucarelli, G. Messina, N. Nante, G. Cevenini, P. Barbini
EUROPEAN JOURNAL OF PUBLIC HEALTH
(2022)
Meeting Abstract
Public, Environmental & Occupational Health
V. Falcone, D. Romani, N. Nante, F. Cocchi, G. Messina
EUROPEAN JOURNAL OF PUBLIC HEALTH
(2022)
Meeting Abstract
Public, Environmental & Occupational Health
E. Frongillo, D. Amodeo, N. Nante, G. Cevenini, G. Messina
EUROPEAN JOURNAL OF PUBLIC HEALTH
(2022)
Meeting Abstract
Public, Environmental & Occupational Health
G. Guarducci, I. Sanguineti, C. Cuccaro, R. Randisi, G. Messina, N. Nante
EUROPEAN JOURNAL OF PUBLIC HEALTH
(2022)
Meeting Abstract
Public, Environmental & Occupational Health
G. Guarducci, A. Urbani, S. Carbone, F. Moirano, G. Messina, N. Nante
EUROPEAN JOURNAL OF PUBLIC HEALTH
(2022)
