Journal
JOURNAL OF BIOTECHNOLOGY
Volume 270, Issue -, Pages 21-29Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2018.01.018
Keywords
Cellulomonas fimi; Secretome; Lignocellulose bioconversion; Carbohydrate active enzymes; In silico 2DE; Optimal secretion medium
Categories
Funding
- SPRITE project - PAR-FSC Asse 1.3 Regime di aiuto per la qualificazione e il rafforzamento del sistema produttivo piemontese bis
Ask authors/readers for more resources
Lignocellulose degradation is a challenging step for value added products and biofuels production. Cellulomonas fimi secretes complex mixtures of carbohydrate active enzymes (CAZymes) which synergistically degrade cellulose and hemicelluloses. Their characterization may provide new insights for enzymatic cocktails implementation. Bioinformatic analysis highlighted 1127 secreted proteins, constituting the in silico secretome, graphically represented in a 2DE map. According to Blast2GO functional annotation, many of these are involved in carbohydrates metabolism. In vivo secretomes were obtained, growing C. fimi on glucose, CMC or wheat straw for 24 h. Zymography revealed degradative activity on carbohydrates and proteomic analysis identified some CAZymes, only in secretomes obtained with CMC and wheat straw. An interaction between cellobiohydrolases is proposed as a strategy adopted by soluble multimodular cellulases. Such approach can be crucial for a better characterization and industrial exploitation of the synergism among C. fimi enzymes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available