Article
Chemistry, Physical
Sergei V. Raik, Tatiana V. Mashel, Albert R. Muslimov, Olga S. Epifanovskaya, Mikhail A. Trofimov, Daria N. Poshina, Kirill V. Lepik, Yury A. Skorik
Summary: The study found that TMAB-CS has a high affinity for DNA and demonstrated high transfection efficiency in HEK293T cells. However, it was less effective in K562 cells compared to polyarginine, albeit still more effective overall.
Article
Pharmacology & Pharmacy
Simon Decker, Alexander Taschauer, Emanuela Geppl, Viktoria Pirhofer, Michael Schauer, Stephan Poschl, Florian Kopp, Lars Richter, Gerhard F. Ecker, Haider Sami, Manfred Ogris
Summary: In this study, GE11 variants with improved binding were designed using a computational approach and their efficacy in transfection studies was validated.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Engineering, Biomedical
Haimei Hu, Han Wang, Shuanghong Liang, Xiaoling Li, Dan Wang
Summary: Poly(amido-amine) (PAMAM) modified by hyaluronic acid (HA) showed reduced cytotoxicity and smaller particle size, with improved DNA delivering capacity in cancer cells line. The transfection efficiency of the modified polymers approached that of unmodified polyplexes, making them potential candidates for targeted gene delivery.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2021)
Article
Pharmacology & Pharmacy
Yue Li, Ting Yu, Long-zhe Han, Li-li Jin, Yong Jin, Ji-shan Quan
Summary: In this study, a novel liposome-PEI complex was used to improve the efficiency of gene delivery, which showed high transfection efficiency in various cancer cell lines without causing systemic hematological toxicity. Compared to traditional approaches, the liposome-PEI complex mediated the entry of therapeutic genes into the body without damage to normal organs and enhanced tumor tissue apoptosis.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Plant Sciences
Ganeshan Sivanandhan, Solhee Bae, Chaemin Sung, Su-Ryun Choi, Geung-Joo Lee, Yong-Pyo Lim
Summary: Chinese cabbage is a significant dietary source of phytochemicals, but breeding elite cultivars is challenging due to a long cycle and complex genome. A protoplast transfection method was established for cell-based breeding and functional studies.
Article
Nanoscience & Nanotechnology
Liefeng Hu, Shuting Li, Yonggang Lv
Summary: In this study, a pH-sensitive linear poly(ortho ester) polycation was successfully prepared and used for gene transfection in neuroma cells. The polymer showed good biocompatibility and highly specified transfection efficiency on human neuroblastoma cells, with serum tolerance and deeper transfection depth. Compared with the control group, this polymer demonstrated great potential in neuroma therapy.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Junjie Pan, Chi-ling Chiang, Xinyu Wang, Paul Bertani, Yifan Ma, Junao Cheng, Vishank Talesara, Ly James Lee, Wu Lu
Summary: Nanochannel electroporation (NEP) is a new technology for cell transfection that offers improved gene delivery and cell viability compared to conventional bulk electroporation (BEP). NEP uses an asymmetric electric field to induce asymmetric membrane poration of cells laid on a porous substrate. This study systematically investigates the transfection window of NEP for different sized molecules and shows that small molecules can be delivered with minimal impact on the non-transfection membrane, while larger molecules require higher voltages but can result in lower cell viability. Numerical analysis of transmembrane potential allows for prediction of membrane damage and cargo delivery in NEP, providing a comprehensive understanding of the process.
Article
Engineering, Biomedical
Jeil Lee, Yong-Eun Kwon, Younjin Kim, Joon Sig Choi
Summary: PAMAM dendrimer, a widely studied polymer in biomedical research, shows potential as an alternative to PEI in gene delivery when conjugated with NLS sequences. This study suggests that PAMAM generation 2 with NLS sequences can reduce cytotoxicity while maintaining high transfection efficiency.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2021)
Article
Biochemistry & Molecular Biology
Richard Nii Lante Lamptey, Avinash Gothwal, Riddhi Trivedi, Sanjay Arora, Jagdish Singh
Summary: Multifunctional fatty acid grafted polymeric micelles have shown promise as an effective method for drug and gene delivery to the brain. Intranasal administration has been identified as an alternative approach to bypass the blood-brain barrier. In this research, polymeric micelles were prepared and characterized for the delivery of pVGF to the brain. The results showed that the modified chitosan polymer with mannose, Tat peptide, and oleic acid led to significantly higher expression of pVGF in primary astrocytes and neurons compared to intravenous administration. Intranasal administration also resulted in significantly higher pVGF expression compared to intravenous administration. These findings suggest that multifunctional polymeric micelles, especially those tagged with mannose and Tat ligands, are effective platforms for delivering pVGF to the brain.
Review
Biotechnology & Applied Microbiology
Kai Ma, Chun-Liu Mi, Xiang-Xiang Cao, Tian-Yun Wang
Summary: Gene delivery systems are crucial for gene therapy and recombinant protein production, with non-viral vector gene delivery reagents offering advantages such as large packaging load capacity and low immunogenicity. Liposomes and non-liposome cationic polymers are commonly used as gene delivery reagents, with different head structures including quaternary ammonium salt, amine, amino acid, polypeptide, guanidine salt, and a heterocyclic ring.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
A. Ramos-Valle, L. Marin-Caba, L. Garcia Hevia, M. A. Correa-Duarte, M. L. Fanarraga
Summary: Repairing genetic defects using exogenous DNA is a major challenge, and the design of effective vectors for encapsulating and delivering DNA to specific cells is crucial. The silica-based particles developed in this study provide stable encapsulation and protection of DNA, even under various stress conditions. The versatility of the design allows for the sequential expression of multiple genes, mimicking viral gene expression cascades, and the method is scalable, reproducible, and cost-effective, making it valuable for the personalized therapeutics industry.
MATERIALS TODAY ADVANCES
(2023)
Article
Nanoscience & Nanotechnology
Won Kyu Lee, Joonbum Lee, Jae-Seung Lee
Summary: In this study, highly stable DNA-poly-L-lysine (PLL) polyplexes were synthesized and used as cores to synthesize uniform gold nanoshells for photothermal therapy. By introducing gold nanoparticles (AuNPs) to the polyplex surface, the chemical and physical stability of the polyplexes were significantly enhanced, while maintaining their enzymatic biodegradability. The resulting poly@AuNSs exhibit excellent photothermal properties, high photothermal stability, and outstanding targeting ability towards cancer cells.
ACS APPLIED NANO MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Erica Quagliarini, Junbiao Wang, Serena Renzi, Lishan Cui, Luca Digiacomo, Gianmarco Ferri, Luca Pesce, Valentina De Lorenzi, Giulia Matteoli, Heinz Amenitsch, Laura Masuelli, Roberto Bei, Daniela Pozzi, Augusto Amici, Francesco Cardarelli, Cristina Marchini, Giulio Caracciolo
Summary: Lipid nanoparticles (LNPs) have shown promise as effective gene delivery vehicles, with a specific formulation (LNP15) demonstrating successful transfection and potential application in cancer vaccines.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Medicinal
Shuang Wu, Meiyan Liu, Xiang Hu, Chengxi He, Chunyan Zhao, Shuanglin Xiang, Youlin Zeng
Summary: The chemical and physical structure of cationic liposomes significantly affect their gene transfection efficiency; the design of novel cationic liposomes is guided by the structure-function relationship investigation; the study shows that different lipid structures play a crucial role in gene transfection efficiency.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Pharmacology & Pharmacy
Jose Antonio Lebron, Pilar Lopez-Cornejo, Elena Garcia-Dionisio, Pablo Huertas, Margarita Garcia-Calderon, Maria Luisa Moya, Francisco Jose Ostos, Manuel Lopez-Lopez
Summary: The tendency of surfactants to self-aggregate plays a key role in determining their efficiency for compacting nucleic acids, with 12NBr showing the most effectiveness as a DNA compacting agent. Lipoplexes of DOPE/12NBr/pEGFP-C1 were prepared and characterized, with investigations into their cytotoxicity and transfection efficiency.
Article
Materials Science, Biomaterials
Rajan Swami, Yogesh Kumar, Dasharath Chaudhari, Sameer S. Katiyar, Kaushik Kuche, Parmeshwar B. Katare, Sanjay K. Banerjee, Sanyog Jain
Summary: Co-delivering DTX and SIRT1 shRNA in a single tumor-specific nano-platform can improve the therapeutic potential of current therapy. Through systematic in vitro and in vivo examinations, DTX-lipoplex showed significantly higher DTX titre within tumor cells, reducing tumor burden by approximately 78% compared to conventional lipid transfection agents.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Chemistry, Analytical
Shrishty Bakshi, Samriddhi Mehta, Tushar Kumeria, Muhammad J. A. Shiddiky, Amirali Popat, Sangeeta Choudhury, Sudeep Bose, Ranu Nayak
Summary: This study presents a rapid fabrication and proof-of-concept demonstration of an electrochemical immunosensor based on hyper-branched gold nanostructures for protein biomarker detection. By optimizing the process, efficient immobilization of proteins was achieved with a quick assay timing of 4 hours. The sensor showed a linear working range, low limit-of-detection, high selectivity in detecting target proteins, and good stability and reusability.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biotechnology & Applied Microbiology
Ranjot Kaur, Sarah R. Dennison, Andrea J. Burrow, Shivaprakash M. Rudramurthy, Rajan Swami, Varun Gorki, O. P. Katare, Anupama Kaushik, Bhupinder Singh, Kamalinder K. Singh
Summary: The use of biomimetic surface-active lipid-polymer hybrid (LPH) nanoparticles of voriconazole has shown promising results in enhancing drug deposition and retention in the lungs, providing effective treatment for pulmonary aspergillosis infection.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Materials Science, Biomaterials
Aun Raza, Fekade Bruck Sime, Peter J. Cabot, Jason A. Roberts, James R. Falconer, Tushar Kumeria, Amirali Popat
Summary: Meropenem is currently only available in injectable form due to its poor stability, but a novel approach using mesoporous silica nanoparticles and coated with Eudragit polymer can enable specific delivery in the small intestine, improve cellular uptake efficiency, and retain antibacterial activity comparable to free Meropenem in solution.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Review
Pharmacology & Pharmacy
Ubaid Tariq, Shravan K. Uppulapu, Sanjay K. Banerjee
Summary: Glycogen synthase kinase 3 (GSK-3) is a widely expressed serine/threonine kinase that plays a crucial role in regulating various cellular processes, especially in cardiac biology. While its inhibition can promote cardiomyocyte proliferation and cardiac regeneration, it may also lead to hypertrophic myopathy. Therefore, GSK-3 inhibitors are considered a double-edged sword due to their beneficial and off-target effects in heart disease treatment.
CURRENT DRUG TARGETS
(2021)
Article
Pharmacology & Pharmacy
Aun Raza, Jared A. Miles, Fekade Bruck Sime, Benjamin P. Ross, Jason A. Roberts, Amirali Popat, Tushar Kumeria, James R. Falconer
Summary: A novel multi-material delivery system based on gamma-CD and PLGA was developed to enhance the solubility and stability of MER, while maintaining its antibacterial activity.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
John Ndayishimiye, Amirali Popat, Tushar Kumeria, Mark A. T. Blaskovich, James Robert Falconer
Summary: This study reports on efforts to improve the aqueous dissolution of Benznidazole (BZ) via processing and gamma-cyclodextrin complexation. The preparation methodology had substantial effects on the solid-state particle size/morphology characteristics and aqueous dissolution behavior of BZ, both alone or in complexes with gamma-CD, with potential to develop improved formulations.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Materials Science, Biomaterials
Vanessa Jane Banun, Prarthana Rewatkar, Zanib Chaudhary, Zhi Qu, Taskeen Janjua, Anuja Patil, Yuao Wu, Hang T. Ta, Nidhi Bansal, Jared A. Miles, Benjamin P. Ross, Tushar Kumeria, Amirali Popat
Summary: Coenzyme-Q10 (CoQ10) is encapsulated into milk derived protein nanoparticles to enhance its solubility, stability, and bioavailability. These nanoparticles significantly improve the solubility and permeability of CoQ10 by up to 300-fold compared to pure CoQ10. The study also demonstrates that the nanoparticles have a sustained release profile and enhanced antioxidant properties, making them a promising approach for improving oral delivery of poorly soluble drugs and nutraceuticals.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Biomedical
Aun Raza, Seyed Ebrahim Alavi, Fekade Bruck Sime, Felicity Y. Han, Jason A. Roberts, Amirali Popat, James R. Falconer, Tushar Kumeria
Summary: This study demonstrates a multi-functional nano-in-micro hierarchical microsphere system for controlling intestinal efflux pumps affecting the oral bioavailability of therapeutic drugs. The innovative materials combination allows for high loading of hydrophilic drugs while protecting them and reducing efflux with Eudragit (R) polymers. The prepared formulations significantly improve drug bioavailability and retain antibacterial activity against common pathogens.
ACTA BIOMATERIALIA
(2021)
Article
Instruments & Instrumentation
Weizhi Xu, Zonglan Zhao, James Falconer, Andrew K. Whittaker, Amirali Popat, Maree T. Smith, Tushar Kumeria, Felicity Y. Han
Summary: The study found that using a large scCO(2) vessel increased the encapsulation efficiency of ketamine, while pre-loading ketamine into pSi also slightly improved the drug incorporation level. Additionally, the use of a large scCO(2) vessel reduced burst release and extended the release period for PLGA microparticles containing ketamine.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Food Science & Technology
Harsha Pal, Ramandeep Kaur, Pankaj Kumar, Manju Nehra, Kritika Rawat, Nishant Grover, Ozlem Tokusoglu, Loveleen Kaur Sarao, Sandeep Kaur, Tanu Malik, Ajay Singh, Rajan Swami
Summary: The study found that adding star fruit juice can increase the antioxidant activity and total phenolic content of beer, while improving sensory characteristics, especially beer fortified with 10% star fruit receiving the highest overall acceptability;Star fruit can serve as a valuable fortification compound for producing beer with high nutritional value.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Article
Materials Science, Multidisciplinary
Hadi Ebrahimi-Darkhaneh, Zeshaan Shamsi, Martin G. R. Banda, Manuel Quevedo-Lopez, Luigi Colombo, Sanjay K. Banerjee
Summary: This article proposes a novel design approach for lateral Schottky barrier diodes using a wide bandgap oxide semiconductor on silicon. The fabricated device's structural and electrical properties are reported and compared. Due to the low-temperature process, simplicity of fabrication steps, and high figure-of-merit, the proposed diode shows great potential for power electronics applications.
PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE
(2022)
Article
Cell Biology
Hina L. Nizami, Parmeshwar B. Katare, Pankaj Prabhakar, Ramu Adela, Soumalya Sarkar, Sudheer Arava, Praloy Chakraborty, Subir K. Maulik, Sanjay K. Banerjee
Summary: The study demonstrates that paricalcitol can reverse cardiac dysfunction in rats with metabolic syndrome by enhancing mitochondrial fusion.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Pharmacology & Pharmacy
Sushil Yadaorao Raut, Kengyen Fu, Huang Taichun, Avinash Gahane, Dasharath Chaudhari, Varun Kushwah, Renuka Suresh Managuli, Aswathi R. Hegde, Sanyog Jain, Guruprasad Kalthur, Manjunath Bandu Joshi, Hsin-I. Chang, Niann-Tzyy Dai, Srinivas Mutalik
Summary: To address the issue of female infertility, researchers have developed an oral formulation of follicle stimulating hormone (FSH) using nanostructured lipid carriers (NLCs) and liposomes as carriers to improve the oral bioavailability and efficacy of the drug. Through a series of experiments and studies, they demonstrated the stability and effectiveness of the developed formulations in vivo.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Navya Malladi, Md Jahangir Alam, Subir K. Maulik, Sanjay K. Banerjee
Summary: Platelets play a crucial role in thrombosis and the progression of many diseases. In non-alcoholic fatty liver disease (NAFLD), platelets are highly activated and contribute to disease progression by promoting a pro-thrombotic and pro-inflammatory state. Various platelet parameters have been found to be associated with NAFLD. Furthermore, platelets are involved in the development of cardiovascular complications in NAFLD. Modulating platelet function using medications like aspirin, ticlopidine, and cilostazol has shown potential in controlling NAFLD progression. Future research should focus on antiplatelet therapy to control platelet activation and reduce cardiovascular risk in NAFLD.
PROSTAGLANDINS & OTHER LIPID MEDIATORS
(2023)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)