Review
Biotechnology & Applied Microbiology
Zeqing Zhao, Jin Liu, Michael D. Weir, Abraham Schneider, Tao Ma, Thomas W. Oates, Hockin H. K. Xu, Ke Zhang, Yuxing Bai
Summary: This review article presents the recent advances in PDLSC-based bone regeneration and periodontal tissue regeneration. By combining PDLSCs with various materials and scaffolds, as well as applying different osteoinductive agents and strategies, PDLSCs demonstrate the potential for bone and periodontal tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Xuanwen Xu, Yi Zhou, Kai Zheng, Xinyu Li, Lu Li, Yan Xu
Summary: Periodontitis is a chronic inflammatory disease that affects people worldwide and has an uncertain prognosis with surgical treatment. In this study, a three-dimensional scaffold made of electrospun polycaprolactone/gelatin fibrous membranes was developed to overcome the challenges of periodontal treatment. The scaffold showed good properties and promoted adhesion and proliferation of human periodontal ligament stem cells. In vivo experiments confirmed that the scaffold could guide cellular orientation and potentially facilitate periodontal regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Cell Biology
Gustavo Henrique Dona Rodrigues Almeida, Leandro Norberto da Silva-Junior, Mariana Sversut Gibin, Henrique dos Santos, Bianca de Oliveira Horvath-Pereira, Leticia Beatriz Mazo Pinho, Mauro Luciano Baesso, Francielle Sato, Luzmarina Hernandes, Charles R. Long, Luciana Relly, Maria Angelica Miglino, Ana Claudia Oliveira Carreira
Summary: This study aimed to establish and standardize a protocol for porcine ovaries' decellularization based on detergent perfusion and ultrasonication to obtain functional whole-ovary scaffolds. Results showed that the proposed method was able to remove cellular components efficiently, and the scaffolds had preserved ECM composition, were cytocompatible, and allowed cell attachment.
Article
Biochemistry & Molecular Biology
Hsu-Hsiang Lin, Pen-Hsiu Grace Chao, Wei-Chiu Tai, Po-Chun Chang
Summary: This study successfully developed a biomimetic microfibrous system to assist PDL regeneration and withstand functional load. Collagen-based microfibers were fabricated and PDL cells seeded on waveform microfibers showed enhanced growth and gene expression levels, indicating potential for promoting healing and regeneration under shear stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Nanoscience & Nanotechnology
Qi You, Minxun Lu, Zhuangzhuang Li, Yong Zhou, Chongqi Tu
Summary: Congenital or acquired bone defects present a challenge to the global healthcare system, and novel cell sheet technology shows promise for promoting bone regeneration. This review introduces and explores the application of cell sheet technology in bone regeneration, highlights the current state of research, and offers perspectives on translating this knowledge to clinical practice.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Engineering, Environmental
Yaoshan Liu, Tong Zhang, Min Li, Zhaoguang Ouyang, Feng Gao, Chunyi Liu, Chen Li, Dayong Liu, Zhimin Zhou
Summary: This study focused on the potential of polymer porous microspheres (PMs) as stem cells delivery carriers for periodontal regeneration. The synthesized PLGA PMs, along with SF and/or HA modifications, were used to seed hPDLSCs with specific techniques. In vitro evaluations showed promising results for cell viability, migration, proliferation, and osteogenic differentiation. In vivo experiments demonstrated the superior tissue repairing efficacy of the HA-SF-PLGA group due to SF-dependent adhesion and HA-dependent osteogenesis.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Biomedical
Mahboubeh Sadat Mousavi, Ghassem Amoabediny, Seyed Hossein Mahfouzi, Seyed Hamid Safiabadi Tali
Summary: The study introduced a novel perfusion-based bioreactor (PBB) method for efficient decellularization of bovine articular cartilages, resulting in better preservation of the tissue structure and essential components while reducing harmful physical and chemical steps and process duration. The PBB method showed a 90% reduction in DNA content, significantly higher than the control method, and maintained around 50% of sulfated glycosaminoglycan (sGAG) content and 92% of compression properties, demonstrating efficient preservation of scaffold structure and components. Additionally, the PBB specimens supported cell adherence and proliferation effectively, showing promise for producing close-to-natural scaffolds for cartilage tissue engineering.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Review
Engineering, Biomedical
Yexin Li, Ting Deng, Dilihumaer Aili, Yang Chen, Weihong Zhu, Qian Liu
Summary: This review summarizes the application of cell sheet technology in the treatment of tendon and ligament injuries, focusing on the effects of stem cell-derived sheets in mitigating inflammation and promoting healing. It also discusses the prerequisites for future clinical translation.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Engineering, Biomedical
Minji Choi, Seongsu Park, Sohanur Rahaman, Garima Tripathi, Byong-Taek Lee
Summary: The study investigated the co-culture of rGEC and rBMSC in kidney scaffolds cultured by a bioreactor system, leading to increased cell proliferation and microvascularization, and reduced inflammation and cell death caused by flow injury.
MATERIALS TODAY BIO
(2022)
Article
Cell Biology
Ingrid Zahn, Daniel David Stoebener, Marie Weinhart, Clemens Goegele, Annette Breier, Judith Hahn, Michaela Schroepfer, Michael Meyer, Gundula Schulze-Tanzil
Summary: The use of thermoresponsive polymer PGE coating improves the efficiency of ACL tissue engineering by promoting cell sheet formation and maintaining relevant cell phenotypes.
Article
Biochemistry & Molecular Biology
Olimpia Ortiz-Arrabal, Ramon Carmona, Oscar-Dario Garcia-Garcia, Jesus Chato-Astrain, David Sanchez-Porras, Alberto Domezain, Roke-Inaki Oruezabal, Victor Carriel, Antonio Campos, Miguel Alaminos
Summary: Research on a novel decellularized sturgeon cartilage scaffold showed promising results in terms of biocompatibility and sustainability for chondrocytes, as well as triggering a pro-regenerative reaction. This suggests potential for further development of these cartilage substitutes for clinical translation.
Review
Dentistry, Oral Surgery & Medicine
Fabiana Divina Magalhaes, Giovanna Sarra, Giovanna Lopes Carvalho, Ana Clara Fagundes Pedroni, Marcia Martins Marques, Leandro Chambrone, Thais Gimenez, Maria Stella Moreira
Summary: The review of 33 studies indicates that cell sheets formed by mesenchymal stem cells derived from dental tissues have great potential for periodontal tissue regeneration in animal models, but the regeneration process is not always complete.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ifeolu Akinnola, Daniel R. Rossi, Carolyn Meyer, Ashley Lindsey, Douglas R. Haase, Samuel Fogas, Michael J. Ehrhardt, Rachel E. Blue, Andrew P. Price, Max Johnson, Diego F. Alvarez, Doris A. Taylor, Angela Panoskaltsis-Mortari
Summary: This study evaluated the recellularization of blood vessel conduits of acellular lung scaffolds with different types of endothelial cells, showing that RMEPCs have the capability to repopulate all vascular segments, while PAECs and MVECs exhibit selective tropism for larger vessels or microvasculature, respectively. The RMEPC phenotype described in the study could be valuable for identifying suitable endothelial progenitors for efficient vascular organ and tissue engineering, regeneration, and repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ju An Park, Yejin Youm, Hwa-Rim Lee, Yongwoo Lee, Sarah L. Barron, Taejeong Kwak, Gyu Tae Park, Young-Cheol Song, Roisin M. Owens, Jae Ho Kim, Sungjune Jung
Summary: A cell sheet transfer method from a culture surface to a target tissue is introduced in this study. UV-treated parylene, a commercially available flexible material, is used as the culture surface to provide adequate cell adhesiveness for both cell attachment during culture and efficient transfer. Various cell types are successfully transferred using this versatile cell-transfer process. The mechanism of interfacial cell migration between surfaces with different adhesion preferences is observed, and the re-establishment and reversal of cell polarity after transfer are identified. The unique capabilities of 3D multilayer stacking, freeform design, and curved surface application are demonstrated. The therapeutic potential of the cell-sheet delivery system is demonstrated through experiments on cutaneous wound healing and skin-tissue regeneration in mice models.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Yuriy Snyder, Soumen Jana
Summary: Valvular heart diseases are commonly treated using mechanical or bioprosthetic heart valves, but these valves have limitations. Heart valve tissue engineering offers a potential solution by using scaffolds that can grow with patients and function without the need for lifelong anticoagulation medication.
Review
Engineering, Biomedical
Fanny Blaudez, Saso Ivanovski, Benjamin Fournier, Cedryck Vaquette
Summary: Recent advancements in regenerative medicine and biomaterial science have emphasized the importance of regulating immune cell phenotypes at the interface of biomaterials. One promising approach is the use of reparative biological cues called resolvins, which have shown to play a key role in the resolution of inflammation and tissue regeneration. This review provides a comprehensive overview of the mechanisms and pathways involving resolvins, their current utilization in pre-clinical and clinical settings, and their potential when combined with biomaterial constructs in tissue engineering applications.
ACTA BIOMATERIALIA
(2022)
Article
Surgery
George Castrisos, Isabel Gonzalez Matheus, David Sparks, Martin Lowe, Nicola Ward, Marjoree Sehu, Marie-Luise Wille, Yun Phua, Flavia Medeiros Savi, Dietmar Hutmacher, Michael Wagels
Summary: This study presents a novel bone replacement technique based on regenerative matching axial vascularisation (RMAV) and its clinical applications. The technique utilizes 3D-printed medical-grade polycaprolactone and tricalcium phosphate (mPCL-TCP) scaffolds wrapped in vascularised free corticoperiosteal flaps for bone defect reconstruction, achieving successful outcomes.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2022)
Article
Cell & Tissue Engineering
David S. Sparks, Flavia Medeiros Savi, Siamak Saifzadeh, Marie-Luise Wille, Michael Wagels, Dietmar W. Hutmacher
Summary: This study presents a novel approach for bone healing in critical size bone defects using a corticoperiosteal flap. The method has been proven effective in a large animal model and demonstrates potential for clinical application.
TISSUE ENGINEERING PART C-METHODS
(2022)
Article
Engineering, Biomedical
Reuben Staples, Saso Ivanovski, Cedryck Vaquette
Summary: This study demonstrates the potential of a highly porous scaffold with 100 μm width channels manufactured by melt electrowriting to induce perpendicular alignment and attachment of the periodontal ligament, leading to enhanced periodontal tissue regeneration.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Markus Laubach, Philipp Kobbe, Dietmar W. Hutmacher
Summary: Lumbar fusion is a common treatment for spinal diseases, but current cage materials have limitations. Recently, biodegradable cages and scaffold-guided bone regeneration have shown potential for better outcomes. Surface modification of implants can also enhance tissue integration and reduce infections. Further research is needed to improve these techniques.
Review
Multidisciplinary Sciences
B. L. Dargaville, D. W. Hutmacher
Summary: Water plays a crucial role in controlling physical, chemical, and biological processes, particularly in materials such as hydrogels. However, there is still much to be understood about how water interacts with these materials and its impact on biological processes. This perspective outlines the importance of water in biomaterials science and provides directions for future research.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Nimal Raveendran, Saso Ivanovski, Cedryck Vaquette
Summary: 3D bioprinting allows the incorporation of cells in a porous construct and enhances bone-related gene expression. The combination of pre-induction and post-differentiation regimens shows superior osteogenic potential of bioprinted constructs in vitro and in vivo. This study reveals the positive impact of bioprinting on osteogenic differentiation and the potential for bone regeneration.
ACTA BIOMATERIALIA
(2023)
Article
Health Care Sciences & Services
Isabel Gonzalez Matheus, Dietmar W. Hutmacher, Sarah Olson, Michael Redmond, Allison Sutherland, Michael Wagels
Summary: This study aims to investigate the reconstructive capacity of a hybridized technique for large skull defects. The technique combines autologous and synthetic materials to promote bone regeneration through a biodegradable 3D-printed scaffold wrapped in vascularized autotransplanted periosteum. The results of the study are expected to be available before the end of 2023.
JMIR RESEARCH PROTOCOLS
(2022)
Article
Engineering, Biomedical
Agathe Bessot, Jennifer Gunter, David Waugh, Judith A. Clements, Dietmar W. Hutmacher, Jacqui McGovern, Nathalie Bock
Summary: Increasing evidence highlights the important role of bone marrow adipocytes in prostate cancer bone metastases. However, lack of relevant models has hindered the understanding of human BM-adipocyte effects in this microenvironment. This study combines tunable GelMA-based hydrogels with biomimetic culture of human cells to engineer human bone tumor microenvironments containing BM-adipocytes. In vitro culture of human osteoprogenitors, adipocytes, and PCa cells in GelMA hydrogels leads to the formation of mineralized, adipose, and PCa tumor 3D microtissues, respectively. The stiffness of hydrogels regulates osteoblast mineralization and tumor spheroid formation, while coculture with tumor cells induces morphological changes and delipidation in BM-adipocytes. The in vivo model successfully forms a humanized fatty bone microenvironment and demonstrates that adipocytes support early bone tumor growth. This advanced platform combines natural aspects of the microenvironment with tunable properties for bone tumor research.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Tara Shabab, Onur Bas, Bronwin L. Dargaville, Akhilandeshwari Ravichandran, Phong A. Tran, Dietmar W. Hutmacher
Summary: This study combines melt-extrusion based 3D printing and porogen leaching to develop tunable multiphasic scaffolds for dental tissue regeneration. The scaffolds are highly adjustable in terms of mechanical properties, degradation kinetics, and surface morphology. The research also demonstrates improved cell attachment, proliferation, and extracellular matrix production on these multiscale scaffolds compared to single-scale counterparts, indicating their potential for enhanced tissue regeneration. Furthermore, the study explores the use of these scaffolds as drug delivery devices and shows that sustained drug release can be achieved. These findings strongly support further development of these scaffolds for dental tissue regeneration applications.
Article
Biochemistry & Molecular Biology
Fanny Blaudez, Saso Ivanovski, Tulio Fernandez, Cedryck Vaquette
Summary: Recent research examined the impact of in vitro culture on the bioactivity of ECM-decorated scaffolds for bone regeneration. It was found that longer culture periods hindered cell infiltration and overall bone regeneration, possibly due to pore obstruction caused by prolonged culture. This study highlights the importance of culture length in the development of effective ECM-decorated scaffolds.
Article
Engineering, Manufacturing
Buddhi Herath, Markus Laubach, Sinduja Suresh, Beat Schmutz, J. Paige Little, Prasad K. D. V. Yarlagadda, Dietmar W. Hutmacher, Marie-Luise Wille
Summary: A modular design workflow was developed to facilitate the efficient design and manufacture of patient-specific scaffolds for scaffold-guided bone regeneration (SGBR). The workflow allows for semi-automatic filling of defect cavities, patient specificity, ideal surgical scaffold insertion, and generation of scaffolds based on various pore architectures. The real-time feedback from surgeons shortens the time required to finalize a patient-specific scaffold design.
VIRTUAL AND PHYSICAL PROTOTYPING
(2023)
Review
Engineering, Biomedical
Markus Laubach, Frank Hildebrand, Sinduja Suresh, Michael Wagels, Philipp Kobbe, Fabian Gilbert, Ulrich Kneser, Boris M. Holzapfel, Dietmar W. Hutmacher
Summary: The treatment of bone defects is a challenging clinical problem with high reintervention rates and significant healthcare costs. Three-dimensional printing has revolutionized the treatment of bone defects by enabling personalized therapies with highly porous 3D-printed implants. However, there are still challenges to be addressed, including the development of guidelines, clinical trial design, and financing support.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Mairim Russo Serafini, Alexandra Mowat, Susanah Mustafa, Siamak Saifzadeh, Tara Shabab, Onur Bas, Nicholas O'Rourke, Dietmar W. Hutmacher, Flavia Medeiros Savi
Summary: This study evaluated the use of mPCL scaffolds loaded with PRP for hernia repair in sheep models, and found that the mPCL scaffolds enhanced soft tissue regeneration. After 6 months, the mPCL scaffolds showed similar mechanical properties, collagen, and vascular ingrowth compared to PP meshes. This pilot study supports the use of mPCL as a safe and effective biodegradable scaffold for hernia and vaginal prolapse repair.
BIOENGINEERING-BASEL
(2023)
Article
Orthopedics
Markus Laubach, Sinduja Suresh, Buddhi Herath, Marie-Luise Wille, Heide Delbrueck, Hatem Alabdulrahman, Dietmar W. Hutmacher, Frank Hildebrand
Summary: This study reports the successful application of patient-specific SGBR in the treatment of large complex long bone defects, highlighting the importance of scaffold design in promoting bone regeneration.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
