Article
Biochemistry & Molecular Biology
Paras Sehgal, Samatha Mathew, Ambily Sivadas, Arjun Ray, Jyoti Tanwar, Sushma Vishwakarma, Gyan Ranjan, K. Shamsudheen, Rahul C. Bhoyar, Abhishek Pateria, Elvin Leonard, Mukesh Lalwani, Archana Vats, Rajeev R. Pappuru, Mudit Tyagi, Saumya Jakati, Shantanu Sengupta, B. K. Binukumar, Subhabrata Chakrabarti, Inderjeet Kaur, Rajender K. Motiani, Vinod Scaria, Sridhar Sivasubbu
Summary: The study identified a novel vascular endothelial-associated lncRNA named VEAL2, which regulates endothelial permeability and function through interaction with PRKCB2, and is implicated in diabetic retinopathy.
Review
Immunology
Zhilei Chen, Weici Zhang, Carlo Selmi, William M. Ridgway, Patrick S. C. Leung, Fengchun Zhang, M. Eric Gershwin
Summary: MARCKS and MARCKSL1 are membrane-associated proteins involved in various cellular processes. MARCKS plays a critical role in the sequestration and release of phosphatidylinositol-4,5-diphosphate (PIP2), and is associated with immune function, cancer, psychiatric disorders, and autoimmune diseases.
AUTOIMMUNITY REVIEWS
(2021)
Review
Chemistry, Medicinal
Vikas Yadav, Amarish Kumar Sharma, Gaurav Parashar, Nidarshana Chaturvedi Parashar, Seema Ramniwas, Manoj Kumar Jena, Hardeep Singh Tuli, Kiran Yadav
Summary: This review article provides an overview of the patent landscape and recent developments in peptides targeting MARCKS for therapeutic purposes, especially in the treatment of respiratory diseases. The increasing research studies and patent activities related to MARCKS suggest a potential therapeutic intervention using MARCKS-targeting peptides. The compilation of this review article aims to draw the interest of researchers worldwide to further develop MARCKS-targeting peptides for human diseases.
EXPERT OPINION ON THERAPEUTIC PATENTS
(2023)
Article
Oncology
Ziwei Li, Xiangqi Meng, Pengfei Wu, Caijun Zha, Bo Han, Lulu Li, Nan Sun, Tengfei Qi, Jie Qin, Yangong Zhang, Kaifu Tian, Shupeng Li, Changxiao Yang, Lejia Ren, Jianguang Ming, Pandeng Wang, Yifei Song, Chuanlu Jiang, Jinquan Cai
Summary: This study found that lnc-TALC could be transmitted to tumor-associated macrophages (TAM) via exosomes, promoting M2 polarization and increasing resistance of brain tumors to TMZ chemotherapy. The results suggest that the interaction mediated by lnc-TALC between GBM cells and microglia can reduce the efficacy of chemotherapy, indicating potential combination therapy strategies to overcome drug resistance.
CANCER IMMUNOLOGY RESEARCH
(2021)
Editorial Material
Hematology
Jan A. Burger
Summary: The study reveals that MARCKS protein is differentially expressed in patients with chronic lymphocytic leukemia based on the mutation status of IGHV, and its expression and phosphorylation are linked to CLL cell migration through key signaling pathways. The findings were also confirmed in samples from patients treated with the BTK inhibitor acalabrutinib.
Article
Biochemistry & Molecular Biology
Youming Zhu, Leu Jin, Ronghua Shi, Jinming Li, Yan Wang, Li Zhang, Chao-Zhao Liang, Vinod K. Narayana, David P. De Souza, Rick F. Thorne, Li Rong Zhang, Xu Dong Zhang, Mian Wu
Summary: This study reveals a novel long noncoding RNA (lncRNA) called gLINC that acts as a backbone for the formation of a metabolon involving glycolytic enzymes and lactate dehydrogenase A. The gLINC metabolon enhances glycolytic flux, ATP production, and cell survival under serine deprivation. Overexpression of gLINC in cancer cells promotes xenograft growth in mice on a serine-deprived diet, suggesting its role in cancer cell adaptation.
Article
Cell Biology
Yuanxiu Gan, Jie Long, Yan Zeng, Yan Zhang, Yang Tao
Summary: This study screened differentially expressed lncRNAs and constructed an lncRNA-miRNA-mRNA network based on transcriptome data. It identified the IL-17RA-1/miR-7847-3p/PRKCG coexpression network and verified its effect in an LPS-induced sepsis model. The findings suggest that this competing endogenous RNA (ceRNA) network may be a potential approach to predict and combat sepsis.
MEDIATORS OF INFLAMMATION
(2022)
Article
Biochemical Research Methods
Haixiang Zhang, Yinan Zheng, Lifang Hou, Cheng Zheng, Lei Liu
Summary: In this study, a novel method is introduced to identify potential mediators in high-dimensional Cox regression, with successful control of false discovery rate through data dimension reduction and multiple-testing procedure. Application to DNA methylation markers in lung cancer patients reveals two potential mediating epigenetic markers.
Article
Plant Sciences
Mana Morishita, Kohei Hada, Masaki Kita, Toshio Nishikawa
Summary: Asymmetric total syntheses of truncated derivatives of the aplysiatoxin/oscillatoxin family of marine natural products, aplysiaenal (1) and nhatrangin A (2), using configurationally defined intermediates are reported. The NMR spectra of our synthesized nhatrangin A did not match with authentic samples or material obtained via two other total syntheses, but were similar to a sample obtained in a third total synthesis. By independently synthesizing the fragments used in its total syntheses, we confirmed the configuration of nhatrangin A and clarified that the spectroscopic discrepancy is due to salt formation of the carboxylic acid moiety.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Neurosciences
Jiangshan Zhan, Florian Nepomuk Fegg, Hannes Kaddatz, Sebastian Ruehling, Julia Frenz, Bernd Denecke, Sandra Amor, Peter Ponsaerts, Tanja Hochstrasser, Markus Kipp
Summary: This study found that acute demyelinating white matter CNS lesion led to ongoing axonal degeneration and glia cell activation despite normal myelination and oligodendrocyte densities in the remyelinated corpus callosum. High-speed ventral plane videography revealed subtle gait abnormalities in the affected mice.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Cell Biology
Rulu Pan, Juji Dai, Weicheng Liang, Hongxiao Wang, Lin Ye, Siqi Ye, Ziqi Lin, Shishun Huang, Yan Xiong, Li Zhang, Liting Lu, Ouchen Wang, Xian Shen, Wanqin Liao, Xincheng Lu
Summary: Phosphodiesterase 4D interacting protein (PDE4DIP) is found to be commonly mutated in human cancers and plays an oncogenic role in colorectal cancer (CRC) growth and adaptive MEK inhibitor (MEKi) resistance. PDE4DIP upregulation is associated with poor prognosis in CRC patients and its knockdown impairs the growth of KRAS-mutant CRC cells by inhibiting the core RAS signaling pathway. Mechanistically, PDE4DIP promotes the degradation of the RAS GTPase-activating protein (RasGAP) neurofibromin (NF1) and reactivates the RAS/ERK pathway, leading to adaptive MEKi resistance in KRAS-mutant CRC.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Hai-Liang Zhang, Bing-Xin Hu, Zhi-Ling Li, Tian Du, Jia-Lu Shan, Zhi-Peng Ye, Xiao-Dan Peng, Xuan Li, Yun Huang, Xian-Ying Zhu, Yu-Hong Chen, Gong-Kan Feng, Dajun Yang, Rong Deng, Xiao-Feng Zhu
Summary: Through CRISPR-Cas9 and kinase inhibitor screening, Zhang et al. found that PKC beta II phosphorylates and activates ACSL4 to enhance polyunsaturated fatty acid-containing lipid biosynthesis, thereby promoting accumulation of lipid peroxidation and ferroptosis.
NATURE CELL BIOLOGY
(2022)
Review
Gastroenterology & Hepatology
Kohji Yamada, Kiyotsugu Yoshida
Summary: Protein kinase C delta (PKC delta) is a member of the PKC family and plays important roles in various biological and cancerous processes. Its function is regulated by factors such as phosphorylation and subcellular localization. Recent studies have found its unique extracellular localization and growth factor-like function in liver cancer cells, contributing to our understanding of its involvement in liver cancer progression.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Chemistry, Multidisciplinary
Veronika M. Shoba, Dhanushka N. P. Munkanatta Godage, Santosh K. Chaudhary, Arghya Deb, Sachini U. Siriwardena, Amit Choudhary
Summary: Phosphorylation-inducing chimeric small molecules (PHICS) can enable protein kinase C (PKC) to have new activities, such as inducing inhibitory neo-phosphorylations on a variant and phosphorylating cytosol-based neo-substrates. PKC with PHICS can also induce death of chronic myeloid leukemia cells. Synthetic chemistry has the power to expand the chemical and functional diversity of proteins in cells using bifunctional molecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Physical
Huan Liu, Xiuxia Su, Jing Zhang, Jia Xu, Dong Yang, Qingcai Chen
Summary: The study demonstrated a rapid PKC detection method based on liquid crystal biosensor and nano-modified signal amplification technology, achieving sensitive detection of PKC with good linear relationship and a very low detection limit in a wide linear range.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Biology
Ranran Shi, Yubing Li, Ling Ran, Yu Dong, Xiuman Zhou, Jingwen Tang, Lu Han, Mingshuang Wang, Liwei Pang, Yuanming Qi, Yahong Wu, Yanfeng Gao
Summary: Researchers identified three HLA-A2-restricted neoepitopes that could induce specific antitumor T cell responses in non-MSI-H colorectal cancer, showing promise for potential immunotherapy. The combination of adoptive T cell therapy based on these neoepitopes with immune checkpoint inhibitors, such as anti-PD-1, could provide a promising treatment strategy for non-MSI-H colorectal cancer.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Chemistry, Medicinal
Chen Ling, Xiuman Zhou, Yanfeng Gao, Xinghua Sui
Summary: This study identified two distinct subtypes of esophageal adenocarcinoma (EAC) with significant prognostic differences and differences in tumor microenvironments. The findings suggest that EAC patients of subtype 2 may benefit from PD-1 blockade therapy, while patients of subtype 1 are more responsive to chemotherapy or combination therapy.
Article
Automation & Control Systems
Jianbo Yu, Chaoang Xiao, Tianzhong Hu, Yanfeng Gao
Summary: This paper introduces a new multi-scale morphological filtering method called selective weighted multi-scale morphological filter (SWMMF). This method combines filtering results of different scales based on an adaptive weighting strategy to enhance the extraction of faulty components. The effectiveness of this approach is proven by comparing it with single-scale and average weighting filters on simulation and real-world cases (bearing vibration signals with different defects).
Article
Materials Science, Multidisciplinary
Wenhu Xu, Jin Yang, Mingjian Peng, Yixuan Zhao, Hongbin Liu, Peiran Deng, Yanfeng Gao, Hua Zhang
Summary: This study proposed a torch-offset CMT-CS welding method for joining different aluminum alloys and copper. The experiments showed that the offset distance had a significant influence on the weld heat input and the wettability of the filler material. Moreover, changing the joint configuration led to variations in the mechanical properties and fracture modes of the weld.
JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
(2023)
Article
Chemistry, Physical
Jiadong Zhou, Chao Zhu, Yao Zhou, Jichen Dong, Peiling Li, Zhaowei Zhang, Zhen Wang, Yung-Chang Lin, Jia Shi, Runwu Zhang, Yanzhen Zheng, Huimei Yu, Bijun Tang, Fucai Liu, Lin Wang, Liwei Liu, Gui-Bin Liu, Weida Hu, Yanfeng Gao, Haitao Yang, Weibo Gao, Li Lu, Yeliang Wang, Kazu Suenaga, Guangtong Liu, Feng Ding, Yugui Yao, Zheng Liu
Summary: This study reveals a competitive-chemical-reaction-based growth mechanism that allows for the growth of transition metal chalcogenides and transition metal phosphorous chalcogenides with different compositions and phases. It provides an interesting platform for the exploration of 2D TMPCs and TMCs.
Article
Oncology
Ranran Shi, Xiuman Zhou, Liwei Pang, Mingshuang Wang, Yubing Li, Chunxia Chen, Haoming Ning, Lihan Zhang, Guangxing Yue, Lu Qiu, Wenshan Zhao, Yuanming Qi, Yahong Wu, Yanfeng Gao
Summary: The majority of non-microsatellite instability-high colorectal cancers are immunologically cold due to poor CD8(+) T cell infiltration. The discovered cancer-testis antigen (CTA) ODF2 is negatively correlated with CD8(+) T cell infiltration and can improve CTA infiltration through upregulating interleukin-15.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Nanoscience & Nanotechnology
Yanfeng Gao, Dongtao Zhou, Qin Xu, Jingjing Li, Wen Luo, Jingjing Yang, Yongchun Pan, Ting Huang, Yanping Wang, Bangshun He, Yujun Song, Yuzhen Wang
Summary: In this study, a fluorescent bacteria counting immunoassay (FBCIA) strategy was developed for ultrasensitive quantification of protein biomarkers. The strategy involved a cascade signal conversion and amplification strategy, which enabled the conversion of target protein concentration to countable bacterial number. The developed platform demonstrated highly sensitive detection of CEA and PSA with low detection limits, and a smartphone device was also developed for point-of-care counting. The good consistency between FBCIA and clinical ELISA validated the high reliability and promising potential of the platform in clinical applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Applied
Xiyuan Zhang, Yanfeng Gao, Zhiwei Miao
Summary: Palladium-catalyzed asymmetric [4+3] cycloadditions of indene-2-carbaldehydes and 4-vinyl benzoxazinanones proceeded smoothly in the presence of chiral phosphine ligands to give the corresponding functionalized 5H-benzo[b]azepines (26 examples, 60% to 89% yield, 88:12 to 95.5:4.5 er) under mild conditions.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Chemistry, Analytical
Yanfeng Gao, Yanping Wang, Xinli Liu, Zhenxing Zhu, Zhun Li, Zhibin Zhang, Yi Yin, William Chi Shing Cho, Yujun Song, Yuzhen Wang
Summary: We developed a signal amplification method based on streamlined click reactions to immobilize large amounts of hemin on graphene oxide nanosheets, allowing for highly sensitive detection of various biological targets. The designed platform shows great promise for graphene-based nanozymes and sensitive biosensing in a wider range of applications.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Wen Su, Mixiao Tan, Zhihang Wang, Jie Zhang, Wenping Huang, Haohao Song, Xinye Wang, Haitao Ran, Yanfeng Gao, Guangjun Nie, Hai Wang
Summary: Proteolysis targeting chimeras (PROTACs) technology is a promising method for degrading disease-associated proteins. In this study, carbon-dot (CD)-based PROTACs (CDTACs) were developed to degrade membrane proteins via the ubiquitin-proteasome system. CDTACs demonstrated efficient degradation of programmed cell death ligand 1 (PD-L1) and activation of the stimulator of interferon genes (STING) pathway, leading to the inhibition of tumor growth. CDTACs offer advantages such as targeted tumor accumulation, immune system activation, and in vivo detection compared to small-molecule-based PROTACs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Fisheries
Shiyong Yang, Weikai Sui, Xiaoyu Ren, Xiaoyu Wang, Guixian Bu, Fengyan Meng, Xiaohan Cao, Guozhi Yu, Xingfa Han, Anqi Huang, Qiuxia Liang, Jiayun Wu, Yanfeng Gao, Xiuhong Wang, Xianyin Zeng, Xiaogang Du, Yunkun Li
Summary: In this study, the TLR18 and UNC93B1 genes were cloned from Schizothorax prenanti. It was found that TLR18 can activate immune response through NF-kappa B and type I IFN pathways in teleost fishes, and UNC93B1 positively regulates TLR18-mediated NF-kappa B signal.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Engineering, Industrial
Jianbo Yu, Shijin Li, Xing Liu, Yanfeng Gao, Shijin Wang, Changhui Liu
Summary: This paper proposes a new method, DCGRUA-AE, for fault detection in dynamic industrial processes. The method integrates CGRU, attention mechanism, and auto-encoder to learn both global and local features of dynamic process data. The feasibility and superiority of the method have been verified through experiments on four industrial processes.
INTERNATIONAL JOURNAL OF PRODUCTION RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Yongchun Pan, Xiaowei Luan, Yanfeng Gao, Fei Zeng, Xuyuan Wang, Dongtao Zhou, Wanqi Li, Yuzhen Wang, Bangshun He, Yujun Song
Summary: Researchers have designed a cancer-activated nanomachine based on porous upconversion nanoparticles for precise phototherapy. The nanosystem contains a telomerase substrate primer and encapsulates 5-aminolevulinic acid and D-arginine. After coating with hyaluronic acid, the nanomachine can easily enter tumor cells and induce efficient accumulation of protoporphyrin IX via biosynthetic pathway. The nanomachine can respond to near-infrared light and generate active singlet oxygen through Förster resonance energy transfer. Moreover, it can relieve tumor hypoxia by converting D-arginine into nitric oxide, thereby improving the phototherapy effect.
Article
Oncology
Xinghua Sui, Chunxia Chen, Xiuman Zhou, Xueyan Wen, Chao Shi, Guanyu Chen, Juan Liu, Zhuoying He, Yongjie Yao, Yin Li, Yanfeng Gao
Summary: This study integrated single-cell transcriptomic sequencing and large microarray sequencing to investigate key cell subtypes and therapeutic targets in esophageal squamous cell carcinoma (ESCC). The researchers discovered that the chemokine CCL18 secreted by tumor associated macrophages promotes tumor cell proliferation through the JAK2/STAT3 signaling pathway and leads to poor prognosis of ESCC. CCL18 blockade was found to significantly prevent the progression of ESCC, providing novel therapeutic targets and strategies for ESCC treatment.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Chemistry, Analytical
Yuanyue Han, Xinli Liu, Qiao Zhao, Yanfeng Gao, Dongtao Zhou, Wenxiu Long, Yuzhen Wang, Yujun Song
Summary: A simple aptazyme-induced cascade signal amplification (ACSA) was integrated with a triple-channel volumetric bar-chart chip (TV-Chip) for the visual quantification of aflatoxin B1 (AFB1) and adenosine triphosphate (ATP). Bifunctional aptazymes modified on magnetic silica nanoparticles (MSNPs) were used as sensing probes. The detection limits of AFB1 and ATP were easily achieved without additional instruments. The results were in good agreement with a commercial AFB1 ELISA kit, showing the accuracy and reliability of this method. The ACSA-based TV-Chip holds great promise for on-site and real-time quantitation of trace targets.
