Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 293, Issue 36, Pages 14089-14099Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA118.003809
Keywords
natural product; carbohydrate chemistry; carbohydrate structure; chemical modification; drug discovery; oligosaccharide; thrombosis
Categories
Funding
- National Natural Science Foundation of China [81673330, 81773737]
- Yunnan Provincial Science and Technology Department [2016FA050, 2010CI116]
- Kunming Institute of Botany [KIB2017011]
- Chinese Academy of Sciences Youth Innovation Promotion Association [2017435]
Ask authors/readers for more resources
Fucosylated glycosaminoglycan (FG), a structurally complex glycosaminoglycan found up to now exclusively in sea cucumbers, has distinct anticoagulant properties, notably a strong inhibitory activity of intrinsic factor Xase complex (FXase). Knowledge of the FG structures could facilitate the development of a clinically effective intrinsic FXase inhibitor for anticoagulant drugs. Here, a new fucosylated glycosaminoglycan was obtained from the widely traded sea cucumber Bohadschia argus. The precise structure was deduced as {4)-[l-Fuc3S4S--(13)-]-d-GlcA--(13)-d-GalNAc4S6S--(1} through analysis of its chemical properties and homogeneous oligosaccharides purified from its -eliminative depolymerized products. The B. argus FG with mostly 3,4-di-O-sulfated fucoses expands our knowledge on FG structural types. This -elimination process, producing oligosaccharides with well-defined structures, is a powerful tool for analyzing the structure of complex FGs. Among these oligosaccharides, an octasaccharide displayed potent FXase inhibitory activity. Compared with oligosaccharides with various degrees of polymerization (3n and 3n - 1), our analyses reveal that the purified octasaccharide is the minimum structural unit responsible for the potent selective FXase inhibition, because the d-talitol in the nonsaccharide is unnecessary. The octasaccharide with 2,4-di-O-sulfated fucoses is more potent than that of one with 3,4-di-O-sulfated fucoses. Thus, sulfation patterns can play an important role in the inhibition of intrinsic factor Xase complex.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available