Article
Biotechnology & Applied Microbiology
Yunlong Tao, Xueyan Li, Qiping Dong, Linghai Kong, Andrew J. Petersen, Yuanwei Yan, Ke Xu, Seth Zima, Yanru Li, Danielle K. Schmidt, Melvin Ayala, Sakthikumar Mathivanan, Andre M. M. Sousa, Qiang Chang, Su-Chun Zhang
Summary: Researchers have successfully generated central norepinephrine (NE) neurons from stem cells, providing a tool for studying their roles in psychiatric and neurodegenerative diseases.
NATURE BIOTECHNOLOGY
(2023)
Article
Cell Biology
Stephanie A. Luff, J. Philip Creamer, Sara Valsoni, Carissa Dege, Rebecca Scarfo, Analisa Dacunto, Sara Cascione, Lauren N. Randolph, Eleonora Cavalca, Ivan Merelli, Samantha A. Morris, Andrea Ditadi, Christopher M. Sturgeon
Summary: Luff et al. discovered a distinct human mesoderm cell population that generates mature haemogenic endothelium in a retinoic acid-dependent manner. This finding highlights the importance of retinoic acid-responsive mesoderm in haematopoietic ontogeny. The revised model of human haematopoietic development provides essential resolution to the regulation and origins of the multiple waves of haematopoiesis.
NATURE CELL BIOLOGY
(2022)
Review
Cell & Tissue Engineering
Chunmeng Yao, Ruqiang Yao, Haining Luo, Ling Shuai
Summary: This review describes in vitro germline specification studies, focusing on the reconstitution of PGCs, meiosis, signaling pathways, and factors involved in these processes.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Marina Sanaki-Matsumiya, Mitsuhiro Matsuda, Nicola Gritti, Fumio Nakaki, James Sharpe, Vikas Trivedi, Miki Ebisuya
Summary: This study reports the generation of human somitoids, organoids that periodically form epithelial somite-like structures. Somitoids display oscillations of the segmentation clock and have proper polarity.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemical Research Methods
Min Shi, Ping Fu, Joseph V. Bonventre, Kyle W. McCracken
Summary: Developing in vitro models of human kidney tissue using human pluripotent stem cells (hPSCs) is challenging but essential for regenerative nephrology research. This article describes a step-by-step protocol for differentiating hPSCs into three-dimensional ureteric bud (UB) organoids, which exhibit complex morphological development and can differentiate into functional collecting duct tissues. This approach provides a robust and reproducible method for generating UB tissues that can be used to study kidney development, model disease processes, and aid in the engineering of functional kidney tissue.
Article
Multidisciplinary Sciences
Mami Oikawa, Hisato Kobayashi, Makoto Sanbo, Naoaki Mizuno, Kenyu Iwatsuki, Tomoya Takashima, Keiko Yamauchi, Fumika Yoshida, Takuya Yamamoto, Takashi Shinohara, Hiromitsu Nakauchi, Kazuki Kurimoto, Masumi Hirabayashi, Toshihiro Kobayashi
Summary: The in vitro generation of germ cells from pluripotent stem cells has significant implications for reproductive medicine and animal breeding. In this study, researchers successfully induced functional primordial germ cell-like cells from rat pluripotent stem cells and generated functional spermatids through transplantation. This research provides insights into the mechanisms of in vitro gametogenesis and has implications for broader applications.
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Cell & Tissue Engineering
Hongli Jiao, Brian E. Walczak, Ming-Song Lee, Madeleine E. Lemieux, Wan-Ju Li
Summary: Cellular reprogramming can reverse aging of cells by altering the activities of regulatory networks associated with inflammation and proliferation. The expression of GATA6 in reprogrammed cells is attenuated, leading to an increase in sonic hedgehog signaling and FOXP1 expression, which ameliorates cellular hallmarks of aging. GATA6 is identified as a critical regulator of aging-related activities in MSCs, controlling the downstream pathways to modulate cellular senescence.
Article
Cell & Tissue Engineering
Stephen T. Moore, Takashi Nakamura, Jing Nie, Alexander J. Solivais, Isabel Aristizabal-Ramirez, Yoshitomo Ueda, Mayakannan Manikandan, V. Shweta Reddy, Daniel R. Romano, John R. Hoffman, Benjamin J. Perrin, Rick F. Nelson, Gregory I. Frolenkov, Susana M. Chuva de Sousa Lopes, Eri Hashino
Summary: Using 3D cultures of human pluripotent stem cells, researchers replicated the differentiation process of cochlear hair cells by modulating Sonic Hedgehog and WNT signaling. The resulting cells displayed characteristics consistent with both outer and inner hair cells in the cochlea. These findings suggest the development of a promising system to model the human auditory organ.
Review
Toxicology
Atsushi Masui, Toyohiro Hirai, Shimpei Gotoh
Summary: The absence of in vitro platforms for human pulmonary toxicology studies is a growing concern. Recent advances in culture methods, specifically human pluripotent stem cell-derived lung epithelial organoid culture systems, have provided potential solutions. These advancements have allowed for the in vitro recapitulation of genetic lung diseases and the evaluation of the effectiveness and toxicity of therapeutic agents, bridging the gap between bench and bedside.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Biochemical Research Methods
Jisun Kim, Jeha Jeon, Bin Song, Nayeon Lee, Sanghyeok Ko, Young Cha, Pierre Leblanc, Hyemyung Seo, Kwang-Soo Kim
Summary: By culturing human pluripotent stem cells in a physically confined area ('spot') and efficiently differentiating them into a highly enriched population of healthy and functional midbrain dopamine progenitors and midbrain dopamine neurons, a new potential for disease treatment and stem cell research is opened up.
Article
Chemistry, Multidisciplinary
Fang Liu, Zhun Liu, Weisheng Cheng, Qingquan Zhao, Xinyu Zhang, He Zhang, Miao Yu, He Xu, Yichen Gao, Qianrui Jiang, Guojun Shi, Likun Wang, Shanshan Gu, Jia Wang, Nan Cao, Zhongyan Chen
Summary: This study reveals the importance of protein quality control in cardiac development and demonstrates how the PERK-ATF4-WARS1 axis is activated to cope with differentiation-induced proteotoxic stress in human pluripotent stem cells.
Article
Cell Biology
James A. Heslop, Behshad Pournasr, Jui-Tung Liu, Stephen A. Duncan
Summary: This study reveals the importance of transcriptional regulator GATA6 in establishing the endoderm enhancer network by regulating chromatin accessibility and histone modifications. It also identifies chromatin-modifying complexes that interact with GATA6, defining the potential mechanisms by which GATA6 modulates chromatin architecture in cell-fate decisions during formative development.
Article
Biochemistry & Molecular Biology
Anna Demchenko, Ekaterina Kondrateva, Vyacheslav Tabakov, Anna Efremova, Diana Salikhova, Tatiana Bukharova, Dmitry Goldshtein, Maxim Balyasin, Natalia Bulatenko, Elena Amelina, Alexander Lavrov, Svetlana Smirnikhina
Summary: Airway and lung organoids derived from hiPSCs are valuable models for drug screening, cell-cell interaction studies, and lung disease research. This article provides a step-by-step protocol for differentiating hiPSCs into airway and lung organoids and analyzes the cellular composition and functionality of these organoids. The results demonstrate the feasibility of generating and characterizing airway and lung organoids from hiPSCs, which have diverse applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Amita Tiyaboonchai, Fabian L. Cardenas-Diaz, Lei Ying, Jean Ann Maguire, Xiuli Sim, Chintan Jobaliya, Alyssa L. Gagne, Siddharth Kishore, Diana E. Stanescu, Nkecha Hughes, Diva D. De Leon, Deborah L. French, Paul Gadue
Article
Cell Biology
Xin Cheng, Amita Tiyaboonchai, Paul Gadue
CURRENT OPINION IN CELL BIOLOGY
(2013)
Article
Developmental Biology
Jun Cheng, Amita Tiyaboonchai, Yukiko M. Yamashita, Alan J. Hunt
Article
Cell Biology
Viktoria Salzmann, Cuie Chen, C. -Y. Ason Chiang, Amita Tiyaboonchai, Michael Mayer, Yukiko M. Yamashita
MOLECULAR BIOLOGY OF THE CELL
(2014)
Article
Cell & Tissue Engineering
Amita Tiyaboonchai, Helen Mac, Razveen Shamsedeen, Jason A. Mills, Siddarth Kishore, Deborah L. French, Paul Gadue
STEM CELL RESEARCH
(2014)
Article
Biotechnology & Applied Microbiology
Qing-Shuo Zhang, Amita Tiyaboonchai, Sean Nygaard, Kevin Baradar, Angela Major, Niveditha Balaji, Markus Grompe
Summary: Enhancing the efficiency of gene repair by homologous recombination in the liver can be achieved through CRISP/Cas9 incision near the mutation site. Interventions to further improve in vivo hepatocyte gene repair include inducing cell division with thyroid hormone T3 in neonatal mice and investigating the importance of the Fanconi anemia DNA repair pathway in hepatocyte gene repair.
HUMAN GENE THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Dhwanil A. Dalwadi, Andrea Calabria, Amita Tiyaboonchai, Jeffrey Posey, Willscott E. Naugler, Eugenio Montini, Markus Grompe
Summary: Research found a high frequency of chromosomal integrations of recombinant adeno-associated viral vectors in liver cells, with most inserted sequences heavily rearranged and accompanied by deletions of host genomic sequences at integration sites. This indicates a certain risk associated with rAAV integration.
Article
Cell Biology
Anne Vonada, Amita Tiyaboonchai, Sean Nygaard, Jeffrey Posey, Alexander Mack Peters, Shelley R. Winn, Alessio Cantore, Luigi Naldini, Cary O. Harding, Markus Grompe
Summary: The study demonstrates that therapeutic modified hepatocytes can be efficiently selected in preclinical models by using mildly hepatotoxic acetaminophen, leading to significant expansion of transgene-bearing hepatocytes and reaching therapeutic thresholds in genetic liver diseases.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Amita Tiyaboonchai, Anne Vonada, Jeffrey Posey, Carl Pelz, Leslie Wakefield, Markus Grompe
Summary: This study uses self-cleaving gRNAs to create drug-selectable gene editing events in specific hepatocyte loci, resulting in expanded gene-edited cells and therapeutic levels of gene expression.
NATURE COMMUNICATIONS
(2022)
Article
Developmental Biology
Amita Tiyaboonchai, Leslie Wakefield, Anne Vonada, Catherine L. May, Craig Dorrell, David Enicks, Anusha Sairavi, Klaus H. Kaestner, Markus Grompe
Summary: The current gold-standard for genetic lineage tracing in transgenic mice is based on cell-type specific expression of Cre recombinase. As an alternative, we developed a cell-type specific CRISPR/spCas9 system for lineage tracing. This method relies on RNA polymerase II promoter driven self-cleaving guide RNAs (scgRNA) to achieve tissue-specificity. The use of RNA polymerase II promoter driven scgRNAs enables the use of CRISPR/spCas9 for genetic lineage tracing.
DEVELOPMENTAL BIOLOGY
(2023)