Article
Multidisciplinary Sciences
Yuriko Sobu, Paulina S. Wawro, Herschel S. Dhekne, Wondwossen M. Yeshaw, Suzanne R. Pfeffer
Summary: Mutations that activate LRRK2 protein kinase lead to Parkinson's disease and hinder cilia formation. LRRK2 kinase activity decreases the probability of ciliation and increases the percentage of cilia lost upon serum addition. Rab10 and RILPL1 proteins play crucial roles in these processes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Hyo-Jeong Yong, Jong-Ik Hwang, Jae-Young Seong
Summary: This study highlights the critical role of FAM19A1 in neuronal maturation and function by affecting dendritic spine development and neurite arborization, demonstrating its involvement in maintaining neural integrity and connectivity.
Article
Chemistry, Medicinal
Pasquale Linciano, Claudia Sorbi, Giacomo Rossino, Daniela Rossi, Andrea Marsala, Nunzio Denora, Martina Bedeschi, Noemi Marino, Giacomo Miserocchi, Giulio Dondio, Marco Peviani, Anna Tesei, Simona Collina, Silvia Franchini
Summary: In this study, novel benzylpiperidine/benzylpiperazine-based compounds were designed and tested as Sigma-1 receptor (S1R) agonists. The compounds 6b and 8b showed high affinity for S1R and selectivity over S2R and NMDAR. They exhibited neuroprotective activity and enhanced neurite outgrowth in cell lines, as well as good efficacy/safety profile in in-vitro and in-vivo models. These results encourage further preclinical investigations of compounds 6b and 8b on neurodegenerative diseases.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Plant Sciences
Jin A. Jung, Heon Joo Lee, Myoung Chong Song, Areum Hwangbo, Ji Yoon Beom, Soo Jung Lee, Dong Jin Park, Ji Hoon Oh, Sang-Jun Ha, Eunji Cheong, Yeo Joon Yoon
Summary: Separating the immunosuppressive activity of FK506 from its neurotrophic activity, two new FK506 analogues were synthesized with a proline substitution, which significantly reduced immunosuppressive activity while retaining neurotrophic activity. This replacement is critical for enhancing neurotrophic activity while reducing strong immunosuppressive activity.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Chemistry, Applied
Shane M. Hickey, Ian R. D. Johnson, Elena Dallerba, Mark J. Hackett, Massimiliano Massi, Joanna Lazniewska, Lauren A. Thurgood, Frederick M. Pfeffer, Douglas A. Brooks, Trent D. Ashton
Summary: We introduce a highly fluorescent, solvatochromic, 1,8-naphthalimide probe that enables the simultaneous imaging of lipid droplets and endoplasmic reticulum, with distinguishable emission maxima. By delineating organelles based on their emission profiles, we are able to visualize lipid droplet biogenesis and intercellular trafficking events. Furthermore, this probe demonstrates potential histochemical applications by staining myelin lipids in ex vivo brain tissue.
Article
Biochemical Research Methods
Baiying Li, Yonglun Zeng, Sze Wan Lo, Yusong Guo, Liwen Jiang
Summary: This article describes an Arabidopsis-derived in vitro vesicle formation system for the reconstitution, purification, and characterization of plant-derived COPII vesicles. The method involves the isolation of microsomes and cytosol from suspension-cultured cells, in vitro COPII vesicle reconstitution and purification, and biochemical and microscopic analysis using specific antibodies. The entire procedure takes approximately 18-20 hours of operation time and allows plant researchers without specific expertise to achieve organelle purification or vesicle reconstitution for further characterization.
Article
Biochemistry & Molecular Biology
Benhong Xu, Chuanyue Gao, Huan Zhang, Xinfeng Huang, Xifei Yang, Chen Yang, Wei Liu, Desheng Wu, Jianjun Liu
Summary: The study revealed the important roles of PRDX3 in neurite outgrowth and Alzheimer's disease (AD) development. Knockdown of PRDX3 expression promoted retinoic acid-induced neurite outgrowth and led to dysregulation of multiple proteins.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Urko Del Castillo, Rosalind Norkett, Wen Lu, Anna Serpinskaya, Vladimir Gelfand
Summary: Ataxin-2 plays a crucial role in regulating neuronal cytoskeletal dynamics and organelle trafficking, as evidenced by fruit fly models, where its loss led to defects in nervous system development and survival.
Article
Multidisciplinary Sciences
Hyunwoo Kim, Seowhang Lee, Youngsoo Jun, Changwook Lee
Summary: This study reveals the molecular mechanism and structural basis of how MIGA2 regulates ERMCS formation and facilitates lipid trafficking at ERMCS.
NATURE COMMUNICATIONS
(2022)
Review
Immunology
Jana Messias Sandes, Regina Celia Bressan Queiroz de Figueiredo
Summary: The endoplasmic reticulum (ER) is an important organelle in higher eukaryotic cells responsible for protein folding and assembly. It undergoes strict quality control to ensure proper processing of proteins, with unfolded proteins being targeted for degradation through the ER-associated degradation (ERAD) complex. Additionally, the ER responds to stress through the unfolded protein response (UPR) to restore homeostasis or induce cell death. Protozoan parasites such as Trypanosoma cruzi, Trypanosoma brucei, and Leishmania spp. also have similar ER structures and stress response mechanisms, making them attractive targets for chemotherapy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yu-pu Liu, Ming-yue Tian, Yi-duo Yang, Han Li, Tian-tian Zhao, Jin Zhu, Fang-fang Mou, Guo-hong Cui, Hai-dong Guo, Shui-jin Shao
Summary: This study found that the level of miR-21 was decreased in serum exosomes from DPN rats. Under high glucose conditions, Schwann cells (SC) proliferation was inhibited and cell apoptosis was increased. The expression of miR-21 in cells and exosomes was downregulated. miR-21 expression and SC-derived exosomes could affect neurite outgrowth through the AKT signaling pathway. This suggests the importance of SC-derived exosomal miR-21 in the regulation of neurite outgrowth under high glucose conditions.
Article
Multidisciplinary Sciences
Asako Goto, Shota Sakai, Aya Mizuike, Toshiyuki Yamaji, Kentaro Hanada
Summary: Casein kinase 1 gamma (CK1G) plays a role in regulating cellular functions and has been found to inactivate the ceramide transport protein (CERT) through phosphorylation. In this study, it was discovered that loss of the C-terminal region of CK1G3 led to hyperactivation of the kinase and redistribution within the cell. These findings suggest that CK1Gs are regulated through compartmentalization to access specific intracellular organelles.
Article
Medicine, Research & Experimental
Vikas Daggubati, Jordan Hochstelter, Anirudh Bommireddy, Abrar Choudhury, Alexis Leigh Krup, Pervinder Kaur, Pakteema Tong, Amy Li, Libin Xu, Jeremy F. Reiter, David R. Raleigh
Summary: CDK6 plays a critical role in HH-associated medulloblastoma, but resistance to single-agent therapy has been a challenge. Decreased ribosomal protein expression underlies resistance to CDK6 inhibition, leading to ER stress and activation of the unfolded protein response.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Plant Sciences
Thomas Gate, Lionel Hill, Anthony J. Miller, Dale Sanders
Summary: The study reveals that the AtIAR1 gene in Arabidopsis controls the hydrolysis of stored conjugated auxin through zinc transport, thus affecting root growth. Mutations in AtIAR1 also result in altered auxin metabolism and changes in phenotypes related to auxin activity.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Plant Sciences
Stephan Clemens
Summary: Nearly 10% of plant proteins belong to the zinc proteome and require zinc as a catalyst or structural element, with the majority of protein-bound zinc in eukaryotic cells found in the cytosol. Various metabolites and peptides serve as zinc buffers to supply zinc to enzymes, transporters, or sensor proteins, with zinc acquisition mediated by specific proteins and zinc transportation controlled by different mechanisms. Recent studies in mammalian cells have revealed the regulatory roles of zinc in modulating cellular zinc pools and influencing protein interactions and signaling cascade activities.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Neurosciences
Catherine H. H. Hor, Bor Luen Tang, Eyleen L. K. Goh
REVIEWS IN THE NEUROSCIENCES
(2018)
Review
Cell Biology
Bor Luen Tang
Letter
Cell Biology
Bor Luen Tang, Yi Shan Lim
Article
Cell Biology
Bor Luen Tang
CELL ADHESION & MIGRATION
(2010)
Review
Neurosciences
Bor Luen Tang
BRAIN RESEARCH BULLETIN
(2018)
Editorial Material
Cell Biology
Bor Luen Tang
Article
Medical Ethics
Nicole Shu Ling Yeo-Teh, Bor Luen Tang
Summary: Investigations into research misconduct are typically guided by ethics and integrity policies, but the lack of specific severity criteria can result in varying conclusions and impact the standardization of sanctions. Proposed research misconduct severity matrix aims to address this issue by considering multiple ethical elements with different weights to determine the severity of misconduct more objectively.
ACCOUNTABILITY IN RESEARCH-POLICIES AND QUALITY ASSURANCE
(2022)
Article
Neurosciences
Bor Luen Tang
Summary: The early secretory pathway is crucial for a constant supply of secretory and plasma membrane materials in mammalian cells. Neurons have a high demand for membrane dynamics and protein/lipid trafficking, and mutations in genes encoding early secretory pathway components can lead to neurodevelopmental or neurological disorders.
REVIEWS IN THE NEUROSCIENCES
(2021)
Article
Medical Laboratory Technology
Nicole Shu Ling Yeo-Teh, Bor Luen Tang
Summary: Predatory journals masquerade as genuine academic publications without rigorous peer review, focusing on financial profit over scientific dissemination. Such practices can flood scientific literature with unsound research, potentially misleading policy makers and damaging the reputation of science. Wilfully submitting to predatory journals may be considered an act of avoidance of peer review and scientific misconduct, which could be effectively discouraged through institutional and funding rules.
Review
Cell Biology
Bor Luen Tang
Summary: Cholesterol plays important structural and regulatory roles in animal cells, and is crucial for post-injury axon regrowth. Depletion and inhibition of cholesterol synthesis can enhance axon regeneration, possibly by interfering with axon growth inhibitory signals and enhancing neuronal survival signaling processes.
NEURAL REGENERATION RESEARCH
(2022)
Article
Neurosciences
Wee Meng Lim, Eunice W. M. Chin, Bor Luen Tang, Tingting Chen, Eyleen L. K. Goh
Summary: WNK3 plays a crucial role in maintaining the GABAergic inhibitory tone in mature neurons, influencing neuronal development and excitability. Its knockdown results in hyperpolarization of E-GABA in mature neurons, without affecting immature neurons, suggesting a role in the developmental switch of GABA function. This study highlights the importance of WNK3 in regulating GABA signaling and neuronal physiology.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Medical Ethics
Nicole Shu Ling Yeo-Teh, Bor Luen Tang
Summary: Good record keeping practice and research data management are essential for responsible research conduct and reproducibility of findings. Inadequate research data management often accompanies research misconduct. Research data mismanagement (RDMM) can be considered a questionable research practice and in severe cases, an act of research misconduct. This article analyzes the contexts and circumstances under which RDMM can be viewed as misrepresentation or fabrication of research data, and discusses how it can be adjudicated as research misconduct based on intent and consequences.
ACCOUNTABILITY IN RESEARCH-POLICIES AND QUALITY ASSURANCE
(2022)
Editorial Material
Cell Biology
Bor Luen Tang
Summary: This article provides an editorial overview of the papers collected in this Special Issue on the Regulation and Function of Small GTPases 2.0.
Article
Neurosciences
Felicia Yu Hsuan Teng, Bor Luen Tang
REVIEWS IN THE NEUROSCIENCES
(2010)
