4.0 Article

Detection of glycan-binding proteins using glycan-functionalized quantum dots and gold nanoparticles

Journal

JOURNAL OF CARBOHYDRATE CHEMISTRY
Volume 37, Issue 4, Pages 199-209

Publisher

TAYLOR & FRANCIS INC
DOI: 10.1080/07328303.2018.1451875

Keywords

glycan-binding protein; concanavalin A; fluorescence resonance energy transfer; gold nanoparticles; quantum dots

Funding

  1. National Natural Science Foundation of China [31770862, 81661148055, 31470801]
  2. National Key R & D Program of China [2016YFE0205800]
  3. Open Project Program of the State Key Laboratory of Microbial Resources, Institute of Microbiology, CAS [SKLMR-20160604]

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Many pathogens can use their glycan-binding proteins (GBPs) to interact with glycan receptors on host cell surface. Here, a novel strategy is presented for the detection of GBPs based on fluorescence resonance energy transfer (FRET). Using mannosefunctionalized quantum dots and mannose-functionalized gold nanoparticles, a FRET assay system was developed and a mannose-specific GBP concanavalin A could be detected in 5 min with a detection limit of 3 nM. This result indicated the potential for rapid and sensitive biosensing of various kinds of GBPs and pathogens by changing the glycan structure on the surface of QDs and AuNPs. [GRAPHICS] .

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