Article
Biochemical Research Methods
Xinhua Zhou, Xu Chen, Xiaomeng Yin, Mingyang Wang, Juanjuan Zhao, Yan Ren
Summary: In this study, a comprehensive method combining full scan (FS)-parent ions list (PIL)-higher energy collision induced dissociation (HCD)-MS/MS was used for the chemical profiling of flavonoids in Scutellaria barbata. The integration of mass defect filtering (MDF) and diagnostic product ions (DPIs) into the screening and identification strategy improved the subtype classification and structural confirmation. The study identified or tentatively characterized 127 target flavonoids and provided a novel approach for the systematic characterization of complex components in herbal medicines.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Ya-Liu Fan, Run-Zhou Liu, Qin Tan, Heng-Li Zhao, Min Song, Rui Wang, Ping Li, Hua Yang
Summary: This study proposed a comprehensive chemical profiling method for traditional Chinese medicine, utilizing a self-built component database and integrated identification strategy to quickly locate potential components and eliminate interfering ions, facilitating in-depth analysis of compounds.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Meilin Xu, Lunzhao Yi, Dabing Ren, Boyan Li
Summary: This study analyzed flavonoids in citrus fruit using ultra-high liquid chromatography coupled to high resolution mass spectrometry. A strategy combining mass defect filtering and MS/MS-based molecular networking was proposed, which effectively removed interference peaks and annotated unknown compounds in complex samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Applied
Meiyue Dong, Zhenhua Tian, Yanni Ma, Zhongyi Yang, Zhen Ma, Xiaoming Wang, Yunlun Li, Haiqiang Jiang
Summary: This study developed an integrated data filtering and identification strategy to characterize the GSLs in 25 commonly Brassicaceae tissues, successfully identifying 47 GSLs and establishing profiles for each tissue, which can be used to distinguish different Brassicaceae species.
Article
Plant Sciences
Ndamulelo Nengovhela, Dakalo T. Mbedzi, Ashwell R. Ndhlala, Lutendo M. Mathomu, Msizi I. Mhlongo, Ntakadzeni E. Madala
Summary: A comparative study was conducted on the leaves of Coccinia grandis and Coccinia rehmannii using metabolite profiling, which revealed differences in the conjugation of hydroxycinnamic acids (HCAs). The findings suggest that conjugation and isomerization processes can serve as chemotaxonomic markers and provide insights for future research on the pharmacological potency of these plants.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Chemistry, Applied
Xiao-Min Liu, Xiu-Li Xu, Xue-Mei Nie, Xue-Song Feng, Feng Zhang
Summary: A holistic strategy for discovering structural analogs using high-resolution Orbitrap mass spectrometry was established and successfully applied to detecting potential hazards in meat, providing a new method for the identification of multi-class drugs. By processing data through an integrated filtering strategy, characteristic features of homolog-type chemical substances were effectively extracted, leading to successful identification of new compounds and demonstrating significant advantages over individual filtration methods in screening and identifying complex samples.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Chemistry, Applied
Lianwu Xie, Qiachi Fu, Shuyun Shi, Jiawei Li, Xinji Zhou
Summary: A new analytical strategy was proposed to identify 33 bioactive compounds from Buddleja Flos, with six flavonoid aglycones showing excellent alpha-glucosidase inhibitory activities. The results demonstrate the high efficiency of the proposed strategy in profiling bioactive compounds from natural products.
Article
Plant Sciences
Danniela Priscylla Vasconcelos Faleiro, Rosana Casoti, Daniela Aparecida Chagas-Paula, Guillermo Federico Padilla-Gonzalez, Rejane Barbosa de Oliveira, Fernando Batista Da Costa
Summary: This study identified 3-O-E-caffeoylquinic acid and 3-O-methylquercetin in Aldama species as potential inhibitors of COX-1 and 5-LOX. Through LC-MS metabolomics and in vitro enzymatic inhibition assays, it was confirmed that these compounds could act as dual inhibitors of inflammatory enzymes, marking the first report of their role in this dual inhibition.
PHYTOCHEMISTRY LETTERS
(2021)
Article
Chemistry, Analytical
Jiayun Xue, Shun Liu, Yun Kang, Yaqin Wang, Weiyu Weng, Ping Yang, Jianming Huang
Summary: LC-MS is used to identify natural compounds, but requires complex data analysis. This study develops a data-mining strategy combining MN, MDF, and DFIF to identify phytochemicals in Stephania tetrandra. The strategy successfully identifies 144 compounds, including 11 potential new compounds and 63 compounds first found in this species. This strategy can efficiently profile chemical constituents and guide the discovery of new compounds from medicinal plants.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Yongzhe Gu, Lele Wei, Yue Liu, Yun Luo, Ting Tan
Summary: This study developed an analytical method combining high-performance liquid chromatography and mass spectrometry to successfully identify 124 compounds in Yinqiao powder, and characterized 32 of them by comparing with reference standards. This research is of great significance for discovering the active ingredients of Yinqiao powder and establishing its quality standard.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Biochemistry & Molecular Biology
Xia Xu, Ting Li, Ke Zhang, Yan Cao, Li Liu, Shilin Zhang, Pengfei Tu, Yuelin Song, Yunfang Zhao, Jun Li
Summary: This study utilized LC-MS/MS to profile the chemical composition of Epimedii Herba, comparing three original sources to demonstrate the utility of the strategy. Results showed no significant species-differences in the chemical patterns of the herb. The study proposed a robust strategy for rapid chemical profiling of herbal medicines.
Article
Biochemical Research Methods
Li Cheng, Fang Wang, Yuanxin Cao, Chaoying Tong, Qisheng Wei, Shuyun Shi, Ying Guo
Summary: To the best of our knowledge, this is the first report on the systematic investigation of antitumor polymethoxylated flavonoids (PMFs) in Citri Reticulatae Pericarpium Viride (CRPV). A method combining MCF-7 cell biospecific extraction and HPLC-QTOF-MS/MS was developed for the rapid and specific profiling of antitumor PMFs in CRPV. Sixteen antitumor PMFs were identified, with compound 15 showing excellent activity with a lower IC50 value than 5-fluorouracil. Nobiletin and tangeretin, two major PMFs, exhibited moderate antitumor activity.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Agriculture, Multidisciplinary
Chunxia Zhang, Meiyu Liu, Xiaoyan Xu, Jia Wu, Xue Li, Hongda Wang, Xiumei Gao, Dean Guo, Xiaoxuan Tian, Wenzhi Yang
Summary: This research evaluated the coverage of data-dependent acquisition (DDA) in untargeted metabolite/component identification in the food sciences and pharmaceutical analysis. Using Panax ginseng flower buds as an example, DDA on a Q-Orbitrap mass spectrometer demonstrated higher coverage compared to other MS2 acquisition approaches. By predicting a large-scale molecular library and filtering based on mass defect, new component MS2 spectra were obtained and unknown ginsenosides were characterized. In conclusion, a molecular-prediction-oriented DDA approach can enable the discovery of potentially novel molecules for functional food and drug development.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Environmental Sciences
Xianghai Wang, Zhenhua Mu, Shifu Bai, Yining Feng, Ruoxi Song
Summary: This paper proposes an MS-Pansharpening algorithm based on dual constraint Guided Filtering in the nonsubsampled shearlet transform (NSST) domain. The innovation lies in the DCGIF model, weighted information injection model, and a new MS-Pansharpening algorithm in NSST domain, which effectively improves spatial resolution of the fused MS image while maintaining its spectral characteristics.
Article
Chemistry, Multidisciplinary
Yueguang Mi, Xiangyang Wang, Meiting Jiang, Meiyu Liu, Xiaoyan Xu, Ying Hu, Hongda Wang, Feifei Yang, Jing Wang, Jie Liu, Qi Jing, Boxue Chen, Xue Li, Wenzhi Yang
Summary: This study employed PIL-DDA and DIA-MDF strategies to simultaneously investigate the metabolites of Dehydrocorynine (DHC) in rat plasma, bile, urine, and feces. A total of 40 metabolites were identified, revealing the metabolic pathways and sites of DHC. This research provides important insights into the metabolic profiles of traditional Chinese medicine components.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)