Article
Biochemistry & Molecular Biology
Jen-Tai Lin, Kuo-Wang Tsai
Summary: The study showed that certain miRNAs in urine could potentially serve as noninvasive biomarkers for diagnosing bladder cancer, with implications for early detection and patient survival. Further validation in larger cohorts is necessary to confirm the clinical utility of urinary miRNAs in bladder cancer diagnosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Jinshan Xu, Shuxiong Zeng, Jun Li, Li Gao, Wenjun Le, Xin Huang, Guandan Wang, Bingdi Chen, Zhensheng Zhang, Chuanliang Xu
Summary: The study developed a novel UCT method called Nano-cytology by mixing urine with mNPs to improve the sensitivity of bladder cancer detection. The Nano-cytology assay showed significantly higher sensitivity compared to the UCT assay, and the combination of the two approaches further increased sensitivity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, General & Internal
Zuhu Yu, Chong Lu, Yongqing Lai
Summary: In this study, the expression profiles of miRNAs in serum samples of bladder cancer patients and healthy controls were detected, and five differentially expressed miRNAs were identified. A three-miRNA signature consisting of miR-27b-3p, miR-381-3p, and miR-451a was constructed with a high diagnostic efficiency for bladder cancer. This three-miRNA signature may serve as a promising non-invasive biomarker for early diagnosis of bladder cancer.
ANNALS OF MEDICINE
(2023)
Article
Biology
Jae-Hak Ahn, Chan-Koo Kang, Eun-Mee Kim, Ah-Ram Kim, Aram Kim
Summary: Bladder cancer is the fourth most common cancer in men, with most cases being non-muscle-invasive. The high recurrence rate in non-muscle-invasive bladder cancer is a critical issue. Limited urine tests hinder the effective detection of superficial and small bladder tumors. Cystoscopy, although the gold standard for diagnosis, is associated with complications. Therefore, the development of urinary biomarkers is necessary for the detection of bladder cancer.
Article
Chemistry, Analytical
Antonio Domenech-Carbo, Jose Luis Pontones, Clara Domenech-Casasus, David Ramos
Summary: The theoretical model on chemical signaling for diagnosis combines signals for marker and inert/interfering metabolites, resulting in asymptotic relationships between signal intensities. Voltammetric features associated with oxidation of urine metabolites and Zn (II) reduction were well-matched with the model, leading to a non-invasive electrochemical detection of bladder cancer. Different diagnostic criteria displayed high sensitivity, specificity, and positive/negative predictive values.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Oncology
Justyna Raczkowska, Agnieszka Bielska, Adam Kretowski, Magdalena Niemira
Summary: Non-small cell lung cancer (NSCLC) is a malignant condition resistant to advanced-stage treatment and the leading cause of cancer death worldwide. Current diagnostic methods cannot detect the disease at an early stage or predict its development. Micro RNAs (miRNAs) show significant differences in NSCLC tissues and can be easily detected in biofluids without invasive collection techniques. Many studies support the importance of miRNAs as a promising biomarker for detecting and predicting NSCLC. This review discusses the role of biofluid miRNAs as a diagnostic tool in NSCLC and highlights future areas of interest.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biophysics
Melanie MacGregor, Hanieh Safizadeh Shirazi, Kit Man Chan, Kola Ostrikov, Kym McNicholas, Alex Jay, Michael Chong, Alexander H. Staudacher, Thomas D. Michl, Aigerim Zhalgasbaikyzy, Michael P. Brown, Moein Navvab Kashani, Adam Di Fiore, Alex Grochowski, Stephen Robb, Simon Belcher, Jordan Li, Jonathan M. Gleadle, Krasimir Vasilev
Summary: The non-invasive diagnostic platform presented in this study combines microfluidic technology with PDD principles and immunocapture technology to detect shed bladder cancer cells in patient urine, achieving highly specific capture rates. In a pilot study, it successfully differentiated bladder cancer patients from non-cancer controls, showing potential for post-treatment surveillance and cancer detection in suspected bladder cancer patients.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Regina Belugina, Evgenii Karpushchenko, Aleksandr Sleptsov, Vladimir Protoshchak, Andrey Legin, Dmitry Kirsanov
Summary: This study explores the feasibility of using a simple electrochemical multisensor system to distinguish between urine samples from patients with confirmed bladder cancer and healthy volunteers. The best model achieved good accuracy and sensitivity with data from individuals of different ages and tumor grades, with even better performance observed in a limited age group. The proposed screening method shows promise for further research due to its simplicity.
Review
Medicine, General & Internal
Ozal Beylerli, Manuel de Jesus Encarnacion Ramirez, Alina Shumadalova, Tatiana Ilyasova, Mikhail Zemlyanskiy, Aferin Beilerli, Nicola Montemurro
Summary: Diagnosing and predicting brain tumors is challenging, and current treatment methods are unsatisfactory. Cell-free miRNA profiling in cerebrospinal fluid (CSF) shows high potential in providing precise and specific evaluations of brain tumors, making it necessary to develop reliable and minimally invasive biomarkers for diagnosis.
Article
Biophysics
Mohammed Zniber, Parastoo Vahdatiyekta, Tan-Phat Huynh
Summary: Electronic tongues have been widely used in quality monitoring and disease diagnosis. However, their application in cancer diagnosis from urine samples is underestimated. This review highlights various analytical techniques and machine learning methods used in e-tongues for urine analysis, emphasizing their ability to distinguish between patients diagnosed with cancer and healthy controls.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Oncology
Camilla De Carlo, Marina Valeri, Devin Nicole Corbitt, Miriam Cieri, Piergiuseppe Colombo
Summary: This review summarizes the recent evidence on prognostic and predictive tissue biomarkers in non-muscle invasive bladder cancer (NMIBC), highlighting the importance of histological features and molecular events in predicting recurrence and progression. The review also emphasizes the potential of immunohistochemical evaluation as a cost-effective tool for clinical practice.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Fernando Lozano, Carles X. X. Raventos, Albert Carrion, Carme Dinares, Javier Hernandez, Enrique Trilla, Juan Morote
Summary: The urine biomarker XBM can predict early high-risk recurrences in non-muscle invasive bladder cancer (NMIBC), but it has low sensitivity and cannot replace cystoscopy and washing cytology.
Article
Immunology
Weizhuo Wang, Xi Zhang, Silin Jiang, Peng Xu, Kang Chen, Kai Li, Fei Wang, Xiang Le, Ke Zhang
Summary: This study aimed to construct a prognostic model based on differential gene expression between muscular and non-muscular invasive bladder cancer (BCa), and to investigate the prognosis of BCa patients. BCa patient data from GEO and TCGA databases were analyzed, and single-cell sequencing and microarray data were used to validate the differential gene expression. The prognostic model showed accuracy and validity, and S100A9 may be a better prognostic marker and therapeutic target for BCa.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Analytical
Li Yan, Congcong Shen, Pengjun Jiang, Yunjin Bai, Runlian Qu, Wu Peng, Zeliang Wei, Ke Huang, Jing Zhou, Rundong Zhang, Piaopiao Chen
Summary: This study proposed a homogeneous enzyme-free and nucleic acid-based catalytic hairpin assembly and cascade recognition reactions strategy for the dual visualization and fluorescence signal detection of bladder cancer marker miRNA-126 in clinical urine. The sensitivity of miRNA analysis was significantly improved by integrating cascade amplification and material cation exchange reaction. The experimental results were consistent with other detection methods and the assay showed high specificity and sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Oncology
Takashi Karashima, Susumu Umemoto, Takeshi Kishida, Kimito Osaka, Masatoshi Nakagawa, Eisaku Yoshida, Toru Yoshimura, Masahiko Sakaguchi, Hiroyuki Nishimoto, Mami Tai, Keiji Inoue, Motoharu Seiki, Naohiko Koshikawa, Taro Shuin
Summary: The study suggests that urine Ln-gamma 2m may be a potentially useful biomarker for non-muscle-invasive bladder cancer, particularly in cases of low-grade cancer.
Article
Biochemistry & Molecular Biology
Magdalena Skalnikova, Katerina Stano Kozubik, Jakub Trizuljak, Zuzana Vrzalova, Lenka Radova, Kamila Reblova, Radka Holbova, Terezia Kurucova, Hana Svozilova, Jiri Stika, Ivona Blahakova, Barbara Dvorackova, Marie Prudkova, Olga Stehlikova, Michal Smida, Leos Kren, Petr Smejkal, Sarka Pospisilova, Michael Doubek
Summary: Bernard-Soulier syndrome (BSS) is a rare inherited disorder characterized by large platelets, low platelet count, and prolonged bleeding time. This study identifies a likely pathogenic variant causing monoallelic BSS and provides insight into the clinical presentation and phenotype of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Martin Krkoska, Jana Nekvindova, Katerina Nevedelova, Veronika Zubanova, Lenka Radova, Jan Vondracek, Jarmila Herudkova, Ondrej Slaby, Igor Kiss, Lucia Bohovicova, Pavel Fabian, Zuzana Tylichova, Zdenek Kala, Petr Kysela, Lenka Ostrizkova, Vladimir Palicka, Alena Hyrslova Vaculova
Summary: The study revealed a potential link between miRNAs and downregulation of drug-metabolizing CYPs in HCC tumors, with miR-29c and miR-653 acting as efficient suppressors of CYP1A2 or AHR expression. This highlights a novel role of miR-653 and miR-29c in regulating CYPs involved in crucial biotransformation processes in liver, often deregulated during liver cancer progression.
PHARMACOLOGICAL REPORTS
(2022)
Article
Oncology
Michal Stanik, Alexander Poprach, Simona Littnerova, Ivo Capak, Marketa Hulova, Natalia Sebova, Radek Lakomy, Jiri Jarkovsky, Jan Dolezel
Summary: Aggressive surgical therapy is feasible for isolated retroperitoneal recurrence in renal cancer patients after radical nephrectomy, with more than half of the patients achieving long-term survival. Prognostic factors of poor outcome include an interval from nephrectomy to recurrence of less than 12 months, tumor grade 3-4, and female sex. Metastasis-directed therapy may prolong the time to initiation of systemic treatment in cases of disease progression.
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2022)
Article
Surgery
Tomas Novotny, Robert Staffa, Josef Tomandl, Tomas Krivka, Ondrej Slaby, Lubos Kubicek, Ernest Biros, Marie Tomandlova, Robert Vlachovsky, Jan Sponiar, Lenka Radova, Anna Konieczna
Summary: This study aimed to evaluate the value of L-lactate as a marker for early detection of postoperative intestinal ischemia. The results showed that measuring serum L-lactate levels at proper timepoints might help in the early diagnosis of postoperative intestinal ischemia after aortic surgery.
INTERNATIONAL JOURNAL OF SURGERY
(2022)
Review
Biochemistry & Molecular Biology
Katerina Honigova, Jiri Navratil, Barbora Peltanova, Hana Holcova Polanska, Martina Raudenska, Michal Masarik
Summary: Metabolic plasticity is an important characteristic of cancer cells in tumorigenesis, allowing them to reshape metabolic pathways to support increased energy demands. The selective pressures in the tumor microenvironment force cancer cells to adapt by reorganizing their metabolism. Understanding metabolic reprogramming is crucial for developing targeted therapies for cancer.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Oncology
Jana Halamkova, Lucia Bohovicova, Lucie Pehalova, Roman Gonec, Teodor Stanek, Tomas Kazda, Lucie Moukova, Dagmar Adamkova Krakorova, Sarka Kozakova, Marek Svoboda, Regina Demlova, Igor Kiss
Summary: The association between hypolipidemic drugs and the occurrence of a second primary malignancy in colorectal cancer survivors is not clear. Subgroup analysis shows a lower incidence of secondary neoplasia in statin users, but colorectal cancer patients using hypolipidemic drugs are more likely to develop gastric and bladder cancer. Patients with early-stage colorectal carcinoma who suffered a second cancer have significantly worse survival outcomes if treated with hypolipidemic drugs. Therefore, screening for second primary malignancies in colorectal cancer survivors treated with hypolipidemic drugs should be done more cautiously, especially for gastric and bladder cancer.
Article
Oncology
Barbora Peltanova, Hana Holcova Polanska, Martina Raudenska, Jan Balvan, Jiri Navratil, Tomas Vicar, Jaromir Gumulec, Barbora Cechova, Martin Krater, Jochen Guck, David Kalfert, Marek Grega, Jan Plzak, Jan Betka, Michal Masarik
Summary: This study delves into the interaction between cancer-associated fibroblasts (CAFs) and head and neck cancer cells, illustrating their impact on the metabolic state, mechanobiology, and aggressiveness of cancer cells. The findings suggest that the growth and progression of tumors require the right collaboration between cancer cells and CAFs, with specific mRNA subtypes playing a crucial role in supporting primary or metastatic cancer cells. Furthermore, the research indicates that the progression of HNSCC may be influenced by the support of CAFs in the tumor microenvironment, and the heterogeneity of this disease may be attributed to the level of cooperation between CAFs and epithelial cancer cells.
Article
Medicine, General & Internal
Milos Holanek, Iveta Selingerova, Pavel Fabian, Oldrich Coufal, Ondrej Zapletal, Katarina Petrakova, Tomas Kazda, Roman Hrstka, Alexandr Poprach, Maria Zvarikova, Ondrej Bilek, Marek Svoboda
Summary: This retrospective study suggests that residual cancer burden after neoadjuvant therapy is associated with worse treatment outcomes in breast cancer patients. The study evaluated the dynamics and impact of biomarkers on long-term outcomes in specific breast cancer subtypes. The proliferation index Ki-67 was significantly altered by neoadjuvant therapy and predicted survival outcomes in different subtypes.
Article
Environmental Sciences
Dita Kadlcikova, Petra Musilova, Hana Hradska, Miluse Vozdova, Marketa Petrovova, Marek Svoboda, Jiri Rubes
Summary: The study investigated occupationally induced chromosomal damage in hospital personnel exposed to antineoplastic drugs and/or low doses of ionizing radiation. Both cytogenetic methods showed an increase in chromatid and chromosomal aberrations, as well as unstable aberrations, indicating recent exposure to genotoxic agents.
ARCHIVES OF ENVIRONMENTAL & OCCUPATIONAL HEALTH
(2023)
Article
Medical Laboratory Technology
Marie Tomandlova, Tomas Novotny, Robert Staffa, Jindra Smutna, Tomas Krivka, Peter Kruzliak, Ondrej Slaby, Lubos Kubicek, Robert Vlachovsky, Lenka Radova, Josef Tomandl
Summary: This study investigates the perioperative kinetics of D-lactate and ischemia-modified albumin (IMA) and their predictive value in early diagnosis of acute intestinal ischemia. The results suggest that serum levels of IMA and D-lactate can serve as influential predictive markers for postoperative intestinal ischemia, especially after 3 hours from complete declamping of vascular reconstruction.
CLINICAL BIOCHEMISTRY
(2023)
Article
Public, Environmental & Occupational Health
Joan Prades, Annalisa Trama, Paolo G. Casali, Jean-Francois Emile, Nathalie Gaspar, Ramunas Janavicius, Rasa Janciauskiene, Sakari Karjalainen, Katerina Kopeckova, Liisa Pylkkanen, Marek Svoboda, Josep M. Borras
Summary: This study aims to explore the processes and principles of embedding reference centers (RCs) for rare cancers in national health systems. The results show heterogeneity in the formalization of RCs' status and their integration in different health systems, with two models (center-based and network-based) envisioned at the national level. Expert clinicians in some countries cooperate in a structured way using network mechanisms and provide a referral system to facilitate access to treatment for patients from non-expert centers.
EUROPEAN JOURNAL OF PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Monika Vlachova, Jana Gregorova, Petra Vychytilova-Faltejskova, Natalia Anna Gablo, Lenka Radova, Lenka Pospisilova, Martina Almasi, Martin Stork, Zdenka Knechtova, Jiri Minarik, Tereza Popkova, Tomas Jelinek, Roman Hajek, Ludek Pour, Lucie Rihova, Sabina Sevcikova
Summary: In this study, antigens and miRNAs involved in the pathogenesis of EMD were identified using flow cytometry and next-generation sequencing. MiR-26a-5p and miR-30e-5p were found to be down-regulated in EMD. Aberrant miRNAs associated with cell cycle regulation, proteolysis, and infection or neurological disorder-related signaling pathways were observed. Additionally, clinicopathological characteristics and CD antigens associated with MM and EMD prognosis were identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Jaroslav Juracek, Marie Madrzyk, Michal Stanik, Michaela Ruckova, Karolina Trachtova, Hana Malcikova, Eva Lzicarova, Dominik A. Barth, Martin Pichler, Ondrej Slaby
Summary: This study identified a miRNA expression pattern associated with disease aggressiveness in prostate cancer patients and established a prognostic score combining miRNA levels and serum PSA level that can distinguish between benign and aggressive prostate cancer.
Article
Hematology
Veronika Navrkalova, Karla Plevova, Lenka Radova, Jakub Porc, Karol Pal, Jitka Malcikova, Sarka Pavlova, Michael Doubek, Anna Panovska, Jana Kotaskova, Sarka Pospisilova
Summary: Large-scale next-generation sequencing studies have revealed the extensive genetic heterogeneity and clonal evolution in chronic lymphocytic leukaemia (CLL). This study investigates the dynamics and impact of subpopulations before therapy initiation and identifies four groups of patients with different clinical behavior based on aberration dynamics. Genetic testing plays a clinically relevant role in long-term patient monitoring and prognosis assessment.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
