Article
Biotechnology & Applied Microbiology
Jun Liu, Jingyan Wu, Xinru Cai, Song Zhang, Ying Liang, Qinlu Lin
Summary: This study found that cofactor metabolic engineering strategies for M. purpureus can significantly increase pigment production, especially yellow pigment, while reducing citrinin production.
Article
Biotechnology & Applied Microbiology
Hai-qing Liu, Zhen-feng Huang, Shan-zhong Yang, Xiao-fei Tian, Zhen-qiang Wu
Summary: The study found that La3+ treatment significantly increased the yield of Monascus pigments and biomass, while reducing citrinin production. La3+ treatment not only improved ROS levels and the activities of CAT and SOD, but also enhanced the permeability and fluidity of the cell membrane.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Energy & Fuels
Nazli Pinar Arslan, Aysenur Yazici, Senba Komesli, Nevzat Esim, Serkan Ortucu
Summary: The study utilized loquat kernel powder as a fermentation substrate to produce pigments from Monascus purpureus, investigating the effect of four mineral salts on pigment production. Results showed that the addition of different salts could regulate pigment production, with a combination of three salts leading to increased enzyme and red pigment production. Additionally, optimized medium led to higher concentrations of pigments and enzyme activities in both flask and fermenter cultures.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Microbiology
Yingying Huang, Chenglong Yang, Istvan Molnar, Shen Chen
Summary: In this study, a comparative transcriptomic analysis was performed on high and low citrinin-producing Monascus purpureus strains using RNA-Seq and qRT-PCR. A total of 2518 differentially expressed genes were identified, with 1141 downregulated and 1377 upregulated genes in the low citrinin producer strain. Many upregulated genes were associated with energy metabolism and carbohydrate metabolism, potentially providing more biosynthetic precursors for Monascus pigments synthesis. Additionally, several potentially interesting genes encoding transcription factors were also identified. This study provides valuable insights into metabolic adaptations and safer Monascus pigments production.
Article
Food Science & Technology
Pengfei Gong, Ruoyu Shi, Yutong Liu, Qiaoqiao Luo, Chengtao Wang, Wei Chen
Summary: Red yeast rice (RYR), also known as Monascus purpureus, has a long history of medicinal and culinary use in Asia. This review provides an updated overview of the latest advancements in Monascus pigment (MP) research, including insights into biosynthetic pathways, optimization methods for fermentation, extraction, and stabilization, as well as the health benefits and diverse applications of MPs in the food industry. The review also discusses current limitations and proposes feasible solutions for MP product development and applications to meet the growing global demand.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Jie Qiao, Huawei Zeng, Wenjing Ye, Ran Qiu, Xin Zeng, Bingyue Xin, Tingyu Xie
Summary: In this study, the addition of sucrose esters significantly promoted the production of yellow pigment by Monascus purpureus during submerged fermentation. Integrating the addition of sucrose esters with immobilised fermentation using corn cob as the carrier resulted in a higher yellow pigment yield. Transcriptome analysis revealed that integrative fermentation effectively regulated pyruvate metabolism, improved membrane properties, and increased transport capacity of pigment across the membrane.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Menglin Ju, Jialan Zhang, Tingting Mai, Li Li, Tong Gu, Yingbao Liu, Mengxiang Gao
Summary: This study investigated the interaction effect of Monascus purpureus and Rhodotorula mucilaginosa on growth and metabolism. Co-culturing R. mucilaginosa with different amounts of M. purpureus spores or fermentation filtrate showed significant promotion of Monascus pigments yield but inhibited the growth of M. purpureus. Co-culturing R. mucilaginosa with 1.25 x 104 spores/mL or 50% (v/v) M. purpureus fermentation filtrate significantly promoted the growth and carotenoids production of R. mucilaginosa, resulting in 45.1% and 29.3% increase in carotenoids yield, respectively. The stimulative effect of M. purpureus fermentation filtrate on carotenoids production of R. mucilaginosa might be due to synergistic metabolites produced by M. purpureus. In conclusion, co-culturing R. mucilaginosa with appropriate amounts of M. purpureus spores or fermentation filtrate can increase carotenoids yield, making it an effective method for increasing the yield of carotenoids and Monascus pigments.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Dilara Mehri, N. Altinay Perendeci, Yekta Goksungur
Summary: Various biotechnological approaches have been used to convert food waste into value-added bioproducts through fermentation processes. This study focused on investigating the effect of different fermentation parameters in simultaneous hydrolysis and fermentation of whey for pigment production with Monascus purpureus. Optimal conditions of SHF were 2% (v/v) inoculation ratio, 75 g/L of lactose as carbon source, 25 g/L of MSG as nitrogen source, and fermentation medium pH of 7.0, leading to maximum red pigment production.
FERMENTATION-BASEL
(2021)
Article
Microbiology
Sheng Yin, Dongmei Yang, Yiying Zhu, Baozhu Huang
Summary: The study reveals that amino acid metabolism can regulate the biosynthesis of Monascus pigments (MPs), and methionine and S-adenosylmethionine (SAM) play different roles in the regulation of MPs biosynthesis in Monascus purpureus RP2. The expression of SAM synthetase leads to an increased expression of transcriptional regulatory proteins of the MPs biosynthesis gene cluster, while the addition of SAM results in increased expression of MPs biosynthesis activators and the global regulator LaeA. These findings provide a new perspective for understanding the regulation of MPs biosynthesis in M. purpureus.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Hao Zhang, Huanhuan Liu, Lin Shu, Huimin Xu, Ying Cheng, Zhitao Mao, Bin Liu, Xiaoping Liao, Di Huang
Summary: Monascus azaphilones pigments (MonAzPs) produced by microbial fermentation are widely used as food chemicals for coloring and providing beneficial biological attributes. This study used a fermentation perturbation strategy to investigate the metabolic changes induced by the addition of different amino acids. The results identified fumarate and malate as the hub metabolites involved in the tricarboxylic acid cycle, which played a crucial role in the synthesis of MonAzPs. Exogenous addition of fumarate or malate significantly increased red pigment production while reducing orange/yellow pigment production. The study also highlighted the importance of the tricarboxylic acid cycle in MonAzPs synthesis through measurements of intracellular ATP, NAD(P)H levels, and expression of oxidoreductase-coding genes. Overall, metabolomics is a powerful approach for identifying key metabolic regulatory factors and advancing engineering strategies for the fermented food chemistry industries.
Article
Food Science & Technology
Liuming Xie, Jianhua Xie, XianXiang Chen, Xin Tao, Jiayan Xie, Xiaoyi Shi, Zhibing Huang
Summary: This study optimized the fermentation conditions of Monascus purpureus and performed comparative transcriptomic analysis to understand the mechanisms and effects of fermentation on exopolysaccharides (EPS) synthesis. The study identified key enzymes and enriched carbohydrate metabolism subcategories involved in EPS synthesis. The findings provide a theoretical basis for enhancing EPS content and suggest important targets for future molecular modifications and gene knockdown studies.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Microbiology
Sheng Yin, Yiying Zhu, Bin Zhang, Baozhu Huang, Ru Jia
Summary: This study investigated the effects of 20 free amino acids on Monascus pigments (MPs) biosynthesis in Monascus purpureus RP2. Six amino acids were identified as valuable ones due to their significant effects on production yield and metabolic curves of MPs. Dose-dependent and synergistic effects of these amino acids on MPs biosynthesis were observed. Histidine and methionine were found to have dominant and universal influences on MPs biosynthesis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mehak Kaur, Mayurika Goel, Rahul Chandra Mishra, Vaibhavi Lahane, Akhilesh K. Yadav, Colin J. Barrow
Summary: The acceptability and appeal of food are greatly influenced by color. Harmful effects of synthetic colorants are well known, and current research aims to develop natural, synthetic chemical-free alternatives derived from fungal sources for various applications. This study focused on identifying endophytic marine fungi as natural bio-sources of red pigment, with specific emphasis on the fungus Monascus purpureus CPEF02. The researchers successfully identified the chemical components of the pigmented extracts and explored optimal culture conditions for maximum pigment production. The methanolic fungal colorant extract showed significant antibacterial, antifungal, and antioxidant activities, indicating the potential industrial use of the M. purpureus pigment as a natural red colorant.
FERMENTATION-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Xiaoju Chen, Ruiyu Gui, Nan Li, Yuqing Wu, Ju Chen, Xuefeng Wu, Zhen Qin, Shang-Tian Yang, Xingjiang Li
Summary: This study developed a method using Monascus purpureus for submerged fermentation to convert potato pomace into a high value-added product containing soluble dietary fibers and Monascus pigments. The process yielded red pigments with high thermal stability and also extracted soluble dietary fibers with porous microstructure and antioxidant activities from the potato pomace. This provides an economical and environmentally friendly route for utilizing agro-industrial waste byproduct.
PROCESS BIOCHEMISTRY
(2021)
Article
Food Science & Technology
Qi Mei, Zhaona Xu, Quanye Wu, Likang Qin, Haiying Zeng, Yi Zhu
Summary: This study used LC-MS to investigate the differential metabolites of coix seed after fermentation by Monascus purpureus. The results showed that fermentation significantly increased the contents of amino acids, peptides, and steroids in coix seed metabolites. The findings suggest that Monascus purpureus fermentation can successfully transform coix seed, improving its functional activities.
Article
Environmental Sciences
Senba Komesli, Sumeyya Akbulut, Nazli Pinar Arslan, Ahmet Adiguzel, Mesut Taskin
Summary: This study successfully utilized locally isolated cold-adapted bacteria under non-sterile culture conditions to produce lipase and hydrolyze waste frying oils. The most favorable conditions were found to be low temperature, restricted nutrient availability and high inoculum volume, which effectively prevented microbial contaminants.
ENVIRONMENTAL TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yaprak Ilayda Karadayi, Gurkan Aykutoglu, Nazli Pinar Arslan, Mustafa Ozkan Baltaci, Ahmet Adiguzel, Mesut Taskin
Summary: This study revealed the EPS production potential of A. gonensis and the anticancer activity of the produced EPS. It was shown for the first time that the main monomer of bacterial heteropolysaccharides would be xylose.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Berna Genc, Mesut Taskin, Ahmet Adiguzel
Summary: The study investigated the biological, rheological, and chemical properties of EPS purified from the thermophilic bacterium Anoxybacillus pushchinoensis G11. The EPS showed antibiofilm and antitumor activities, exhibited a heteropolysaccharide composition, and had a molecular weight of 75.5 kDa.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hasan Turkez, Mehmet Enes Arslan, Ahmet Yilmaz, Funda Doru, Ozge Caglar, Elif Arslan, Abdulgani Tatar, Ahmet Hacimuftuoglu, A. M. Abd El-Aty, Adil Mardinoglu
Summary: Propolis is a resinous compound extract transformed by honey bees that exhibits beneficial biological activities including antifungal, antibacterial, antiviral, antioxidant, antimutagenic, and antitumor effects. Recent studies have shown potential neuroprotective effects of propolis, with changes in gene expression profiles indicating involvement of specific genes. However, further research is needed to fully understand the molecular genetic mechanisms and toxicogenomics of propolis on neuronal cells. It can be concluded that propolis supplementation may offer advantages in the prevention and treatment of neurodegenerative disorders.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Engineering, Environmental
Fatih Kayar, Meryem Doymus, Hakan Ozkan, Nazli Pinar Arslan, Ahmet Adiguzel, Mesut Taskin
Summary: In this study, chitinous material was produced from locally isolated Penicillium crustosum using molasses as substrate. Chitosan was prepared from the chitinous material and its antibacterial activity was investigated. The chitosan showed higher antibacterial efficiency compared to commercial chitosan, making it potentially useful for biomedical applications. This study provides important insights into the production and antibacterial properties of chitosan.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Toxicology
Veysel Parlak, Bunyamin Ozgeris, Arzu Ucar, Asli Cilingir Yeltekin, Fatma Betul Ozgeris, Ozge Caglar, Gonca Alak, Hasan Turkez, Muhammed Atamanalp
Summary: This study evaluated the hematological, oxidative, and genotoxic damage potentials of 3-Benzoylpyridine (3BP) on rainbow trout. The results showed that 3BP caused hematological and oxidative alterations as well as genotoxic damage in rainbow trout.
TOXICOLOGY MECHANISMS AND METHODS
(2022)
Article
Biochemistry & Molecular Biology
Ozge Caglar Yildirim, Mehmet Enes Arslan, Sena Oner, Ivana Cacciatore, Antonio Di Stefano, Adil Mardinoglu, Hasan Turkez
Summary: This study developed a boron-based drug delivery system for the treatment of Alzheimer's disease by incorporating the folic acid functional group into hexagonal boron nitride nanoparticles. The system showed promising neuroprotective properties against AD in experimental models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Muhammed Atamanalp, Hasan Turkez, Asli cilingir Yeltekin, Fatma Betuel Ozgeris, Arzu Ucar, Ozge caglar, Veysel Parlak, Sena Oner, Gonca Alak
Summary: The compound acrylamide (AA) is widely used in industries such as paper, dyes, and plastics, but its water solubility suggests it could pose environmental problems. AA has been found to be neurotoxic, immunotoxic, reproductive toxic, and carcinogenic in animals. This study investigated the potential of boron compounds, such as borax (BX), to ameliorate AA-induced toxicities in rainbow trout. The results showed that AA exposure led to oxidative stress and DNA damage in both blood and liver tissues of the fish. However, co-exposure of BX with AA also elicited oxidative stress and cell death. Overall, BX was suggested as a useful and effective natural agent for preventing and treating AA toxicity in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Arzu Ucar, Mehmet Enes Arslan, Asli Cilingir Yeltekin, Fatma Betul Ozgeris, Ozge Caglar Yildirim, Veysel Parlak, Gonca Alak, Hasan Turkez, Muhammed Atamanalp
Summary: The unique features of nanoparticles have led to new applications in various fields. However, the release of nanoparticles into the environment raises concerns. This study evaluated the toxicity of magnetite nanoparticles on aquatic organisms and found that treatment with boron mineral can alleviate the harmful effects.
DRUG AND CHEMICAL TOXICOLOGY
(2023)
Article
Engineering, Environmental
Fatma Betul Ozgeris, Asli Cilingir Yeltekin, Arzu Ucar, Ozge Caglar, Veysel Parlak, Mehmet Enes Arslan, Hasan Turkez, Muhammed Atamanalp, Gonca Alak
Summary: Fe2+ in ferrocene enhances oxidation reactions. Increased iron (Fe) levels in freshwater ecosystems are regarded as an important environmental issue. Factors contributing to increased Fe loading in freshwater environments include changes in land use, mining, industrial activity, and elevated acid deposition. Ferrocene is widely used in organometallic chemistry industry. In this study, the toxicity potential of acute ferrocene exposure and the protective potential of borax supplementation were investigated in rainbow trout. The results showed that ferrocene caused different reactions in blood indexes, and borax application had a healing effect. Ferrocene inhibited antioxidant enzyme activities and increased oxidative stress levels in both blood and liver tissues. The findings suggested that borax may exhibit ameliorative potential against ferrocene-induced toxicity by regulating the ROS/TNF-alpha/Nrf-2 pathway.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2023)
Article
Chemistry, Physical
Mehmet Enes Arslan, Arzu Tatar, Ozge Caglar Yildirim, Irfan Oguz Sahin, Ozlem Ozdemir, Erdal Sonmez, Ahmet Hacimuftuoglu, Metin Acikyildiz, Fatime Geyikoglu, Adil Mardinoglu, Hasan Turkez
Summary: Nanobiotechnology has a significant impact on various industries, and the toxicity potentials of specific nanosized molecules, such as boron-containing nanoparticles (NPs), need to be investigated. In this study, Co2B NPs were characterized and their effects on human pulmonary alveolar epithelial cells (HPAEpiC) were assessed. The results showed that Co2B NPs had an impact on cell viability and gene expression in several pathways. Overall, Co2B NPs could be considered as a safe and effective nanomolecule for industrial applications, especially in the medical field.
Article
Biotechnology & Applied Microbiology
Mehmet Akif Omeroglu, Seyda Albayrak, Nazli Pinar Arslan, Hakan Ozkan, Ahmet Adiguzel, Mesut Taskin
Summary: The present study tested the usability of low-cost sheep wool peptone (SWP) as an organic nitrogen source in the production of six industrially important enzymes. SWP was found to be superior to commercial tryptone peptone (TP) in the production of four enzymes. This study is the first to investigate the usage of sheep wool protein hydrolysate (SWP) as an organic nitrogen source or a peptone in the fermentative production of microbial enzymes.
Article
Pharmacology & Pharmacy
Hasan Turkez, Ozge Caglar Yildirim, Sena Oner, Abdurrahim Kadi, Abdulkadir Mete, Mehmet Enes Arslan, Irfan Oguz Sahin, Omer Erkan Yapca, Adil Mardinoglu
Summary: Complications of chronic non-healing wounds have led to the development of nanotechnology-based therapies using ALA conjugated hBN and B4C nanoparticles to accelerate wound healing and prevent infections. Through experimental analysis, it was found that ALA-conjugated hBN nanoparticles exhibited the highest wound healing potential and antimicrobial activity compared to ALA-B4C. Furthermore, the conjugation of ALA enhanced the wound healing and antimicrobial properties of hBN and B4C nanoparticles.
Article
Materials Science, Biomaterials
Murat Aydemir, Gulcin Haykir, Harun Selvitopi, Ozge Caglar Yildirim, Mehmet Enes Arslan, Bahattin Abay, Figen Turksoy
Summary: Two novel anthracene derivatives were synthesized and their photo-physical and biological properties were investigated. The introduction of cyano (-CN) substitution was found to alter the charge distribution and energy levels. The derivatives exhibited intramolecular charge transfer properties and the photo-physical properties were strongly influenced by the cyano substitution. The compounds showed potential for cellular imaging and had no significant cytotoxicity.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Biology
Ayse Varol, Seyda Albayrak, Hakan Ozkan, Yeliz Demir, Mesut Taskin, Ahmet Adiguzel
Summary: This study aimed to investigate the fibrinolytic enzyme-producing potentials of locally isolated soil bacteria and to purify and characterize the fibrinolytic enzyme of the most potent bacterial isolate. Among 40 isolates, the isolate V4 showed the highest potential. Based on 16S rRNA sequence analysis, the isolate V4 was identified as Bacillus atrophaeus. The optimal parameters for fibrinolytic enzyme production from B. atrophaeus were determined.
