Journal
GENETICS AND MOLECULAR RESEARCH
Volume 14, Issue 2, Pages 3984-3994Publisher
FUNPEC-EDITORA
DOI: 10.4238/2015.April.27.13
Keywords
bEnd.3; Cell adhesion molecule; Fimbristylis ovata; U937; Proinflammatory cytokines; Random activation of gene expression
Funding
- Chulalongkorn University
- Center of Excellence in Omics-Nanomedical Technology Development Project
Ask authors/readers for more resources
Fimbristylis ovata has been long used as a traditional medicine for chronic inflammatory diseases; however, there are no data regarding its anti-inflammatory properties. In this study, we investigated the effects of F. ovata extracts on the secretion of pro-inflammatory cytokines, cell adhesion molecule, and receptor for advanced glycation end-products (RAGE) in lipopolysaccharide-stimulated cells. F. ovata was extracted using the maceration method with 3 different solvents: ethanol, methanol, and water. The effect of F. ovata extracts on cell viability was evaluated using the MTT assay. Pro-inflammatory cytokines and cell adhesion molecules were investigated by reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay. Upon incubation with F. ovata extracts up to 100 mg/mL, cell viability was more than 80%. F. ovata extracts could inhibit interleukin-6 level and gene expression as well as the RAGE gene in the monocytic cell lineU937. Moreover, the results showed that vascular cell adhesion molecule 1 secretion and gene expression were decreased when lipopolysaccharide-activated brain endothelial cells (bEnd.3) were treated with F. ovata extracts. Therefore, the anti-inflammatory activity of F. ovata extracts may result from their inhibitory actions via the RAGE signaling pathway.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available