Article
Biochemistry & Molecular Biology
Aleksandar Dolashki, Olga Antonova, Lyudmila Velkova, Dimitar Kaynarov, Wolfgang Voelter, Pavlina Dolashka
Summary: This study demonstrates the selective cytotoxic effect of molluscan hemocyanins (Hcs) against T24 cancer cell line. The FU beta c-HlH-h shows the strongest anti-proliferative effect, predominantly inducing apoptosis in tumor cells.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Genetics & Heredity
Gilnei Bruno da Silva, Daiane Manica, Alana Patricia da Silva, Greicy Cristine Kosvoski, Marceli Hanauer, Charles Elias Assmann, Julia Leao Batista Simoes, Micheli Mainardi Pillat, Jessica Dotto de Lara, Filomena Marafon, Amanda Gollo Bertollo, Maiqueli E. D. Mingoti, Jullye Gavioli, Gislaine Zilli Reus, Gabriela Goncalves de Oliveira, Zuleide Maria Ignacio, Margarete Dulce Bagatini
Summary: This study investigated the changes in purinergic signaling and immune response in COVID-19 patients. The results showed altered purinergic signaling in both moderate and severe cases, including increased ATP hydrolysis in platelets and PBMCs, as well as significantly elevated ADP and AMP hydrolysis. The study also observed increased expression of CD39 and CD73 in total leukocytes, along with higher extracellular ATP levels in both groups. Additionally, moderate and severe cases exhibited elevated levels of IL-2, IL-6, IL-10, and IL-17. These findings provide valuable insights into the changes in purinergic signaling and immune response in COVID-19, highlighting the therapeutic potential of the purinergic system as a target for the treatment of SARS-CoV-2 disease.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2022)
Review
Biochemistry & Molecular Biology
Lu Wang, Ying-Jie Li, Xu Yang, Bo Yang, Xin Zhang, Jing Zhang, Qi Zhang, Xu-Dong Cheng, Jian-Hong Wang, Neng-Wei Yu
Summary: This review mainly introduces the role and potential mechanisms of purinergic signaling in the treatment of ischemic stroke. By regulating purinergic receptors, purinergic signaling can protect against thrombosis and inhibit inflammation, thereby exerting neuroprotective effects.
PURINERGIC SIGNALLING
(2023)
Article
Cell Biology
Elizabeth H. Zhong, Carola Ledderose, Paola De Andrade Mello, Keiichi Enjyoji, Justin Mark Lunderberg, Wolfgang Junger, Simon C. Robson
Summary: This study demonstrates the synergistic effects of ectoenzymes such as CD39 and CD73 in reducing inflammatory responses, suggesting that these bifunctional enzymes could serve as therapeutic agents in inflammatory diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Endocrinology & Metabolism
Bynvant Sandhu, Maria C. Perez-Matos, Stephanie Tran, Garima Singhal, Ismail Syed, Linda Feldbruegge, Shuji Mitsuhashi, Julie Pelletier, Jinhe Huang, Yusuf Yalcin, Eva Csizmadia, Shilpa Tiwari-Heckler, Keiichi Enjyoji, Jean Sevigny, Eleftheria Maratos-Flier, Simon C. Robson, Z. Gordon Jiang
Summary: This study investigated the impact of NTPDase3 deficiency on diet-induced obesity and metabolism in mice. The results showed that global NTPDase3 deletion led to resistance to obesity and glucose intolerance, with metabolic changes in adipocytes playing a key role rather than NTPDase3 expression in pancreatic beta-cells.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Article
Multidisciplinary Sciences
Johannes Eberhard, Daniela Hirsch, Oliver Schilling, Wilhelm G. Dirks, Feng Guo, Alice Fabarius, Felix Rueckert, Christoph Reissfelder, Peter Hohenberger, Prama Pallavi
Summary: Carcinosarcoma of the urinary bladder is a rare and aggressive subtype of bladder cancer. Limited information is available on its clinical features and treatments due to its rarity. The newly established bladder carcinosarcoma cell line MaS-3 shows chemotherapy sensitivity and resemblance to the original tumor.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Mariana Chaves, Luiz Eduardo Savio, Robson Coutinho-Silva
Summary: Leishmaniasis is a neglected tropical disease that poses a public health problem in developing countries. The interaction between Leishmania parasites, extracellular nucleotides, and purinergic receptors plays a crucial role in determining disease control or dissemination.
BIOMEDICAL JOURNAL
(2022)
Review
Oncology
Xuhui Bao, Liyi Xie
Summary: Radiotherapy not only damages cancer cells directly, but also induces immunogenic cell death (ICD) which activates the host's antitumor immune response in the tumor immune microenvironment (TIME). This review focuses on the role of adenosine triphosphate (ATP) and adenosine (ADO) in regulating antitumor immunity in TIME, particularly after irradiation. It summarizes the mechanisms through which ATP and ADO promote tumor growth and suppress antitumor immunity, as well as promising therapeutic strategies targeting ATP receptors and ectonucleotidases CD39 and CD73. These strategies have the potential to enhance radiotherapy as a combination antitumor treatment.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Cell Biology
Fabricia Dietrich, Angelica Regina Cappellari, Eduardo Cremonese Filippi-Chiela, Patricia Boni de Paula, Julia Brandt de Souza, Stefano Walter Agatti, Roberta Andrejew, Rafael Roesler, Fernanda Bueno Morrone, Ana Maria Oliveira Battastini
Summary: Through clinical data and in vitro experiments, we found that the P2X6 receptor plays a prognostic role in bladder cancer. High expression of P2X6 receptor is associated with increased survival rate in patients. After radiotherapy, P2X6 receptor expression is upregulated in T24 cells but not in RT4 cells.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Oncology
Fuchao Liang, Fei Zhou, Xiaoyuan Qian, Linghui Qin, Jiange Wang, Chen Ding, Yasen Kuniduzi, Xuejun Zhang, Lianming Fan
Summary: This study suggests that radiotherapy improves the survival benefits for patients with small cell carcinoma of the bladder who undergo bladder-sparing surgery. Furthermore, radiotherapy combined with chemotherapy demonstrates a greater survival benefit compared with chemotherapy alone.
FRONTIERS IN ONCOLOGY
(2023)
Article
Physiology
Gabriella L. Robilotto, Olivia J. Yang, Firoj Alom, Richard D. Johnson, Aaron D. Mickle
Summary: Urothelial cells play an important role in both barrier function and sensory function in the bladder. A mouse model using optogenetics was developed to directly stimulate urothelial cells and study their communication with sensory neurons.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Andrology
Qiang Ma, Chen Xu, Xiaomin Han, Xinxin Wang, Wei Zhang, Zhi Liu, Rihan Wu, Fei Wu, Xiaohui Liu, Tengteng Zhang, Yan Su, Kewen Zheng, Yukun Wang
Summary: This study found that HuR promotes the proliferation and migration of T24 cells and inhibits cell apoptosis, possibly through the expression of cyclin D and apoptosis-related protein Bcl-2.
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2022)
Article
Microbiology
Philip T. Lange, Blossom Damania
Summary: EBV is a common pathogen that causes significant lymphoma burden worldwide. In the treatment of EBV-driven lymphoma, targeting the purinergic signaling pathway may be a promising immunotherapeutic target.
Article
Chemistry, Multidisciplinary
Federica Rinaldi, Linda Maurizi, Jacopo Forte, Massimiliano Marazzato, Patrizia Nadia Hanieh, Antonietta Lucia Conte, Maria Grazia Ammendolia, Carlotta Marianecci, Maria Carafa, Catia Longhi
Summary: The study demonstrated that oil-in-water nanoemulsions are an effective approach to improve the bioavailability of Resveratrol and significantly decrease the viability of bladder cancer cells.
Article
Biochemistry & Molecular Biology
Sabrina Gessler, Clara Guthmann, Vera Schuler, Miriam Lilienkamp, Gerd Walz, Toma Antonov Yakulov
Summary: Acute kidney injury is a common complication of severe human diseases, and the mechanisms of repair after injury are still poorly understood. Using a zebrafish model, this study reveals the crucial role of nucleotide-dependent signaling in the cell migration process following tubular injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medical Laboratory Technology
Pamela Rossi Menegotto, Mariela Granero Farias, Fabiane Spagnol, Marina Siebert, Eduardo Cremonese Filippi-Chiela, Ana Paula Alegretti, Diogo Andre Pilger
Summary: This study compared three non-sequencing protocols for detecting major NPM1 mutations and evaluated nuclear morphometric analysis as an alternative method for cuplike blast detection. The results showed that quantitative PCR (qPCR) is the best option to identify NPM1 mutations, with ARMS-PCR being a cheaper alternative. Multiparameter flow cytometry (MFC) can be used as a screening method.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2023)
Article
Chemistry, Medicinal
Afnan Shahin, Seyed-Omar Zaraei, Bilal O. AlKubaisi, Saif Ullah, Hanan S. Anbar, Randa El-Gamal, Varsha Menon, Mohammed S. Abdel-Maksoud, Chang-Hyun Oh, Raafat El-Awady, Nicolly Espindola Gelsleichter, Julie Pelletier, Jean Sevigny, Jamshed Iqbal, Taleb H. Al-Tel, Mohammed El-Gamal
Summary: A series of adamantyl carboxamide derivatives containing sulfonate or sulfonamide moiety were designed and evaluated for their antiproliferative activity against NCI-60 cancer cell lines panel. Compounds 1e and 1i exhibited potent and broad-spectrum inhibition against multiple cancer subtypes while showing safety towards normal cells.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Review
Chemistry, Medicinal
Gustavo Machado das Neves, Luciano Porto Kagami, Ana Maria Oliveira Battastini, Fabricio Figueiro, Vera Lucia Eifler-Lima
Summary: Purinergic signaling is being explored as a potential therapeutic approach for cancer treatment. ATP acts as a chemotactic agent and has in vitro antineoplastic activity, while adenosine functions as an immunosuppressor and is associated with neoangiogenesis, vasculogenesis, and evasion from the immune system. Inhibitors of ecto-5'-NT can prevent tumor progression, lower adenosine concentrations, and impede cancer growth by preventing immune system evasion.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Cell Biology
Liliana I. Sous Naasani, Jean Sevigny, Veronique J. Moulin, Marcia Rosangela Wink
Summary: In this study, the impact of purinergic receptor agonists on wound healing was investigated using self-assembled skin substitutes (SASS). The results showed that treatment with UTP accelerated wound healing, increased proliferation of keratinocytes and fibroblasts, and did not affect skin quality. Furthermore, the presence of ectoenzymes NTPDase1 and NPP1 in the wounded skin suggests their involvement in the wound healing process. Although these results are preliminary, they highlight the importance of understanding the role of extracellular nucleotides in skin healing and the need for further experiments to determine the underlying mechanisms.
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2023)
Article
Chemistry, Medicinal
Saquib Jalil, Saif Ullah, Seyed-Omar Zaraei, Rawan M. Sbenati, Afnan I. Shahin, Bilal O. AlKubaisi, Julie Pelletier, Jean Sevigny, Taleb H. Al-Tel, Jamshed Iqbal, Mohammed I. El-Gamal
Summary: Inhibition of carbonic anhydrases is being explored as a potential treatment option for several pathological conditions. This study synthesized a series of 4-benzamidophenyl sulfamate derivatives and tested their inhibitory activity against two isoforms of carbonic anhydrase. The synthesized compounds showed potent inhibition of both isoforms at micromolar concentrations, with compound 1n and 1f being the most potent inhibitors for CA II and CA IX, respectively. Molecular modeling studies provided insights into the interactions between these compounds and the active sites of the enzymes.
MEDICINAL CHEMISTRY RESEARCH
(2023)
Article
Cell Biology
Xiaofeng Huang, Wei Gu, Jiaoyue Zhang, Ying Lan, Jonathan L. Colarusso, Sanlan Li, Christoph Pertl, Jiaqi Lu, Hyunkee Kim, Jian Zhu, David T. Breault, Jean Sevigny, Qiao Zhou
Summary: Huang et al. developed a method to generate pancreatic islet-like organoids from human gastric stem cells, which can restore glucose homeostasis in diabetic mice. This study provides a promising approach for cell therapy in diabetes by differentiating gastric stem cells into insulin-secreting cells that resemble beta-cells. The transplanted organoids successfully restored glucose balance for over 100 days in diabetic mice.
NATURE CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Juliete Nathali Scholl, Augusto Ferreira Weber, Camila Kehl Dias, Vinicius Pierdona Lima, Lucas Kich Grun, Diego Zambonin, Eduardo Anzolin, Wanderson Willian Dos Santos Dias, Willian Pegoraro Kus, Florencia Barbe-Tuana, Ana Maria Oliveira Battastini, Paulo Valdeci Worm, Fabricio Figueiro
Summary: Malignant gliomas are aggressive and invasive tumors with a poor prognosis. The interactions between glioma cells and other components of the tumor microenvironment pose a challenge for effective treatment strategies. This study explores the purinergic signaling and ectoenzymes involved in the metabolism of extracellular nucleotides in the glioma microenvironment, with a focus on lower-grade gliomas and the differences between IDH-Mut and IDH-WT gliomas. The findings suggest that the increased ectoenzymes in the tumor microenvironment contribute to immunosuppression.
PURINERGIC SIGNALLING
(2023)
Article
Cell & Tissue Engineering
Jose Bernardo Noronha-Matos, Rui Pinto-Cardoso, Catarina Bessa-Andres, Maria Teresa Magalhaes-Cardoso, Fatima Ferreirinha, Maria Adelina Costa, Jose Marinhas, Rolando Freitas, Rui Lemos, Adelio Vilaca, Antonio Oliveira, Julie Pelletier, Jean Sevigny, Paulo Correia-de-Sa
Summary: This study found that post-menopausal women have impaired osteogenic potential in their bone marrow-derived mesenchymal stromal cells (BM-MSCs) due to overexpression of the nucleotide metabolizing enzyme NTPDase3. Inhibiting NTPDase3 or silencing its gene expression can restore the osteogenic potential of these cells.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Immunology
Dominique S. Rubenich, Priscila O. de Souza, Natalia Omizzollo, Mariana R. Aubin, Paulo J. Basso, Luisa M. Silva, Eloisa M. da Silva, Fernanda C. Teixeira, Gabriela F. S. Gentil, Jordana L. Domagalski, Maico T. Cunha, Kerolainy A. Gadelha, Leonardo F. Diel, Nicolly E. Gelsleichter, Aline S. Rubenich, Gabriela S. Lenz, Aline M. de Abreu, Giselle M. Kroeff, Ana H. Paz, Fernanda Visioli, Marcelo L. Lamers, Marcia R. Wink, Paulo V. Worm, Anelise B. Araujo, Jean Sevigny, Niels O. S. Camara, Nils Ludwig, Elizandra Braganhol
Summary: This study demonstrates that neutrophils are reprogrammed by the tumor to ultimately promote the progression of glioblastoma (GB). The communication between GB and neutrophils directly promotes an immunosuppressive tumor microenvironment (TME). These findings are crucial for understanding tumor progression and the role of immune cells in this process.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Vittoria Lopez, H. J. Maximilian Schuh, Salahuddin Mirza, Victoria J. Vaassen, Michael S. Schmidt, Katharina Sylvester, Riham M. Idris, Christian Renn, Laura Schaekel, Julie Pelletier, Jean Sevigny, Annamaria Naggi, Bjoern Scheffler, Sang-Yong Lee, Gerd Bendas, Christa E. Mueller
Summary: The study discovered that heparin and its derivatives are potent and selective allosteric inhibitors of the poorly investigated ectonucleotidase NPP1. The inhibition of NPP1 by heparins may contribute to their anti-cancer effects and could lead to improved therapeutics for cancer immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Zahid Hussain Zaigham, Saif Ullah, Julie Pelletier, Jean Sevigny, Jamshed Iqbal, Abbas Hassan
Summary: The aim of this research is to synthesize sulfamoyl-benzamides as selective inhibitors of h-NTPDases. Various sulfonamides and carboxamides with biologically relevant substituents were synthesized and screened for their inhibitory activity against h-NTPDases isoforms. The most potent inhibitors were identified for each isoform, and molecular docking studies showed significant interactions with the target proteins.
Article
Oncology
Helouise Richardt Medeiros, Jose Antonio Fagundes Assumpcao, Dirson Joao Stein, Eduardo Cremonese Filippi-Chiela, Felipe Fregni, Wolnei Caumo, Paulo Roberto Stefani Sanches, Iraci L. S. Torres
Summary: The viability of SH-SY5Y neuroblastoma cells was reduced after 24 h and 6 days of exposure to SMS (p<0.05), while there were no differences for the other time points (p>0.05). However, this effect was not associated with cell death or cell cycle arrest (p>0.05). In contrast, the viability of HMV-II melanoma cells, used as a control, was not affected. Additionally, stimulated SH-SY5Y cells showed a decrease in mitochondrial mass at both exposure times and a reduction in autophagy and cell proliferation after 6 days (p<0.05).
ANTICANCER RESEARCH
(2023)
Meeting Abstract
Endocrinology & Metabolism
Ilian Radichev, Jean Sevigny, Julie Pelletier, Clayton Mathews, Kurt Griffin, Alexei Savinov
HORMONE RESEARCH IN PAEDIATRICS
(2023)
Article
Biochemistry & Molecular Biology
Liliana Ivet Sous Naasani, Jessica Gonsalves Azevedoa, Jean Sevigny, Tiago Franco de Oliveira, Silvya Stuchi Maria-Engler, Marcia Rosangela Wink
Summary: This study reveals the expression and function of ectonucleotidases in human epidermal melanocytes, suggesting their involvement in regulating nucleotide levels in the skin microenvironment.
BIOCHEMISTRY AND CELL BIOLOGY
(2023)
Article
Chemistry, Medicinal
Carla Fernanda Furtado Gardani, Eduardo Luiz Pedrazza, Victoria Santos Paz, Gabriele Goulart Zanirati, Jaderson Costa da Costa, Roberta Andrejew, Henning Ulrich, Juliete Nathali Scholl, Fabricio Figueiro, Liliana Rockenbach, Fernanda Bueno Morrone
Summary: This study investigated the expression of CD39 and CD73 enzymes in prostate cancer and their potential as therapeutic targets. The results showed higher levels of CD39 expression compared to CD73 in tissue samples and a correlation with Gleason score. In blood samples, CD39 expression in extracellular vesicles was significantly increased and positively correlated with ADP hydrolysis and Gleason score.