Journal
JOURNAL OF BIOSCIENCES
Volume 43, Issue 3, Pages 555-567Publisher
INDIAN ACAD SCIENCES
DOI: 10.1007/s12038-018-9763-4
Keywords
Fluorescence; fluorescence anisotropy; fluorescence lifetime; fluorescence quenching; Maximum Entropy Method; sample heterogeneity; time-resolved fluorescence
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This article describes the basic principles of steady-state and time-resolved fluorescence. The formal equivalence of the two methodologies is described first, followed by the extra advantages of time-resolved methods in revealing population heterogeneity in complex systems encountered in biology. Several examples from the author's work are described in support of the above contention. Finally, several misinterpretations and pitfalls in the interpretation of fluorescence data and their remedy are described.
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