Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 30, Issue 4, Pages 2367-2378Publisher
SPRINGER
DOI: 10.1007/s10811-018-1443-0
Keywords
Marine microalga; Pavlova lutheri; Matrix metalloproteinase-9 (MMP-9); Nuclear factor-kappa B (NF-kappa B); Mitogen-activated protein kinases (MAPKs)
Funding
- National Research Foundation (NRF) - Ministry of Education, Science, and Technology [2015R1D1A1A01061334]
- Marine Biotechnology Program - Ministry of Oceans and Fisheries of Korean Government [20170326]
- Korea Institute of Ocean Science Technology [PE99621]
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In this study we isolate a heptameric peptide from the marine microalga Pavlova lutheri and examine its inhibitory effect and mechanism of action on the matrix metalloproteinase-9 (MMP-9) protein in human fibrosarcoma HT1080 cells. Through consecutive rounds of purification we isolated a potent MMP-9 inhibitory peptide composed of seven amino acids: Leu-Leu-Ala-Pro-Pro-Glu-Arg (796.4 Da). We show that the mRNA and protein expression levels of MMP-9 are reduced in the presence of this purified peptide. Furthermore, we show that this peptide inhibits PMA-induced translocation of nuclear factor-kappa B (NF-kappa B) by suppressing I kappa B-alpha phosphorylation and that it suppresses the phosphorylation of the c-Jun N-terminal (JNK) and p38 mitogen-activated protein kinases (MAPKs). Our results indicate that this peptide, purified from P. lutheri, has the potential to be a valuable natural preventive agent against MMP-related diseases.
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