Article
Agriculture, Dairy & Animal Science
Lechao Zhang, Chunhui Duan, Yunxia Guo, Yingjie Zhang, Yueqin Liu
Summary: The inhibition of PRL by bromocriptine promotes secondary hair follicle development in the anagen phase, possibly by downregulating MTNR1a and up-regulating ROR alpha gene expression in the skin.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Genetics & Heredity
Mengli Zhao, Huitong Zhou, Yuzhu Luo, Jiqing Wang, Jiang Hu, Xiu Liu, Shaobin Li, Kaiwen Zhang, Huimin Zhen, Jon G. H. Hickford
Summary: The KRTAP1-2 gene in goats has been identified and found to have multiple sequence variants that are associated with raw cashmere fiber weight. However, these variants do not affect fiber diameter and length.
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Biochemistry & Molecular Biology
Krisztina Nikovics, Cedric Castellarin, Xavier Holy, Marjorie Durand, Halima Morin, Abdelhafid Bendahmane, Anne-Laure Favier
Summary: Understanding bone homeostasis and regeneration is of great clinical and scientific importance for the development of bone therapy. Macrophages play a crucial role in bone regeneration during healing. In situ hybridization and hybridization chain reaction can be used to explore gene expression in bone tissues.
Article
Genetics & Heredity
Emma Sicherre, Anne-Laure Favier, Diane Riccobono, Krisztina Nikovics
Summary: The study found that different antibodies for investigating CD206 expression may result in varied expression patterns during immunodetection, potentially leading to false results. Therefore, researchers need to carefully choose methods for characterizing macrophages. HCR is shown to be an excellent alternative method for detecting macrophages in situ.
Article
Infectious Diseases
Sheila B. Buijs, Jesper M. Weehuizen, Tim K. Jensen, Mette Boye, Mirjam H. A. Hermans, Peet T. G. A. Nooijen, Andy IM. Hoepelman, Chantal P. Bleeker-Rovers, Jan Jelrik Oosterheert, Peter C. Wever
Summary: This study assesses the value of fluorescence in situ hybridization (FISH) for detecting Coxiella burnetii in tissue samples from chronic Q fever patients. The results indicate that FISH has limited sensitivity and specificity, and its value in routine diagnostics for chronic Q fever is limited.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Biochemical Research Methods
Ian Dardani, Benjamin L. Emert, Yogesh Goyal, Connie L. Jiang, Amanpreet Kaur, Jasmine Lee, Sara H. Rouhanifard, Gretchen M. Alicea, Mitchell E. Fane, Min Xiao, Meenhard Herlyn, Ashani T. Weeraratna, Arjun Raj
Summary: clampFISH 2.0 is an efficient method for detecting multiple RNA species and amplifying signals simultaneously, enabling the detection of 10 different RNA species in over 1 million cells and also applicable to tissue sections.
Article
Parasitology
Gereon Schares, Majda Globokar Vrhovec, Mareen Tuschy, Maike Joeres, Andrea Baerwald, Bretislav Koudela, Jitender P. Dubey, Pavlo Maksimov, Franz J. Conraths
Summary: The 529-bp repeat of H. hammondi can be used to establish a quantitative real-time PCR assay, with excellent specificity and sensitivity. This assay is a valuable diagnostic tool for epidemiological and cell biological studies on H. hammondi and related parasites.
PARASITES & VECTORS
(2021)
Article
Multidisciplinary Sciences
Dina M. Metwally, Reem Alajmi, Muslimah N. Alsulami, Isra M. Al-Turaiki, Rewaida Abdel-Gaber, Afrah F. Alkhuriji, Haleema H. Albohiri, Khalil Mohamed, Hanadi B. Baghdadi, Manal F. El-Khadragy, Guillermo T. Isaias, Saeed El-Ashram
Summary: The study found that Theileria spp. were detected in 57.8% of sheep and 51.9% of goats in Jeddah, Saudi Arabia. Phylogenetic analysis of the sequence data revealed that the T. ovis identified in this study were closely related to an isolate from Turkey, with pairwise identity ranging from 84.4% to 99.8% and coverage ranging from 52.35% to 99.79%.
Article
Biochemistry & Molecular Biology
Mei Yang, Ruihua Ji, Zhengqing Zhao, Wenwen Wang, Ye Lu, Zhenghua Xiang, Hongbin Yuan
Summary: This study established a new method called In Situ Hybridization Chain Reaction (isHCR) for in situ detection of apoptotic DNA fragments. Compared to the current TUNEL method, isHCR can specifically detect DNA fragmentation in the cytoplasm of early-stage apoptotic cells, with low cost and the ability to handle large numbers of specimens. This suggests that isHCR has the potential to play a role in the in situ detection of DNA fragmentation in apoptotic cells.
Article
Chemistry, Analytical
Jianhui Xu, Ruijin Zeng, Lingting Huang, Zhenli Qiu, Dianping Tang
Summary: A magnetic-assisted platform was established for monitoring prostate-specific antigen (PSA), based on Bi2MoO6 nanosheets (BMO) and coupling the aptamer-guided hybridization chain reaction (HCR) with the hydrolysate-induced vulcanization reaction. The platform showed high precision with a quantitative diagnosis sensitivity area in a range of 5.0 pg mL(-1)-100 ng mL(-1).
ANALYTICAL CHEMISTRY
(2022)
Article
Optics
Damian Presti, Julian Bergier, Lucas Ripoll, Cristina Borio, Gustavo A. Torchia, Marcos Bilen
Summary: Since the global pandemic of SARS-CoV-2, the need for a low-cost and faster diagnostic tool for evaluating symptomatic patients has emerged. Point-of-care testing and point-of-need testing systems have been developed to provide accurate and rapid diagnostics. In this study, a bio-photonic device using an iso-thermal system was developed for detecting SARS-CoV-2. The device showed comparable sensitivity to the commercially used reference method and was built with simple and low-cost components, making it a high-efficiency and affordable instrument.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Chemistry, Multidisciplinary
Bowen Zhang, Taoran Tian, Dexuan Xiao, Shaojingya Gao, Xiaoxiao Cai, Yunfeng Lin
Summary: A DNA-enhanced HCR detection system (T-probe system) is introduced for the detection of cancer-related targets, demonstrating its versatility and efficiency by detecting miRNA-21 and nucleolin. The T-probe system shows high detection rate and rapid recognition of both membrane and intracellular cancer-related targets, and exhibits superior fluorescence for imaging tumor-related biomarkers in vitro and in vivo, indicating its great potential for clinical diagnosis and therapeutic applications.
ADVANCED FUNCTIONAL MATERIALS
(2022)
