Journal
JOURNAL OF DAIRY SCIENCE
Volume 101, Issue 3, Pages 1915-1920Publisher
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2017-13961
Keywords
Lactobacillus casei Zhang; proteomics; gentamycin; membrane-associated protein; asp23 gene
Funding
- Natural Science Foundation of Inner Mongolia [2017ZD07]
- Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region [NJYT-17-B05]
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Lactobacillus casei Zhang is a probiotic strain originally isolated from koumiss. Previously, we showed that an alkaline shock protein (encoded by asp23) was involved in the adaptation of L. casei Zhang to gentamycin. In the present study, we compared the proteomes of the asp23 mutant and its parent strain grown in the presence of gentamycin. The results showed that 22 and 21 proteins were significantly up-and downregulated, respectively (> 1.5-fold difference). By parallel reaction monitoring analysis, we further validated that specific membrane-associated proteins were important in regulating the antibiotics-induced cell wall stress. The findings provide insight into the physiological role of the asp23 gene in the growth response of L. casei when exposed to antibiotic stress.
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