Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 821, Issue -, Pages 87-91Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2017.11.028
Keywords
Electrochemical; DNA-based biosensor; External protective membrane; Acridines; DNA intercalators; Urine
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Funding
- Scientific Grant Agency VEGA of the Slovak Republic [1/0489/16]
- Competence Center for SMART Technologies for Electronics and Informatics Systems and Services - Research & Development Operational Programme from the ERDF [ITMS 26240220072]
- STU
- Grant Agency of the Czech Republic [P206/12/G151]
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The aim of this work is preparation and use of an electrochemical DNA-based biosensor with a textured surface for the investigation of dsDNA host-guest interactions with specific chemicals in a biological sample of complex matrix such as urine. Results obtained at the search and optimization of polymer protective films over the DNA recognition layer at a glassy carbon electrode show that a polyvinyl alcohol membrane is the effective barrier able to stabilize the biosensor response in urine. The detection of acridines using the square-wave voltammetric (SWV) scan in the cathodic direction has allowed overcoming a problem of the electrochemical interference of DNA bases and acridines at the direct anodic scan. Height of the well-separated SWV peak at around 0.00 V to + 0.10 V (which corresponds to the reduction of the oxidation products of both of acridines) depends on the concentration of acridines. The peak potentials of acridines were shifted by about 0.10 V to the negative potential values in the urine medium compared to phosphate buffer. Intercalation of acridine yellow and proflavine into dsDNA was confirmed both in phosphate buffer and in urine.
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