Article
Multidisciplinary Sciences
Simone Agostini, Elisabetta Bolognesi, Roberta Mancuso, Ivana Marventano, Lorenzo Agostino Citterio, Franca Rosa Guerini, Mario Clerici
Summary: The concentration of specific miRNAs (miR-27b-3p, miR-181a-5p, and miR-23a-3p) was found to be associated with a SNAP-25 polymorphism. In vitro experiments confirmed that miR-181a-5p and miR-23a-3p regulate SNAP-25 gene and protein expression, while miR-27b-3p does not. These findings suggest the potential use of miR-181a-5p and miR-23a-3p as biomarkers or therapeutic targets for diseases involving altered SNAP-25 expression.
Article
Oncology
Sofia Elena Tesolato, Daniel Gonzalez-Gamo, Ana Barabash, Paula Claver, Sofia Cristina de la Serna, Inmaculada Dominguez-Serrano, Jana Dziakova, Carmen de Juan, Antonio Jose Torres, Pilar Iniesta
Summary: This study aims to analyze and correlate the expression levels of four miRNAs associated with cancer and obesity in colorectal cancer (CRC) patients and a control group. The results indicate the significance of hsa-miR-143-3p and hsa-miR-181a-5p in the clinical management of CRC.
Article
Cell Biology
Etsu Tashiro, Yumi Nagasawa, Susumu Itoh, Masaya Imoto
Summary: Insulin resistance is closely related to metabolic disorders, primarily due to elevated plasma free fatty acid levels. Palmitic acid-induced increase in miRNAs suppresses insulin signaling, leading to insulin resistance.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Erica Costantini, Bruna Sinjari, Pamela Di Giovanni, Lisa Aielli, Sergio Caputi, Raffaella Muraro, Giovanna Murmura, Marcella Reale
Summary: This study aimed to evaluate the involvement of selected miRNAs in different stages of periodontitis and their relationship with the levels of inflammatory mediators in gingival crevicular fluid (GCF). The results showed that miRNA-103a-3p, miRNA-23a-3p, miRNA-15a-5p, and miRNA-223-3p were significantly upregulated in periodontitis patients, and miRNA-23a-3p, miRNA-103a-3p, and miRNA-423-5p levels differed significantly according to disease stages.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Hui Liu, Hui Guan, Xintong Tan, Yang Jiang, Feng Li, Dongxiao Sun-Waterhouse, Dapeng Li
Summary: This study investigated the interactive effects of quercetin and EGCG in anti-diabetic/anti-obesity actions. The results showed that the combination of quercetin and EGCG had stronger effects in reducing insulin resistance and hepatic gluconeogenesis compared to individual treatments. The study also identified miR-27a-3p and miR-96-5p as key regulators in the protective effect of quercetin-EGCG combination against insulin resistance. This is the first report on the contributions of these microRNAs to the synergistic effect of quercetin and EGCG.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Mai Thi Nguyen, Kyung-Ho Min, Wan Lee
Summary: This study reveals that excessive intake of saturated fatty acids (SFA) can lead to obesity and insulin resistance. The induction of miR-183-5p by SFA impairs insulin signaling, leading to the development of hepatic insulin resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ze Long, Pengcheng Dou, Weiliang Cai, Minzhi Mao, Ren Wu
Summary: High-throughput miRNA sequencing of osteoporosis from the GEO database identified miR-181a-5p as a differentially expressed microRNA. Modulation of miR-181a-5p levels in MC3T3-E1 cells affected osteogenic differentiation. Target gene prediction revealed BMP3 as a target of miR-181a-5p. Reduced expression of miR-181a-5p was validated in osteoporotic patients, implicating its importance in disease prevention.
Article
Cell Biology
Maristella Canovai, Monica Evangelista, Alberto Mercatanti, Romina D'Aurizio, Letizia Pitto, Francesca Marrocolo, Valentina Casieri, Marco Pellegrini, Vincenzo Lionetti, Sergio Bracarda, Milena Rizzo
Summary: In this study, we found that miR-96-5p, miR-183-5p, and miR-210-3p released by DCT resistant PCa clones decrease the efficacy of DCT in PCa cells. These miRNAs were found to interact with genes including FOXO1, IGFBP3, PDCD4, PPP2CB, and INSIG1, affecting the sensitivity of DCT. Additionally, circulating miR-183-5p and miR-21-5p were identified as potential biomarkers for predicting DCT resistance in PCa patients.
CELL DEATH DISCOVERY
(2023)
Article
Biochemistry & Molecular Biology
Daniel G. Henriques, Renan Lyra Miranda, Romulo Sperduto Dezonne, Luiz Eduardo Wildemberg, Aline Helen da Silva Camacho, Leila Chimelli, Leandro Kasuki, Elisa B. Lamback, Alexandro Guterres, Monica R. Gadelha
Summary: This study identified miRNAs associated with resistance to fg-SRLs treatment in acromegaly using miRNA sequencing and bioinformatic analysis. It was found that miR-181a-5p, miR-181b-5p, and miR-383-5p were differentially expressed, with miR-181a-5p and miR-181b-5p downregulated and miR-383-5p upregulated in the uncontrolled group. ROC curve analysis showed that miR-383-5p had an NPV of 84.3% and a PPV of 84.5%. Thus, these miRNAs can serve as biomarkers and tools to guide clinical decisions in response to fg-SRLs treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Wojciech Gierlikowski, Katarzyna Broniarek, Lukasz Cheda, Zbigniew Rogulski, Marta Kotlarek-Lysakowska
Summary: In the context of papillary thyroid carcinoma, miR-181a-5p has been found to directly regulate the expression of SLC5A5, potentially reducing the efficacy of radioiodine treatment. This suggests that miR-181a-5p could serve as a promising target to enhance the efficacy of radioactive iodine therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Junxin Li, Jing Shen, Yueshui Zhao, Fukuan Du, Mingxing Li, Xu Wu, Yu Chen, Shurong Wang, Zhangang Xiao, Zhigui Wu
Summary: miR-181a-5p is an important miRNA that can post-transcriptionally suppress targeted genes and is associated with various diseases. It plays a regulatory role in different types of cancer, potentially serving as a diagnostic and prognostic biomarker and influencing chemoresistance.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jianjie Li, Xiaoyue Xu, Chunhui Liu, Xiaoxue Xi, Yang Wang, Xiaotang Wu, Hua Li
Summary: miR-181a-2-3p promotes GC cell progression by targeting MYLK and enhancing tumor growth. The promoting effect of miR-181a-2-3p on GC cells is reversed when miR-181a-2-3p and MYLK are simultaneously overexpressed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Oncology
Yuanjun Lu, Yau-Tuen Chan, Hor-Yue Tan, Cheng Zhang, Wei Guo, Yu Xu, Rakesh Sharma, Zhe-Sheng Chen, Yi-Chao Zheng, Ning Wang, Yibin Feng
Summary: Our study reveals that the ETS1/miR-23a-3p/ACSL4 axis contributes to sorafenib resistance in HCC by regulating ferroptosis. This suggests that miR-23a-3p could be a potential target for improving sorafenib responsiveness in HCC patients.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Haihong Hu, Yu Wang, Zhiyuan Qin, Wen Sun, Yanhong Chen, Jiaqi Wang, Yingying Wang, Jing Nie, Lu Chen, Sheng Cai, Lushan Yu, Su Zeng
Summary: In this study, miR-4524-5p was found to downregulate the expression of MRP4, and circHIPK3 was shown to regulate MRP4 expression by sponging miR-124-3p and miR-4524-5p for the first time. These results may offer new insights into preventing MRP4-related proliferation and multiple drug resistance in HCC.
Article
Gastroenterology & Hepatology
Victor Lopez-Lopez, Pedro Jose Gil-Vazquez, David Ferreras, Ahmad H. M. Nassar, Virinder K. Bansal, Baki Topal, Jie-Gao Zhu, Shu-Hung Chuang, Rosa Jorba, Mohamed Bekheit, David Martinez-Cecilia, Pablo Parra-Membrives, Georgios Sgourakis, Anne Mattila, Aldo Bove, Silvia Quaresima, Javier Ernesto Barreras Gonzalez, Anil Sharma, Juan Jose Ruiz, Francisco Sanchez-Bueno, Ricardo Robles-Campos, Alberto Martinez-Isla
Summary: This study investigated the current status of laparoscopic management of common bile duct stones with gallbladder in situ (LBDE). The results showed that LBDE is a safe and effective approach when performed by experienced teams. However, the current training for LBDE was considered poor or very poor, indicating a need for the development of training programs for its wider application.
JOURNAL OF HEPATO-BILIARY-PANCREATIC SCIENCES
(2022)
Review
Food Science & Technology
Ricard Bou, Paola Navarro-Vozmediano, Ruben Dominguez, Miguel Lopez-Gomez, Montserrat Pinent, Albert Ribas-Agusti, Jose J. Benedito, Jose M. Lorenzo, Ximena Terra, Jose Garcia-Perez, Mirian Pateiro, Jose A. Herrera-Cervera, Rosa Jorba-Martin
Summary: The current demand for healthy and sustainable food products has led to the search for alternative sources of plant protein isolates and concentrates. Legumes are considered to be an excellent non-animal protein source due to their high protein content, adaptability to various ecological conditions, and sustainability. However, their use in human food is limited by the presence of anti-nutritional and anti-technological factors. Emerging technologies can help remove these unwanted compounds and improve the purity and yield of protein isolates.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Medicine, General & Internal
Domingo Orozco-Beltran, Juan Francisco Merino-Torres, Antonio Perez, Ana M. Cebrian-Cuenca, Ignacio Parraga-Martinez, Luis Avila-Lachica, Gemma Rojo-Martinez, Francisco J. Pomares-Gomez, Fernando Alvarez-Guisasola, Manuel Sanchez-Molla, Felix Gutierrez, Francisco J. Ortega, Manel Mata-Cases, Enrique Carretero-Anibarro, Josep Maria Vilaseca, Jose A. Quesada
Summary: The purpose of this study was to identify the factors associated with hospital admission in people over 50 years infected with SARS-CoV-2 and to assess the impact of diabetes mellitus. The results showed that age, gender, symptoms, and comorbidities were associated with hospital admission, while diabetes did not increase the risk.
JOURNAL OF CLINICAL MEDICINE
(2022)
Letter
Endocrinology & Metabolism
Eduardo Mayoral, Reyes Rave, Pablo Rodriguez de Vera, Gemma Rojo-Martinez, Gabriel Olveira, Manuel Aguilar-Diosdado, Antonio Leon-Justel, Maria Asuncion Martinez-Brocca
BMJ OPEN DIABETES RESEARCH & CARE
(2022)
Article
Agriculture, Multidisciplinary
Alba Miguens-Gomez, Marta Sierra-Cruz, Helena Segu, Raul Beltran-Debon, Esther Rodriguez-Gallego, Ximena Terra, Maria Teresa Blay, Anna Maria Perez-Vendrell, Montserrat Pinent, Anna Ardevol
Summary: This study found that acutely ingesting insect Alphitobius diaperinus protein increases food intake in rats and modifies the ex vivo enterohormone secretory profile differently than beef or almond proteins. However, these effects cannot be maintained for a longer period. The results suggest that consuming insect protein increases food intake by reducing ghrelin secretion in the small intestine.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Review
Oncology
Mihai-Calin Pavel, Raquel Casanova, Laia Estalella, Robert Memba, Erik Llacer-Millan, Elisabet Julia, Sandra Merino, Justin Geoghegan, Rosa Jorba
Summary: This study assessed the impact of neoadjuvant chemotherapy (NeoChem) on liver regeneration (LivReg). The results showed that NeoChem did not have a negative impact on LivReg, and the addition of bevacizumab to the treatment regimen was associated with better liver regeneration rates compared to standard NeoChem.
Review
Biochemistry & Molecular Biology
Mihai-Calin Pavel, Elena Ramirez-Maldonado, Eva Pueyo-Periz, Robert Memba, Sandra Merino, Justin Geoghegan, Rosa Jorba
Summary: There is a clear association between the molecular profile of colorectal cancer liver metastases and patient survival. However, much of our knowledge comes from experimental studies with limited clinical application. This article reviews the current advances in understanding the molecular behavior of colorectal cancer liver metastases and presents future therapeutic applications, focusing on dissemination of malignant cells, chemotherapy response, and molecular analysis for predicting early progression and recurrence patterns.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Mariona Jove, Natalia Mota-Martorell, Elia Obis, Joaquim Sol, Meritxell Martin-Gari, Isidre Ferrer, Manuel Portero-Otin, Reinald Pamplona
Summary: The human brain is highly susceptible to oxidative stress due to its characteristics, but neurons have evolved various adaptive mechanisms to combat oxidative stress and preserve their functionality.
Review
Biochemistry & Molecular Biology
Mariona Jove, Natalia Mota-Martorell, Elia Obis, Joaquim Sol, Meritxell Martin-Gari, Isidre Ferrer, Manuel Portero-Otin, Reinald Pamplona
Summary: The brain is one of the richest tissues in terms of lipid content and diversity in the human body, making it highly susceptible to oxidative stress. Glycerophospholipids, especially ether lipids, play a crucial role in the composition and functionality of the human brain. This article explores the involvement of ether lipids in the pathogenesis of sporadic Alzheimer's disease (sAD) and discusses their implications in neural cell physiology and antioxidant defense mechanisms.
Review
Cell Biology
Manuel Portero-Otin, M. Pia de la Maza, Jaime Uribarri
Summary: Insulin resistance is commonly observed during aging and is a major factor in chronic nontransmissible diseases. Diet, particularly the intake of advanced glycation end products (AGEs), plays a significant role in causing insulin resistance. Clinical trials and animal studies suggest that reducing dietary AGE intake can improve insulin resistance.
Article
Endocrinology & Metabolism
Enrique Ozcariz, Montse Guardiola, Nuria Amigo, Gemma Rojo-Martinez, Sergio Valdes, Pere Rehues, Lluis Masana, Josep Ribalta
Summary: The aim of this study was to combine nuclear magnetic resonance-based metabolomics and machine learning to identify a glucose-independent molecular signature associated with future type 2 diabetes development. A metabolomic analysis was performed on serum samples from individuals in the Di@bet.es study, revealing lipoprotein and glycoprotein profiles as well as 15 low molecular weight metabolites that were related to the development of type 2 diabetes. Machine learning models also highlighted the roles of inflammation and muscle as independent factors in the development of type 2 diabetes.
DIABETES RESEARCH AND CLINICAL PRACTICE
(2023)
Article
Biochemistry & Molecular Biology
M. Kathryn Brewer, Pascual Torres, Victoria Ayala, Manuel Portero-Otin, Reinald Pamplona, Pol Andres-Benito, Isidro Ferrer, Joan J. Guinovart, Jordi Duran
Summary: Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterized by motor neuron loss in the spinal cord. Glycogen accumulation in reactive astrocytes has been found to contribute to neurotoxicity and disease progression in ALS. This study demonstrates that glycogen accumulates in the spinal cord and brainstem of ALS mice and is associated with reactive astrocytes. The reduced glycogen synthesis in ALS mice leads to a longer lifespan and lower levels of pro-inflammatory cytokine Cxcl10, suggesting that glycogen accumulation is linked to inflammation and disease progression.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Nutrition & Dietetics
Jose C. E. Serrano, Eva Castro-Boque, Alicia Garcia-Carrasco, Maria Ines Moran-Valero, Daniel Gonzalez-Hedstrom, Marcelino Bermudez-Lopez, Jose Manuel Valdivielso, Alberto E. Espinel, Manuel Portero-Otin
Summary: This study validates the antihypertensive effects of an optimized extract of aged black garlic with low doses of S-allyl-cysteine (SAC) in a Grade I hypertensive population. The results show a reduction in blood pressure, an increase in nitric oxide and antioxidant capacity, and a decrease in uric acid levels and ACE activity. No changes in endothelial function and inflammatory cytokines were observed.
Article
Surgery
Rosa Jorba-Martin, Mihai C. Pavel, Laia Estalella, Erik Llacer-Millan, Elisabet Julia, Elena Ramirez-Maldonado, Eva Pueyo, Justin Geoghegan, Robert Memba
Summary: The left lateral approach robotic spleen-preserving distal pancreatectomy is a feasible and safe procedure for distal benign or small low-grade malignant tumors of the left pancreas. This surgical technique uses the da Vinci robotic system to overcome technical challenges and reduce operative risks by changing the surgical position and preserving the splenic vessels.
JOURNAL OF ROBOTIC SURGERY
(2023)
Article
Oncology
Antonio Altuna-Coy, Xavier Ruiz-Plazas, Veronica Arreaza-Gil, Jose Segarra-Tomas, Matilde R. Chacon
Summary: In this study, downregulated exomiRNAs were identified in prostate cancer cells, and their potential roles in the diagnosis and prognosis of prostate cancer were explored. The altered pathways and genes related to miRNA biosynthesis, miRNA-mediated gene silencing, and cell proliferation were identified. The gene expression profiles of TNRC6B, CDK6, and AGO1 were found to be deregulated in prostate cancer tissue and could improve the prognostic power of classical biomarkers.
CANCER CELL INTERNATIONAL
(2023)