Journal
JOURNAL OF FORENSIC SCIENCES
Volume 64, Issue 2, Pages 593-601Publisher
WILEY
DOI: 10.1111/1556-4029.13869
Keywords
forensic science; forensic entomology; DNA barcoding; high throughput sequencing; next generation sequencing; Cytochrome C Oxidase 1; DNA reference library; bulk sample analysis
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Funding
- German Federal Ministry of Education and Research [FKZ 01LI1101, 01LI1501]
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Throughout the years, DNA barcoding has gained in importance in forensic entomology as it leads to fast and reliable species determination. High-quality results, however, can only be achieved with a comprehensive DNA barcode reference database at hand. In collaboration with the Bavarian State Criminal Police Office, we have initiated at the Bavarian State Collection of Zoology the establishment of a reference library containing arthropods of potential forensic relevance to be used for DNA barcoding applications. CO1-5P' DNA barcode sequences of hundreds of arthropods were obtained via DNA extraction, PCR and Sanger Sequencing, leading to the establishment of a database containing 502 high-quality sequences which provide coverage for 88 arthropod species. Furthermore, we demonstrate an application example of this library using it as a backbone to a high throughput sequencing analysis of arthropod bulk samples collected from human corpses, which enabled the identification of 31 different arthropod Barcode Index Numbers.
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