Article
Microbiology
Ola Alessa, Yoshitoshi Ogura, Yoshiko Fujitani, Hideto Takami, Tetsuya Hayashi, Nurettin Sahin, Akio Tani
Summary: This study used a comprehensive comparative genomics approach to reveal the phylogenetic relationships among PPFMs and explain the genotypic differences that confer their different phenotypes. The research found that methylotrophy, nitrate utilization, and anoxygenic photosynthesis are distinguishing features of PPFMs from other members of Methylobacteriaceae. Additionally, some strains lack MxaF-type methanol dehydrogenase but possess XoxF-type MDH enabling growth on methanol in the presence of lanthanum.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Agnieszka Daszczynska, Tomasz Krucon, Robert Stasiuk, Marta Koblowska, Renata Matlakowska
Summary: This study investigated the occurrence and diversity of proteobacterial XoxF-type methanol dehydrogenases (MDHs) in a microbial community inhabiting a fossil organic matter- and sedimentary lanthanide-rich underground mine environment. The results showed that XoxF-type MDHs were produced by different bacteria from Alpha-, Beta-, and Gammaproteobacteria, with Nitrosomonadales and Rhizobiales having the highest presence of XoxF-encoding genes and matching protein sequences. The study also confirmed the accumulation of light lanthanides and the presence of methanol in the microbial mat. This research expands our understanding of XoxF-producing bacteria and the distribution and diversity of these enzymes in natural environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Bruno Maucourt, David Roche, Pauline Chaignaud, Stephane Vuilleumier, Francoise Bringel
Summary: This study provides the first genome-wide landscape of transcription start sites (TSS) for methylotrophic bacteria using DCM or methanol as carbon and energy sources. The results suggest that DCM biotransformation has the potential to be a sustainable decontamination strategy. Additionally, investigating the transcriptional regulation mechanisms in bacteria grown on DCM or methanol is crucial for future research in this field.
Article
Microbiology
Paula Roszczenko-Jasinska, Tomasz Krucon, Robert Stasiuk, Renata Matlakowska
Summary: This study identified the presence of XoxF methanol dehydrogenases in a bacterial community dominated by Proteobacteria inhabiting shale rock, with the enzyme being produced by bacterial orders such as Rhizobiales, Rhodobacterales, Rhodospiralles, Burkholderiales, and Nitrosomonadales. The discovery of XoxF proteins in the metaproteome of lithobiontic bacterial communities colonizing underground rocks rich in light lanthanides expands our understanding of the ecology and diversity of these enzymes in natural environments.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xiao-Jie Yuan, Wen-Jing Chen, Zeng-Xin Ma, Qian-Qian Yuan, Min Zhang, Lian He, Xu-Hua Mo, Chong Zhang, Chang-Tai Zhang, Meng-Ying Wang, Xin-Hui Xing, Song Yang
Summary: By introducing the RuMP cycle and a synergistic assimilation pathway, growth rate and methanol consumption rate of M. extorquens AM1 can be increased, along with the production of 3-HP. This strategy improves the efficiency of central methane metabolism by adjusting gene transcription.
METABOLIC ENGINEERING
(2021)
Article
Biochemical Research Methods
Zeng-xin Ma, Min Zhang, Chang-tai Zhang, Hui Zhang, Xu-hua Mo, Xin-hui Xing, Song Yang
Summary: The study investigated the potential of using pyruvate to produce isobutanol in engineered Methylorubrum extorquens AM1. By optimizing the metabolic pathways and utilizing an isobutanol-tolerant strain, the production of isobutanol was significantly increased, showcasing the feasibility of converting methanol to valuable chemicals using pyruvate as a precursor.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Tatiana Karaseva, Dmitry Fedorov, Sophia Baklagina, Olga Ponamoreva, Sergey Alferov, Galina Ekimova, Azat Abdullatypov, Liubov Trubitsina, Ildar Mustakhimov
Summary: This study reported the expression, purification, and properties of recombinant PQQ-MDH, as well as its application in methanol biosensors. The recombinant enzyme showed high enzymatic activity and stability, with a pH optimum of about 9-10. It was activated by ammonium ions and had a high affinity for methanol. The biosensor based on the recombinant enzyme exhibited competitive performance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Hoa Thi Quynh Le, Dung Hoang Anh Mai, Jeong-Geol Na, Eun Yeol Lee
Summary: In this study, a host strain for violacein production from non-sugar feedstocks, Methylorubrum extorquens AM1, was engineered using metabolic engineering. The results showed that by optimizing expression vectors and inducer concentrations, violacein production using methanol as the sole substrate was achieved. Random mutagenesis and site-directed mutagenesis further improved the production, and co-utilization of methanol and acetate significantly enhanced violacein production. This research provides a platform for economically efficient large-scale fermentation system for violacein production.
METABOLIC ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Arslan Sarwar, Eun Yeol Lee
Summary: Methanol is gaining attention as a potential carbon substrate for the production of fuels and chemicals due to its high reduction degree, abundance, and low price. Both native and synthetic methylotrophic microorganisms have been investigated for the production of biofuels and chemicals. However, the complex metabolic pathways, limited availability of genetic tools, and toxicity issues still pose challenges for achieving high-level production for commercial purposes. This article reviews the production of biofuels and chemicals from methanol by native and synthetic methylotrophs and discusses ways to improve their efficiency.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Review
Biochemical Research Methods
Patrick A. Sanford, Benjamin M. Woolston
Summary: Recent metabolic engineering efforts to import C1 catabolic pathways into non-methylotrophic bacteria have led to synthetic strains growing on methanol, but their growth rates and product yields are still inferior to native methylotrophs. Moreover, the development of genetic engineering tools has made native C1 utilizers more tractable, raising the question of whether it is better to use an engineered strain or a native host for the microbial assimilation of C1 substrates.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Evolutionary Biology
Jean-Baptiste Leducq, David Sneddon, Malia Santos, Domitille Condrain-Morel, Genevieve Bourret, N. Cecilia Martinez-Gomez, Jessica A. Lee, James A. Foster, Sergey Stolyar, B. Jesse Shapiro, Steven W. Kembel, Jack M. Sullivan, Christopher J. Marx
Summary: A comprehensive study on the taxonomy and evolution of Methylobacterium revealed four distinct bacterial groups and highlighted the significance of phyllosphere-associated species. It was suggested that Methylobacterium ancestor transitioned from a free-living lifestyle to association with plant roots, followed by colonization of the phyllosphere.
GENOME BIOLOGY AND EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Jihee Yoon, Woojin Chang, Seung-Hwan Oh, Soo-Hyung Choi, Yung-Hun Yang, Min-Kyu Oh
Summary: By genetic engineering of M. extorquens and addition of short-chain fatty acids, a PHBV copolymer with a high proportion of 3HV can be synthesized using formate as the carbon source.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
L. -P. Zhu, S. -Z. Song, S. Yang
Summary: The construction of a synthetic sRNA system has enabled genetic manipulation of M. extorquens strains, leading to interference with target genes and reduced production of carotenoids.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Agronomy
Felix Moran, Ester Marco-Noales, Elena Landeras, Montserrat Rosello, Adela Abelleira, Ana J. Gonzalez, Maria M. Lopez
Summary: The cultivation of kiwifruit in the European Union has attracted great economic interest due to high consumer demand and good production potential and fruit quality. A study identified a new group of Pseudomonas syringae that affects kiwifruit plants, which is different from the previously described lineages, demonstrating the diversity found in P. syringae species affecting kiwifruit plants.
Article
Biotechnology & Applied Microbiology
Hiroya Yurimoto, Hiroyuki Iguchi, Do Thi Di Thien, Akio Tani, Yutaka Okumoto, Atsushi Ota, Takahiro Yamauchi, Takahiro Akashi, Yasuyoshi Sakai
Summary: The foliar spray of PPFMs has been shown to improve crop yield of rice cultivars, demonstrating its feasibility for food production in the methanol bioeconomy.
MICROBIAL BIOTECHNOLOGY
(2021)
