Journal
JOURNAL OF DENTAL RESEARCH
Volume 97, Issue 11, Pages 1236-1243Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/0022034518770605
Keywords
tsRNAs; cross-domain interactions; oral microbiome; antimicrobials; microbial-host interaction; sRNAs
Categories
Funding
- National Institute of Dental and Craniofacial Research [1R01DE023810, 1R01DE020102, 1R01DE026186]
- National Institutes of Health [UH3 TR000923, R01HG006264, F32DE025548-1]
- NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES [UH3TR000923] Funding Source: NIH RePORTER
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG006264] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [R01DE020102, R01DE023810, F32DE025548, R01DE026186] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Coevolution of the human host and its associated microbiota has led to sophisticated interactions to maintain a delicate homeostasis. Emerging evidence suggests that in addition to small molecules, peptides, and proteins, small regulatory noncoding RNAs (sRNAs) might play an important role in cross-domain interactions. In this study, we revealed the presence of diverse host transfer RNA-derived small RNAs (tsRNAs) among human salivary sRNAs. We selected 2 tsRNAs (tsRNA-000794 and tsRNA-020498) for further study based on their high sequence similarity to specific tRNAs from a group of Gram-negative oral bacteria, including Fusobacterium nucleatum, a key oral commensal and opportunistic pathogen. We showed that the presence of F. nucleatum triggers exosome-mediated release of tsRNA-000794 and tsRNA-020498 by human normal oral keratinocyte cells. Furthermore, both tsRNA candidates exerted a growth inhibition effect on F. nucleatum, likely through interference with bacterial protein biosynthesis, but did not affect the growth of Streptococcus mitis, a health-associated oral Gram-positive bacterium whose genome does not carry sequences bearing high similarity to either tsRNA. Our data provide the first line of evidence for the modulatory role of host-derived tsRNAs in the microbial-host interaction.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available