Article
Chemistry, Physical
Shiyong Teng, Yandong Han, Yang Hu, Jinshuo Li, Min Wang, Zilong Guo, Wensheng Yang
Summary: In this study, we successfully prepared HPMO capsules with tunable shell thickness and efficiently loaded hydrophobic drug PTX into the capsules. The prepared capsules exhibited excellent swellability and improved performance in killing cancer cells.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Majid Vafaeezadeh, Kristin Weber, Anna Demchenko, Philipp Losch, Paul Breuninger, Andrea Losch, Michael Kopnarski, Sergiy Antonyuk, Wolfgang Kleist, Werner R. Thiel
Summary: A new method for synthesizing a Janus periodic mesoporous organosilica material (JPMO) is introduced, where the material is capable of forming stable Pickering emulsions. This bifunctional material may find widespread applications in various fields related to material science and catalysis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Engineering, Chemical
Qi Zhang, Siru Ren, Song Xue, Ang Li, Siyao Liu, Xiaodong Sun
Summary: The study evaluated six novel ionic liquids as chiral selectors in capillary electrophoresis, showing better enantioseparation performance compared to traditional chiral selectors.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Engineering, Chemical
Qi Zhang, Siru Ren, Ang Li, Jing Zhang, Song Xue, Xiaodong Sun
Summary: This study designed and successfully synthesized six tartaric acid-based IL-type chiral selectors, which demonstrated good enantioseparation performance in chiral capillary electrophoresis. Among them, [BMPIP](2)-L-TT showed the best performance, indicating the significant role of cations in enantiorecognition process.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Jan Bilek, Dusan Koval, Petra Sazelova, Veronika Solinova, Lukas Severa, Paul E. Reyes Gutierrez, Filip Teply, Vaclav Kasicka
Summary: A chiral capillary electrophoresis (CE) method has been developed to separate the P- and M-enantiomers of 11 new diquats, achieving fast and efficient separations within 5-7 minutes. The method utilizes acidic buffer and sulfated cyclodextrins as chiral selectors, with sulfated γ-cyclodextrin showing the best selectivity by baseline separating all 11 diquats.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemical Research Methods
Li Qi, Juan Qiao
Summary: Chiral ligand exchange capillary electrophoresis (CLE-CE) has gained significant interest in chiral separation science due to its simplicity, tunable migration order, and outstanding performance. However, challenges such as limited chiral ligand species, unclear mechanism of enantioseparation, and unsatisfactory enantioseparation efficiency restrict its applications. Designing desirable chiral ligand species and exploring profitable central metal ions are efficient strategies to overcome these obstacles and improve the performance and application of CLE-CE.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Biochemical Research Methods
Paola Peluso, Bezhan Chankvetadze
Summary: Cyclodextrins (CDs) are versatile cyclic oligosaccharides obtained from starch degradation, with applications in various industries due to their hydrophilic and hydrophobic regions. Their inherent chirality allows for successful use as chiral selectors, while multidisciplinary approaches have shed light on recognition mechanisms. Computational techniques have become useful in modeling molecular dynamics, binding energies, and noncovalent interactions between CD and enantiomers.
Review
Biochemistry & Molecular Biology
Sami El Deeb, Camilla Fonseca Silva, Clebio Soares Nascimento Junior, Rasha Sayed Hanafi, Keyller Bastos Borges
Summary: This review article summarizes the literature on chiral capillary electrokinetic chromatography from January 2016 to March 2021, focusing on the separation of drugs and other chiral molecules. It discusses measures to enhance detection sensitivity, chiral recognition techniques, as well as the application of Quality by Design principles and molecular modeling.
Article
Chemistry, Analytical
Chengchen Zhang, Xiaofei Ma
Summary: A synergistic system for enantioseparation in capillary electrophoresis (CE) with a chiral ionic liquid (CIL) based on D-10-camphorsulfonic acid as an additive and carboxymethyl-beta-cyclodextrin (CM-beta-CD) as the chiral selector is presented in this work. The proposed method showed excellent enantioseparation performance towards sixteen chiral drugs, with notably improved resolution (Rs) and selectivity factor (alpha) compared to the single CM-beta-CD system. Several key parameters were investigated, and Statistical Product and Service Solutions (SPSS) was used to prove the potential synergistic effect. The nuclear magnetic resonance (NMR) results further demonstrated the function of the CIL and the superiority of the synergistic system. Chiral impurity determination of chlorpheniramine maleate sample was successfully carried out using the established method.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Electrochemistry
Yuefang Hu, Yuxin Chen, Liyun Lin, Guohua Liu, Zhengguo Wang, Dongmei Liang
Summary: A simple and flexible capillary electrophoresis with electrochemiluminescence (CE-ECL) detection method was developed for simultaneous enantioseparation of three β-blocker drugs. By optimizing buffer conditions, the separation efficiency and detection sensitivity were improved, achieving successful analysis of enantiomers in urine samples with high recoveries and stability.
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
(2021)
Article
Chemistry, Inorganic & Nuclear
Wanlu Liu, Anna M. Kaczmarek, Pascal Van der Voort, Rik Van Deun
Summary: In this study, three unique organic-inorganic hybrid nanocomposite materials were developed for metal ion and solvent sensing. The results demonstrate the potential application of these materials in environmental and chemical industries.
DALTON TRANSACTIONS
(2022)
Article
Chemistry, Analytical
Jumi Kim, Hyunwoo Kim
Summary: In this study, a comprehensive chiral analysis method based on F-19 NMR spectroscopy was introduced for accurate differentiation and analysis of monosaccharides. The method utilizes sugar hydrazones with fluorine labels and chiral metal complexes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mahsa Javan, Behrouz Seyfinejad, Elaheh Rahimpour, Vahid Jouyban-Gharamaleki, Raha Kaviani, Ali Shayanfar, Mojtaba Varshochi, Maryam Khoubnasabjafari, Abolghasem Jouyban
Summary: Breath analysis is an effective method for monitoring systemic or respiratory ailments. This study proposes a simple chiral capillary electrophoresis method for quantifying enantiomers of ofloxacin in exhaled breath condensate using an online field-amplified sample injection stacking method. The method has been validated and proven to be specific for routine analysis of ofloxacin's enantiomers. It offers a convenient and reliable way to monitor the levels of ofloxacin's enantiomers in patients' breath samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Materials Science, Multidisciplinary
Chunhui Liu, Anna M. Kaczmarek, Himanshu Sekhar Jena, Zetian Yang, Dirk Poelman, Pascal van der Voort
Summary: Recent research in lanthanide materials has contributed to the development of chemical sensors, but the current sensors still have limited detection range. We developed lanthanide-based hollow spheres for luminescence turn-off sensing of Hg2+ with low detection limit and wide detection range. This hybrid material exhibited both downshifting and upconversion luminescence.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
Review
Chemistry, Multidisciplinary
Majid Vafaeezadeh, Werner R. Thiel
Summary: This article provides an overview of the broad applications of periodic mesoporous organosilicas (PMOs) in various research fields such as chemistry and material science. By choosing suitable organic groups, the surface properties of PMOs can be tuned. The review also discusses four leading areas of PMO nanomaterials and their potential applications for future developments.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Applied
Sonia Morante-Zarcero, Alba Endrino, Natalia Casado, Damian Perez-Quintanilla, Isabel Sierra
Summary: In this study, mesostructured silicas with wormhole-like pore arrangement were synthesized and evaluated as carriers for bioactive flavonoids. The results showed that these silicas can effectively load and release quercetin and naringin, with potential applications in food and biomedical fields. Furthermore, the antioxidant activity of the encapsulates was determined, confirming the suitability of wormhole-like mesostructured silicas as carriers for enhancing stability and bioavailability of flavonoids.
JOURNAL OF POROUS MATERIALS
(2022)
Article
Biochemical Research Methods
Maria Angeles Garcia, Sara Jimenez-Jimenez, Maria Luisa Marina
Summary: This work developed an Electrokinetic Chromatography (EKC) method that successfully separated the four stereoisomers of the acetamide herbicide dimethenamid. Different anionic cyclodextrins (CDs) were screened, and the use of a dual CD system improved the chiral separation. The addition of deep eutectic solvents showed beneficial effects on the separation in terms of resolution.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Chemistry, Analytical
Judith Ganan, Gonzalo Martinez-Garcia, Sonia Morante-Zarcero, Damian Perez-Quintanilla, Isabel Sierra
Summary: Alkaloids are widely found secondary metabolites in plants, many of which have significant biological activity and pharmacological applications. However, the potential adverse effects of alkaloids present in food, such as acute hepatotoxicity, genotoxicity, and neurological damage, raise concerns among consumers. Therefore, developing sensitive, selective, and convenient sensors for alkaloids screening is crucial. Electrochemical detection platforms, particularly those based on nanomaterials, have shown great potential as a cost-effective, efficient, eco-friendly, and easy-to-operate analytical approach for alkaloids detection in various applications.
MICROCHEMICAL JOURNAL
(2023)
Review
Chemistry, Multidisciplinary
Natalia Casado, Gema Casado-Hidalgo, Lorena Gonzalez-Gomez, Sonia Morante-Zarcero, Isabel Sierra
Summary: Pyrrolizidine alkaloids, tropane alkaloids, and opium alkaloids are natural plant toxins that are of great concern in food safety due to their presence in various foods and feeds. Despite recent legislation, the concentration levels of these toxins often exceed the maximum limits established by authorities. Moreover, the regulations primarily focus on raw materials and do not consider processed products. Therefore, it is crucial to assess the health risk associated with these alkaloids through diet. This review aims to provide insight into these alkaloids and their influence on content and stability during food processing and culinary preparation, highlighting the need for further research to understand their transformation and determine the exact risk exposure to the population.
APPLIED SCIENCES-BASEL
(2023)
Article
Food Science & Technology
Sergio Izcara, Rosa Perestrelo, Sonia Morante-Zarcero, Isabel Sierra, Jose S. Camara
Summary: Edible flowers have gained popularity in gourmet cuisine for their attractive colors, freshness, texture, and aromatic notes. They not only enhance the visual appeal of dishes but also contain phytochemical compounds that are beneficial to human health. This study analyzed the volatile metabolites of four different species of edible flowers used in gourmet dishes, revealing their chemical profiles and potential applications in various industries. The findings provide insights into the distinctive characteristics and bioactive properties of edible flowers.
Article
Biochemical Research Methods
Sandra Salido-Fortuna, Chiara Dal Bosco, Alessandra Gentili, Maria Castro-Puyana, Maria Luisa Marina, Giovanni D'Orazio, Salvatore Fanali
Summary: In this study, the enantioseparation of 10 drugs from different families was carried out using nano-LC and a column with immobilized amylose tris(3-chloro-5-methylphenylcarbamate) silica. The effect of adding different salts to the mobile phase was evaluated for chiral separation. Buffer concentration in the mobile phase and liquid-liquid microextraction using isoamyl acetate were studied to improve the separation and increase sensitivity, achieving satisfactory recoveries (>70%) for four chiral drugs from tap and environmental waters.
Article
Food Science & Technology
Lorena Gonzalez-Gomez, Judith Ganan, Sonia Morante-Zarcero, Damian Perez-Quintanilla, Isabel Sierra
Summary: This study investigated the occurrence of atropine and scopolamine in 51 commercial spices purchased in Portugal and Spain. More than half of the samples (67%) were contaminated with one or both analytes. Fennel, cloves, and coriander showed the highest concentrations.
Article
Biochemistry & Molecular Biology
Samuel Bernardo-Bermejo, Elena Sanchez-Lopez, Maria Castro-Puyana, Ana B. Fernandez-Martinez, Francisco Javier Lucio-Cazana, Maria Luisa Marina
Summary: Apoptotic bodies (ABs), a type of extracellular vesicles, play a significant role in the pathogenesis of various diseases. This study used a non-targeted metabolomic approach to investigate the impact of different apoptotic stimuli (cisplatin or UV light) on the metabolites involved in apoptosis propagation. The results revealed stimulus-specific differences in metabolite abundances, suggesting that these metabolites may vary in their contribution to apoptosis depending on the apoptotic stimulus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Lorena Gonzalez-Gomez, Sonia Morante-Zarcero, Jorge A. M. Pereira, Jose S. Camara, Isabel Sierra
Summary: This study analyzed 33 samples of tea and herbal tea infusions purchased in Spain and Portugal and found that 64% of the samples were contaminated with atropine and scopolamine. White and green teas were more contaminated compared to black and other herbal teas. Among the 21 contaminated samples, 15 had concentrations above the maximum limit set by the EU Commission Regulation (0.2 ng/mL) for liquid herbal infusions.
Review
Chemistry, Multidisciplinary
Cristina V. Berenguer, Laura Garcia-Cansino, Maria Angeles Garcia, Maria Luisa Marina, Jose S. Camara, Jorge A. M. Pereira
Summary: The increasing demand for food, climate changes, global warming, and arable land erosion pose great pressure on the food chain, especially fresh fruits and vegetables. In order to increase productivity and shelf life, safety precautions are often overused, causing concerns among consumers and public authorities. This review discusses the potential of microextraction as a strategy to improve food safety requirements by comparing it to conventional extraction approaches. It highlights the advantages of microextraction techniques, such as simpler and faster protocols, reduced waste, and potential for automation, and suggests their progressive substitution for conventional extraction approaches in various analytical applications.
APPLIED SCIENCES-BASEL
(2023)
Article
Food Science & Technology
Begona Fernandez-Pintor, Natalia Casado, Sonia Morante-Zarcero, Isabel Sierra
Summary: Pyrrolizidine alkaloids (PAs) have attracted attention in food safety due to their potential health risk. Tea and herbal tea, as they can be contaminated with PAs-producing weeds, have been particularly highlighted. This study simulated the cross-contamination of chamomile tea samples with weeds to evaluate the effect of brewing on PA content. The results showed that PAs are stable compounds and the initial PA concentration strongly affects their thermal stability. Not all PAs are effectively transferred from dry material to the infusion during brewing, and the extraction efficiency may be influenced by the type of weed. The study emphasizes the importance of analyzing infusion samples to correctly assess the risk exposure of consumers to PAs.
Article
Chemistry, Applied
Gema Casado-Hidalgo, Sonia Morante-Zarcero, Damian Perez-Quintanilla, Isabel Sierra
Summary: There is a current trend to add seeds such as poppy seeds in yoghurts to enhance their nutrition. However, these seeds may be contaminated with opium alkaloids (OAs) which can be harmful to health. In this study, an analytical methodology was developed to detect OAs in commercial yoghurts and showed their presence but below the limit of quantification. The study also found that lactic fermentation and storage can lead to degradation of OAs in yoghurts.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)