Article
Food Science & Technology
Guoyang Xie, Donggen Zhou, Guojing Zhao, Xiaoyan Feng, Zoraida P. Aguilar, Hengyi Xu
Summary: The study proposed a method for rapid and real-time detection of viable S. aureus in milk, combining PMAxx dye and RAA technology. It can accurately detect S. aureus in a short time, which is of great significance for effective monitoring of food safety.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Wei Guo, Qian Yang, Yunzhe Zhang, Xin Lu, Chenchen Wu, Jianxin Tan, Wei Zhang
Summary: The aim of this study was to establish a rapid and visual nucleic acid isothermal amplification method for the detection of viable Staphylococcus aureus (S. aureus) in pork and pork products. The PMA-SRCA assay showed a higher sensitivity and reduced the false-positive detection rate of S. aureus in pork and pork products.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Alka Rani, Charles Chinyere Dike, Nitin Mantri, Andrew Ball
Summary: This study developed a rapid and reliable method for the detection of viable Escherichia coli O157:H7 using recombinase polymerase amplification (RPA) combined with propidium monoazide (PMAxx). The assay was able to differentiate between viable/dead bacteria and detect VBNC cells. It showed good performance in terms of detection limit, applicability, and pH stability.
Article
Food Science & Technology
Jingbin Li, Donggen Zhou, Guoyang Xie, Mei Deng, Xiaoyan Feng, Hengyi Xu
Summary: In this study, a PMAxx-RAA assay was developed to rapidly and sensitively detect Salmonella in milk. The assay is specific and can eliminate false positive results.
FOOD ANALYTICAL METHODS
(2022)
Article
Food Science & Technology
Liyun Lin, Guangcai Zha, Huagui Wei, Yuzhong Zheng, Peikui Yang, Yaqun Liu, Mouquan Liu, Zhonghe Wang, Xianghui Zou, Hui Zhu, Qiulan Luo, JinQuan Li, Min Lin
Summary: The study developed a detection method for Staphylococcus aureus in food using a combination of recombinase polymerase amplification (RPA) and CRISPR-Cas12a technology. The method demonstrated high sensitivity in detecting low quantities of S. aureus and proved feasible for use in both laboratory and field samples. This accurate and portable detection method has great potential in food safety applications.
Article
Microbiology
Chuangxin Lin, Yongmei Zeng, Zhihong Zhu, Jiayu Liao, Tiandan Yang, Yaqun Liu, Huagui Wei, Jiamin Li, Jibin Ma, Xiaoqing Wu, Guangyu Lin, Liyun Lin, Liying Chen, Huiying Huang, Weizhong Chen, Junli Wang, Feiqiu Wen, Min Lin
Summary: Antimicrobial resistance (AMR) is a global threat to public health, and there is a need for rapid and accurate detection of AMR in Staphylococcus aureus. In this study, two versions of recombinase polymerase amplification (RPA) were developed to detect AMR genes and identify S. aureus isolates. Clinical samples validated the high sensitivity, specificity, and accuracy of the RPA tool, which showed 100% consistency with PCR results. Overall, this rapid and accurate AMR diagnostic platform can improve antibiotic therapy in clinical microbiology laboratories.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Xiaoying Cao, Yanbin Chang, Chunqing Tao, Sen Chen, Qiuxia Lin, Chao Ling, Shifeng Huang, Hengshu Zhang
Summary: In order to prevent and control MRSA infection, a fast and accurate nucleic acid detection platform was developed for identifying S. aureus and MRSA. The study constructed a CRISPR-Cas12a detection system paired with PCR, LAMP, and RPA to increase sensitivity. After comparing the different methods, the nuc-LAMP-Cas12a and mecA-LAMP-Cas12a platforms were determined to be the optimal detection methods with high specificity and sensitivity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Xiaoyan Feng, Donggen Zhou, Dan Mu, Hengyi Xu
Summary: Escherichia coli O157:H7 is a harmful bacterium that produces toxins causing thrombotic thrombocytopenic purpura and haemolytic uraemic syndrome. Real-time recombinase-aided amplification (rRAA) can detect pathogens within 20 min but cannot differentiate between dead and viable bacteria, leading to false-positive results. By integrating thiazole orange monoazide (TOMA) with rRAA, a sensitive assay was developed, allowing for rapid identification of viable E. coli O157:H7 in milk with a detection limit of less than 10 cfu mL-1, and resistance to interference from non-target bacteria and high concentrations of dead bacteria.
INTERNATIONAL DAIRY JOURNAL
(2023)
Article
Microbiology
Soumana Daddy Gaoh, Ohgew Kweon, Youngbeom Ahn
Summary: Both sterile and non-sterile pharmaceutical products, including antiseptics, have been recalled due to contamination by Burkholderia cepacia complex (BCC). This study evaluates the use of an exo probe-based recombinase polymerase amplification (RPA) with propidium monoazide (PMAxx) for selective detection of live/dead BCC cells. The results show that the PMAxx-RPA exo assay is a valid tool for the simple, rapid, and presumptive detection of live BCC cells in antiseptics, ensuring the quality and safety of pharmaceutical products.
Article
Chemistry, Analytical
Marko Pavlovic, Heman Kumar Ramiya Ramesh Babu, Saveh Djalali, Milan Vranes, Vasa Radoni, Lukas Zeininger
Summary: A novel method for monitoring mineral content of drinking water using responsive complex emulsions was reported, showing changes in droplet morphology to reflect variations in surfactant effectiveness due to metal cation addition. The study demonstrated that salt concentration and valency can influence the extent of droplet response, with divalent cations resulting in a more pronounced response. This device concept may provide a simple and cost-effective alternative for rapid monitoring of water hardness levels.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Composites
Wei Hu, Xinlin Zha, Nian Liu, Siqi Ma, Xin Liu, Ming Xia, Yonggang Yang, Yuanli Chen, Ke Liu, Dong Wang
Summary: In this study, a new method was developed to fabricate highly permeable polyamide nanocomposite membranes by adding silica nanorods and sodium dodecyl sulfate solution on the surface of EVOH nanofibrous scaffold through interfacial polymerization. The resulting membranes exhibited a foam-like polyamide layer with a hierarchical internal voids structure, showing excellent performance in terms of permeance.
COMPOSITES COMMUNICATIONS
(2022)
Article
Materials Science, Ceramics
Shuai Xie, Mingliang Yuan, Tanxin Wang, Jingjun Liu, Junqing Yan, Zhen Li, Jing Peng
Summary: In this study, homogeneous monodisperse manganese carbonate (MnCO3) microspheres were synthesized using a co-precipitation method and their dispersion characteristics were investigated using different surfactants. The results showed that the addition of anionic surfactant SDS enabled the synthesis of MnCO3 microspheres with superior tap density. Furthermore, LiMn2O4 cathode material synthesized using spherical MnCO3 as a template exhibited excellent electrochemical performance.
CERAMICS INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Yao Liu, Caijiao Wei, Hui Wan, Sarengaowa Sarengaowa, Xiaoping Liang, Tao Jiang, Yuhe Dong, Xihong Zhao, Tian Zhong
Summary: A rapid and practical technique (PMA-mPCR) was developed for the simultaneous detection of viable E. coli O157:H7, S. aureus, and Salmonella in pure culture and in a food matrix. The use of propidium monoazide (PMA) at an optimum concentration of 5.0 μg/mL successfully eliminated false positive results. The PMA-mPCR assay showed a detection limit of 10(4) CFU/mL for both pure culture and food matrix samples.
Article
Chemistry, Analytical
Xingzhi Wu, Dan Jin, Wei Ni, Feng Wang, Yuling Zhang, Guo-Jun Zhang
Summary: Rapid and accurate identification of Methicillin-resistant Staphylococcus aureus (MRSA) is crucial to prevent the spread of infections and guide effective treatment. In this study, a rapid, specific, and ultrasensitive colorimetric assay based on recombinase polymerase amplification (RPA) and magnetic nanoparticles (MNPs) assisted CRISPR/Cas12a was developed for the detection of the mecA gene in MRSA bacteria. This method allows for the rapid and accurate identification of MRSA within 2 hours through the observable color change.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Microbiology
Runan Cheng, Lei Li, Sihui Zhen, Honglei Liu, Zhouhui Wu, Yu Wang, Zhen Wang
Summary: In this study, a novel method based on immunomagnetic separation using immunomagnetic beads (IMBs) and recombinase polymerase amplification (RPA) was developed for the rapid detection of Staphylococcus aureus (S. aureus) in milk and pork. The IMBs-RPA method showed potential for food safety supervision and rapid early diagnosis of diseases due to its short detection time, high sensitivity, and specificity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yankun Zhao, He Chen, Huimin Li, Jianxing Cai, Lu Meng, Lei Dong, Nan Zheng, Jiaqi Wang, Cheng Wang
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Agriculture, Dairy & Animal Science
Lei Dong, Huimin Liu, Lu Meng, Mengru Xing, Tu Lan, Mei Gu, Nan Zheng, Cheng Wang, He Chen, Jiaqi Wang
JOURNAL OF DAIRY SCIENCE
(2019)
Article
Multidisciplinary Sciences
Cheng Wang, Weizhong He, Lu Kang, Song Yu, Aibo Wu, Wenliang Wu
Article
Food Science & Technology
Dongqiang Hu, Song Yu, Dianzhen Yu, Na Liu, Yan Tang, Yingying Fan, Cheng Wang, Aibo Wu
Article
Chemistry, Applied
Cheng Wang, Wei-Zhong He, Duo-Yong Zhao, Zhi Liu, Ying-ying Fan, Wei-Na Tian, Wen-Liang Wu, Karyne M. Rogers
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2020)
Article
Food Science & Technology
Fengjuan Liu, Wenshu Huang, Zuoshan Feng, Yongxia Tao, Yingying Fan, Weizhong He, XiaoLi Li, Xiaotong Fang, Cheng Wang, Yujia Bai
Summary: This study conducted protein profiling of fresh and shade-dried Thompson seedless grapes using data independent acquisition technology, identifying key proteins involved in browning process and revealing their roles in the mechanism of color change.
APPLIED BIOLOGICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Lan Wang, Zheng Yan, Haiyan Zhou, Yingying Fan, Cheng Wang, Jingbo Zhang, Yucai Liao, Aibo Wu
Summary: A developed LC-MS/MS method was effective in chemotype determination of Fusarium strains, allowing successful chromatographic separation of 3-ADON and 15-ADON with good stability and accuracy.
Article
Food Science & Technology
Lu Kang, Hejiang Liu, Duoyong Zhao, Canping Pan, Cheng Wang
Summary: The study investigates pesticide levels in celery and how they degrade after being sprayed with selenium nanoparticles. Results show that the degradation rate of pesticides in celery is accelerated, with reduced half-lives, and dietary exposure risk remains within acceptable limits, except for abamectin.
Article
Chemistry, Applied
Yingying Fan, Fengjuan Liu, Weizhong He, Qiaomei Qin, Dongqiang Hu, Aibo Wu, Weibo Jiang, Cheng Wang
Summary: An improved method was developed for the detection of mycotoxins in fruit samples from Xinjiang, providing accurate data and revealing significant contamination with mycotoxins, with the highest levels of three Alternaria toxins found in jujube, wolfberries, and raisins.
Article
Biochemistry & Molecular Biology
Fan Sun, Xueqiang Cao, Dianzhen Yu, Dongqiang Hu, Zheng Yan, Yingying Fan, Cheng Wang, Aibo Wu
Summary: By studying two genes, AaTAS1 and AaMFS1, in Alternaria alternata, this research reveals their significant roles in the biosynthesis and transmembrane transport of tenuazonic acid (TeA) in the pathogenic fungus Pyricularia oryzae. It also confirms their association with the pathogenicity of the fungus.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Microbiology
Lingqiao Chen, Haoyu Wang, Junhua Yang, Xianli Yang, Mengyuan Zhang, Zhihui Zhao, Yingying Fan, Cheng Wang, Jianhua Wang
Summary: This study identified 23 FgCFEM proteins from the F. graminearum genome and analyzed their features and functions. It was found that each FgCFEM protein contains one CFEM domain with highly conserved amino acids but some variations. Transcriptome analysis revealed the expression of many CFEM-containing genes during wheat infection.
Article
Chemistry, Applied
Li Bin, Cheng Wang, Zhi Liu, Weizhong He, Duoyong Zhao, Ying-ying Fang, Ying Li, Zihong Zhang, Piao Chen, Wei Liu, Karyne M. Rogers
Summary: The geographical origin of Xinjiang muskmelon was investigated using stable isotopes and multi-element analyses. A linear discriminant analysis model was developed to classify the muskmelon from different geographical production regions in Xinjiang. This method is significant for combating origin mislabeling of Hami PGI muskmelons and ensuring the integrity of food.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Microbiology
Huimin Liu, Lei Dong, Yankun Zhao, Lu Meng, Jiaqi Wang, Cheng Wang, Nan Zheng
Summary: This study investigated the prevalence, antimicrobial susceptibility, and virulence genes of Staphylococcus aureus in different raw milks in China. The results showed a high prevalence of Staphylococcus aureus in goat milk, with most strains being multidrug resistant and carrying multiple virulence genes, posing potential risk to public health.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Xupeng Shao, Weizhong He, Yingying Fan, Qi Shen, Jiancai Mao, Meihua Li, Guozhi Hu, Fengjuan Liu, Cheng Wang
Summary: This study analyzed the differences in aroma components of Nasmi melons from two different production regions and identified the key genes causing these differences. The study provides a theoretical basis for further research on the formation mechanism of melon aroma and breeding.
FOOD SCIENCE & NUTRITION
(2022)
Article
Biophysics
Xiao Chen, Wenqin Wu, Qi Zhang, Cheng Wang, Yingying Fan, Huimin Wu, Zhaowei Zhang
Summary: In this study, a highly active Bi2O3/CuBi2O4 photocathode biosensor was successfully synthesized, and its potential application in small molecular immunosensing was verified.
BIOSENSORS & BIOELECTRONICS
(2022)
