Article
Microbiology
Yuan Luo, Jing Wang, Yi-Lin Gu, Li-Qun Zhang, Hai-Lei Wei
Summary: This study found that the two flagellins (FliC-1 and FliC-2) in Pseudomonas kilonensis F113 are evolutionarily distinct, with only FliC-1 contributing to bacterial activity and plant immune responses. The lower expression level of FliC-2 may be due to variations in promoter activity. The study also found that FliC-1 induces stronger plant immune responses and inhibits plant root growth compared to FliC-2.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Xiaolin Liu, Anna Roujeinikova, Karen M. Ottemann
Summary: FliL is a protein found in the flagellar motor of bacteria. It was previously unclear what role it plays. However, through reanalyzing studies on FliL, scientists discovered that the presence or absence of different regions of the protein affects motility. The N-terminal region is found to have a negative effect on motor function, and its removal allows normal motility.
Article
Plant Sciences
Noel Djitro, Rebecca Roach, Rachel Mann, Brendan Rodoni, Cherie Gambley
Summary: This study reports the first identification of Pseudomonas syringae strains infecting zucchini in Australia, causing significant yield losses. The isolates exhibited variations in pathogenicity across different plant species and some produced fluorescent pigment. Additionally, it was discovered that different zucchini varieties showed varying levels of susceptibility to the isolates.
Article
Biology
Weijie Chen, Neha Mani, Hamid Karani, Hao Li, Sridhar Mani, Jay X. Tang
Summary: In this study, by confining bacterial movement in circular microwells, it was found that swarming bacteria and planktonic bacteria exhibited different collective motion patterns within a specific size range. The observation of 'rafting behavior' of swarming bacteria upon dilution suggests a potential mechanism for the differences in motion patterns. The experimental design using PDMS microchip disk arrays enabled the observation of bacterial swarming on murine intestinal surface, providing a new method for characterizing bacterial swarming in complex environments.
Article
Biochemistry & Molecular Biology
Ixchell Y. Sedillo-Torres, alvaro O. Hernandez-Rangel, Yolanda Gomez-y-Gomez, Daniel Cortes-Avalos, Blanca Estela Garcia-Perez, Juan C. Villalobos-Rocha, Cesar H. Hernandez-Rodriguez, Luis Gerardo Zepeda-Vallejo, Paulina Estrada-de los Santos, Maria Elena Vargas-Diaz, Jose Antonio Ibarra
Summary: An organic extract of Hibiscus sabdariffa L. containing hibiscus acid was found to inhibit motility and reduce the secretion of virulence factors in Salmonella enterica serovars Typhi and Typhimurium. The compound was not toxic to eukaryotic cells and could reduce the invasion of Salmonella Typhimurium in epithelial cells. Initial studies suggested that the mode of action of hibiscus acid might involve the disruption of membrane proton balance.
Review
Biochemistry & Molecular Biology
Mathilde Bouteiller, Charly Dupont, Yvann Bourigault, Xavier Latour, Corinne Barbey, Yoan Konto-Ghiorghi, Annabelle Merieau
Summary: Flagella-driven motility is crucial for bacterial colonization and virulence, with differences in flagellar structure and assembly between enteric bacteria and Pseudomonads. Understanding the specificities of flagellar genes expression and assembly processes helps to elucidate the differences in flagella functions among different bacterial species. Additionally, the fuel utilized for flagellar production and rotation, as well as known regulatory pathways, play important roles in the overall functionality of the flagellar macromolecular complex.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Plant Sciences
Jian Chen, Rouhallah Shari, Choong-Min Ryu
Summary: Sieber et al. discovered a new volatile signaling molecule, leudiazen, and showed that inactivating it can reduce the pathogenicity of Pseudomonas synngae pv. synngae, providing a new plant protection strategy compatible with organic farming.
TRENDS IN PLANT SCIENCE
(2022)
Article
Microbiology
Marek Bogacz, Faiha M. El Abbar, Claudia A. Cox, Jiaqi Li, Jarred S. Fiedler, Lynn K. H. Tran, Paul M. H. Tran, C. Luke Daugherty, Kate H. Blake, Zhirui Wang, Parastoo Azadi, Stuart A. Thompson
Summary: The study identified the FliW binding site on CsrA in Campylobacter jejuni and found that specific mutations (V51A and N55A) significantly decreased FliW binding. These mutations, as well as the conservation of certain residues in bacterial species, suggest a potential regulation mechanism by FliW in bacterial CsrA proteins.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Wenlu Zhang, Shasha Li, Bo Deng, Pengfei Wu, Yingbin Shui, Jiaming Zhang, Zhongjie Li
Summary: This study used RNA-sequencing to analyze sRNAs in antibiotic-resistant PAO1, finding a large number of differentially expressed sRNAs. Target gene prediction and pathway analysis revealed the potential roles of these differentially expressed sRNAs in regulating antibiotic resistance.
MICROBIAL PATHOGENESIS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Elena A. Zehr, Antonina Roll-Mecak
Summary: Two separate studies have reported high-resolution structures of the almost complete central apparatus, providing insights into the mechanism of ciliary beating and advancing our molecular understanding of cilia architecture and function.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cynthia Alias-Villegas, Francisco Fuentes-Romero, Virginia Cuellar, Pilar Navarro-Gomez, Maria J. Soto, Jose-Maria Vinardell, Sebastian Acosta-Jurado
Summary: Bacteria can spread on surfaces to colonize new environments. Flavonoids and bacterial transcriptional activator NodD have an impact on the surface motility of Sinorhizobium fredii HH103.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Marina Anteljevic, Iva Rosic, Olja Medic, Stoimir Kolarevic, Tanja Beric, Slavisa Stankovic, Ivan Nikolic
Summary: This study identified diverse strains of Pseudomonas syringae in the Danube River Basin, with potential to cause disease outbreaks on crops. It provides valuable insights for future studies and monitoring of population dynamics.
PHYTOPATHOLOGY RESEARCH
(2023)
Article
Chemistry, Physical
R. Schwidetzky, P. Sudera, A. T. Backes, U. Poeschl, M. Bonn, J. Froehlich-Nowoisky, K. Meister
Summary: Research shows that ice-nucleating proteins alone are not enough to achieve maximum freezing efficiency. Intact cell membranes play a critical role, and components like phospholipids and lipopolysaccharides enable the assembly of INPs. Additionally, the size and level of order/disorder in these assemblies are crucial in determining bacterial INPs' ability to facilitate ice nucleation.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Gastroenterology & Hepatology
Dominika Trzilova, Mercedes A. H. Warren, Nicole C. Gadda, Caitlin L. Williams, Rita Tamayo
Summary: C. difficile exhibits heterogeneity in swimming motility and toxin production through phase variation by site-specific DNA recombination. The ability of C. difficile to phase vary flagella and toxins influences colonization and disease development, indicating that the phenotypic variants generated by flagellar switch inversion have distinct capacities for causing disease.
Article
Microbiology
Yunjie Chang, Hui Xu, Md A. Motaleb, Jun Liu
Summary: Researchers have discovered a multiprotein collar complex in Lyme disease spirochetes, which exhibits remarkable structural plasticity to accommodate the highly curved membrane and generate the high torque necessary for spirochete motility.
Article
Microbiology
Caroline L. Monteil, David Vallenet, Nicolas Menguy, Karim Benzerara, Valerie Barbe, Stephanie Fouteau, Corinne Cruaud, Magali Floriani, Eric Viollier, Geraldine Adryanczyk, Nathalie Leonhardt, Damien Faivre, David Pignol, Purificacion Lopez-Garcia, Richard J. Weld, Christopher T. Lefevre
NATURE MICROBIOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Caroline L. Monteil, Christopher T. Lefevre
TRENDS IN MICROBIOLOGY
(2020)
Review
Microbiology
Matthieu Amor, Francois P. Mathon, Caroline L. Monteil, Vincent Busigny, Christopher T. Lefevre
ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Ecology
Caroline L. Monteil, Denis S. Grouzdev, Guy Perriere, Beatrice Alonso, Zoe Rouy, Stephane Cruveiller, Nicolas Ginet, David Pignol, Christopher T. Lefevre
Article
Multidisciplinary Sciences
Noa Keren-Khadmy, Natalie Zeytuni, Nitzan Kutnowski, Guy Perriere, Caroline Monteil, Raz Zarivach
Editorial Material
Biochemistry & Molecular Biology
Dirk Schueler, Caroline L. Monteil, Christopher T. Lefevre
TRENDS IN MICROBIOLOGY
(2020)
Article
Ecology
Caroline L. Monteil, Karim Benzerara, Nicolas Menguy, Cecile C. Bidaud, Emmanuel Michot-Achdjian, Romain Bolzoni, Francois P. Mathon, Margot Coutaud, Beatrice Alonso, Camille Garau, Didier Jezequel, Eric Viollier, Nicolas Ginet, Magali Floriani, Sufal Swaraj, Martin Sachse, Vincent Busigny, Elodie Duprat, Francois Guyot, Christopher T. Lefevre
Summary: Bacteria can synthesize a variety of non-biological precipitates with different chemical compositions and structures within cells, including magnetic particles and calcium carbonate granules. Research has shown that the amorphous calcium carbonate granules formed by these bacteria are contained within membrane-delimited vesicles. Analysis of a new genus of Alphaproteobacteria revealed atypical structure of the magnetosome gene cluster, and the bacteria consume energy to maintain an environment suitable for calcium carbonate granule formation.
Article
Biochemistry & Molecular Biology
Nir Hecht, Caroline L. Monteil, Guy Perriere, Marina Vishkautzan, Eyal Gur
Summary: This study investigates the differences in functionality between two homologous bacterial enzymes, PafA and Dop, by analyzing their amino acid sequences and structural elements. Through reciprocal mutagenesis, researchers were able to alter the activity of the enzymes and identify key residues and structural components responsible for their unique functions. The study demonstrates the evolutionary pathways that led to the emergence of novel catalytic functions in these enzymes.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biology
Karim Benzerara, Romain Bolzoni, Caroline Monteil, Olivier Beyssac, Olivier Forni, Beatrice Alonso, Maria P. Asta, Christopher Lefevre
Summary: The study on Achromatium cells revealed that the intracellular CaCO3 granules are predominantly composed of amorphous calcium carbonate (ACC), similar to other cyanobacteria and a recently discovered magnetotactic alphaproteobacterium. The physiological and ecological implications of this finding are worth further investigation. Future studies on the crystallinity of CaCO3 granules in Achromatium cells from diverse environments worldwide should be cautious of potential pitfalls identified in this study.
Article
Microbiology
Vincent Busigny, Francois P. Mathon, Didier Jezequel, Cecile C. Bidaud, Eric Viollier, Gerard Bardoux, Jean-Jacques Bourrand, Karim Benzerara, Elodie Duprat, Nicolas Menguy, Caroline L. Monteil, Christopher T. Lefevre
Summary: Studying magnetotactic bacteria in Lake Pavin, utilizing various sampling methods, understanding their ecological niche, and efficiently collecting large volumes of water samples using an online pumping system. This research provides a foundation for further multidisciplinary investigations of magnetotactic bacteria populations in Lake Pavin and elsewhere, as well as chemical and isotopic analyses of their magnetosomes.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Deepa Paliwal, Amanda J. Hamilton, Glyn A. Barrett, Fabrizio Alberti, Helmut van Emden, Caroline L. Monteil, Tim H. Mauchline, Ralf Nauen, Carol Wagstaff, Chris Bass, Robert W. Jackson
Summary: Environmental bacteria have been found to have the ability to kill aphids, offering an alternative and sustainable control measure. Pseudomonas fluorescens PpR24 showed the highest toxicity against various aphid clones and could survive on plants for over three weeks, resulting in a significant reduction in aphid populations.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Ecology
Kevin C. Failor, Haijie Liu, Marco E. Mechan Llontop, Sophie LeBlanc, Noam Eckshtain-Levi, Parul Sharma, Austin Reed, Shu Yang, Long Tian, Christopher Lefevre, Nicolas Menguy, Liangcheng Du, Caroline L. Monteil, Boris A. Vinatzer
Summary: Earth's radiation budget and precipitation are impacted by aerosols containing ice nucleation activity (INA), involving bacteria, fungi, and pollen. This study identified Lysinibacillus parviboronicapiens (Lp) as the first Gram-positive bacterium with INA, which is associated with a unique macromolecule or particle. A combination of genomics, transcriptomics, and mutant screening revealed that INA in Lp relies on a polyketide synthase and a peptide synthetase. These findings advance our understanding of biological ice nucleation and may serve as a model for other organisms with unknown INA mechanisms.
Article
Microbiology
Denis Trubitsyn, Caroline L. Monteil, Corey Geurink, Viviana Morillo-Lopez, Luiz Gonzaga Paula de Almeida, Ana Tereza Ribeiro de Vasconcelos, Fernanda Abreu, Dennis A. Bazylinski, Christopher T. Lefevre
Summary: The 3.7-Mb genome sequence of strain SS-5 provides further diversity for understanding the origin and evolution of magnetotaxis and magnetosome biomineralization.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
Corey Geurink, Christopher T. Lefevre, Caroline L. Monteil, Viviana Morillo-Lopez, Fernanda Abreu, Dennis A. Bazylinski, Denis Trubitsyn
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Meeting Abstract
Plant Sciences
C. Morris, O. Berge, C. Chandeysson, C. Guilbaud, C. Monteil, P. Darwin, A. Sigurbjornsdottir, O. Vilhelmsson