Article
Chemistry, Multidisciplinary
Seyong Chung, Si Yeong Kim, Kyubae Lee, Sewoom Baek, Hyun-Su Ha, Dae-Hyun Kim, Suji Park, Chan Hee Lee, Hye-Seon Kim, Young Min Shin, Seung Eun Yu, Hak-Joon Sung
Summary: This study explores the liposomal targeting of splenic CD11b(+) cells and the induction of in situ reprogramming through the use of hypoxic-mimetic agent-loaded liposomes. The reprogrammed cells show enhanced vasculogenic potential, leading to accelerated regeneration in inflamed tissues. Advanced tools such as intravital multiphoton microscopy, F-19 2D/3D MRI, and microchannel hydrogel chips are used to validate the strategy.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Cell & Tissue Engineering
Ning Zhou, Lei Wang, Ping Fu, Zihao Cui, Yuhang Ge, Feiyu Jiang, Jing Liu, Chao Ren, Zuo Luan, Hongbin Fan, Ruiqin Yao
Summary: EPCs enhance oligovascular remodeling and decrease OPC apoptosis, while EC-pEPCs are more effective in promoting oligovascular remodeling and myelination through the CXCL12-CXCR4 axis in the neonatal rat WMI model. EPCs and CXCL12 protein levels significantly increase in WMI rats, and EC-pEPCs show notable improvements in vascular density, myelination, and cognitive outcomes compared to EPCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Lingchi Kong, Yan Wang, Haixing Wang, Qi Pan, Rongtai Zuo, Shanshan Bai, Xiaoting Zhang, Wayne Yukwai Lee, Qinglin Kang, Gang Li
Summary: The study showed that microgravity enhances the pro-angiogenic properties of EPCs' paracrine signals through the HIF-1α/eNOS/NO axis, and the administration of MG-CM promotes bone fracture repair.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Immunology
Qun Dong, Jing Tian, Weiwei Zheng, Qiuxiang Fan, Xiaoyong Wu, Yanli Tang, Tao Liu, Hui Yin
Summary: Peripheral vascular disease often results in vascular injury and inflammation. This study investigates the role of IL-33 in hindlimb ischemic injury and its potential mechanisms. The findings suggest that IL-33 promotes blood flow restoration and enhances repair in ischemic hindlimb by modulating the proangiogenic function of endothelial cells through the Akt/eNOS pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Paulina Podkalicka, Olga Mucha, Katarzyna Kazirod, Iwona Bronisz-Budzynska, Sophie Ostrowska-Paton, Mateusz Tomczyk, Kalina Andrysiak, Jacek Stepniewski, Jozef Dulak, Agnieszka Loboda
Summary: The study compared angiogenesis-related alterations in mdx mice and wild-type counterparts, revealing different dysregulation of angiogenic factors in mdx mice of different ages; older mdx mice exhibited more significant angiogenic dysfunction.
Article
Chemistry, Multidisciplinary
Yuen Ting Lam, Bob S. L. Lee, Juichien Hung, Praveesuda Michael, Miguel Santos, Richard P. Tan, Renjing Liu, Steven G. Wise
Summary: This study demonstrates the successful delivery of therapeutic miRNAs using plasma-polymerised nanoparticles (PPNs). It also shows that PPN delivery of a miR-503 inhibitor can enhance endothelial angiogenesis and promote blood-perfusion recovery in diabetic patients.
Article
Cell Biology
Miriam Di Mattia, Annunziata Mauro, Simona Delle Monache, Fanny Pulcini, Valentina Russo, Paolo Berardinelli, Maria Rita Citeroni, Maura Turriani, Alessia Peserico, Barbara Barboni
Summary: This study found that an appropriate dose of cobalt chloride can be used as a hypoxia-mimetic agent in amniotic epithelial stem cells (AECs) to enhance their anti-angiogenic properties. This provides a novel approach for stem cell-free therapy protocols.
Article
Biochemistry & Molecular Biology
Joon Seok Lee, Hyun Geun Cho, Jeong Yeop Ryu, Eun Jung Oh, Hyun Mi Kim, Suin Kwak, Seok-Jong Lee, Jongmin Lee, Sang Yub Lee, Seung Huh, Ji Yoon Kim, Ho Yun Chung
Summary: This study analyzed the effects of hypoxia on angiogenesis in arteriovenous malformation (AVM) patients and normal tissues. The results showed that AVM ECs exhibited higher angiogenesis capacity and expression of angiogenesis-related genes under hypoxic conditions. These findings provide a foundation for the development of AVM treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Clinical Neurology
Antia Custodia, Alberto Ouro, Joao Sargento-Freitas, Marta Aramburu-Nunez, Juan Manuel Pias-Peleteiro, Pablo Hervella, Anna Rosell, Lino Ferreira, Jose Castillo, Daniel Romaus-Sanjurjo, Tomas Sobrino
Summary: Ischemic stroke is a common cause of death and disability in developed countries. Current therapeutic options are limited, so there is a need for novel treatments. Endothelial progenitor cells (EPCs) have been shown to repair damaged vessels and generate new ones. EPCs appear to be a promising target for stroke treatment, promoting re-endothelialization, angiogenesis, and vasculogenesis.
FRONTIERS IN NEUROLOGY
(2022)
Article
Plant Sciences
Yonghui Dou, Yue Shu, Yaoyu Wang, Dan Jia, Zhengyun Han, Beiyin Shi, Jieying Chen, Jie Yang, Zhen Qin, Shuiqing Huang
Summary: Danggui Buxue Decoction (DBD), a classic prescription of traditional Chinese medicine, has been used for over 800 years to treat clinical anemia. It has the potential to improve angiogenesis and promote neuroprotection. Combining DBD with bone marrow-derived endothelial progenitor cells (EPCs) transplantation may be a novel strategy for treating focal cerebral ischemia (FCI). This study aimed to investigate the mechanistic effects of DBD in combination with EPCs transplantation on improving behavioral function and hyperlipidemia in FCI.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Cell Biology
Huang-Ming Chang, Kang-Yung Peng, Chieh-Kai Chan, Chiao-Yin Sun, Ying-Ying Chen, Han-Mei Chang, Chun-Lin Huang, Pei-Chun Liu, Peng-Ying Chen, Kuo-Chuan Wang, Wei-Jie Wang, Chen-Chi Wu, Yu-Feng Lin, Tai-Shuan Lai, Tao-Min Huang, Guang-Huar Young, Shuei-Liong Lin, Marlies Ostermann, Tzong-Shinn Chu, Jeff S. Chueh, Vin-Cent Wu
Summary: This study reveals the protective role of FGF23 in AKI, promoting renal regeneration, vascular repair, and attenuating damage. FGF23 interacts with the SDF-1/CXCR4 signaling, inhibiting EPC senescence and migration, while not affecting angiogenesis.
CELL DEATH & DISEASE
(2021)
Article
Engineering, Biomedical
Kevin T. Campbell, Matthew B. Curtis, Joshua M. Massey, Kajetan Wysoczynski, Dustin J. Hadley, Steve C. George, Eduardo A. Silva
Summary: Lymphatic dysfunction is associated with the progression of several vascular disorders, and there are limited strategies currently available to promote new lymphatic vasculature. This study isolated lymphatic endothelial progenitor cells from human umbilical cord blood and found that they have the potential to promote lymphatic sprouts, highlighting the therapeutic potential of these cells. Delivery of lymphatic EPCs using alginate hydrogels also showed promise for possible therapeutic applications.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Multidisciplinary
Cheng Peng, Li-jun Yang, Chuan Zhang, Yu Jiang, Liu-wen-xin Shang, Jia-bei He, Zhen-wei Zhou, Xia Tao, Lu Tie, Alex F. Chen, He-hui Xie
Summary: Low-dose nifedipine has been found to enhance the angiogenic potential of endothelial progenitor cells (EPCs) and protect against cerebral ischemic injury in diabetic mice. The treatment with nifedipine significantly increased the number of circulating EPCs, improved EPC function, and promoted angiogenesis. It also reduced the levels of inhibitory factors in EPCs. This study suggests that long-term treatment with low-dose nifedipine can be a safe and cost-effective strategy to prevent ischemic diseases in diabetes.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Cell Biology
Kenon Chua, Fui Ping Lim, Victor Kwan Min Lee, Toan Thang Phan, Bee Choo Tai, Yih Kai Tan
Summary: In this study, human Cord Lining Mesenchymal Stem Cells (CL-MSCs) were used in a rabbit hindlimb ischemia model to stimulate neovascularization. The results showed that CL-MSCs had a positive effect on angiogenesis, with more CD31 positive cells and capillaries found in the treated muscles. Additionally, there was a non-statistically significant increase in blood perfusion in the treatment group, and CL-MSCs showed no toxicity or significant local immune reaction.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yang Yu, Xuechun Li, Yimei Li, Renyue Wei, Hai Li, Zhonghua Liu, Yu Zhang
Summary: This study establishes a stable iPSC-EC induction system and investigates the regulatory mechanism of endothelial differentiation. The derived piPSC-ECs form microvessel-like structures in vitro and promote blood vessel formation under pathological conditions. The activation of TGF-beta-Smad2/3 signaling promotes mesoderm formation and prevents endothelial differentiation in this model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Parisa Eslami, Martin Albino, Francesca Scavone, Federica Chiellini, Andrea Morelli, Giovanni Baldi, Laura Cappiello, Saer Doumett, Giada Lorenzi, Costanza Ravagli, Andrea Caneschi, Anna Laurenzana, Claudio Sangregorio
Summary: In this study, drug-loaded smart magnetic nanocarriers were successfully created using superparamagnetic iron oxide nanoparticles encapsulated in a dual pH- and temperature-responsive copolymer. These nanocarriers exhibited reversible hydration/dehydration transition at acidic conditions and/or temperatures above physiological body temperature, which could be triggered by magnetic hyperthermia. The effectiveness of the system was demonstrated by high encapsulation efficiency of the drug at neutral pH and the release of the drug under hyperthermia conditions. This study suggests that this multi-stimuli-sensitive nanoplatform has great potential for remote-controlled drug release combined with magnetic hyperthermia for cancer treatment.
Article
Biochemistry & Molecular Biology
Brunella Grassiri, Patrick Knoll, Angela Fabiano, Anna Maria Piras, Ylenia Zambito, Andreas Bernkop-Schnuerch
Summary: The goal of this study was to design and evaluate a thiolated cyclodextrin for ocular drug delivery, which provides high drug solubilizing and mucoadhesive properties. The results showed that thiolated cyclodextrin significantly enhanced the precorneal residence time and bioavailability of dexamethasone eye drops, making it suitable for ocular formulations of poorly soluble drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Francesca Felice, Maria Michela Cesare, Luca Fredianelli, Marinella De Leo, Veronica Conti, Alessandra Braca, Rossella Di Stefano
Summary: Tomatoes and their derivates contain natural biologically active components that have a protective effect on muscle aging. This study found that tomato peel extracts grown in drought stress conditions significantly reduced muscle atrophy induced by dexamethasone and decreased the expression of the muscle atrophy-related protein FOXO1, thereby providing protection against sarcopenia in an in vitro model.
Article
Pharmacology & Pharmacy
Angela Fabiano, Marinella De Leo, Luca Cerri, Anna Maria Piras, Alessandra Braca, Ylenia Zambito
Summary: This study successfully prepared self-assembling saffron extract nanoparticles with the assistance of chitosan, which protected crocin from degradation in simulated tear fluid and increased the residence time in the eyes.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Virology
Arianna Baronti, Francesco Gentile, Alice Chiara Manetti, Andrea Scatena, Silvia Pellegrini, Angela Pucci, Maria Franzini, Vincenzo Castiglione, Aniello Maiese, Alberto Giannoni, Mauro Pistello, Michele Emdin, Giovanni Donato Aquaro, Marco Di Paolo
Summary: This study presents five cases of sudden death secondary to myocardial infarction (MI) closely related to COVID-19 vaccination. Pathological analysis and genetic testing revealed that the patients carried pro-thrombotic mutations, suggesting a possible link between COVID-19 vaccination, pro-thrombotic genotypes, and acute cardiovascular events that requires further investigation.
Article
Polymer Science
Brunella Grassiri, Andrea Cesari, Federica Balzano, Chiara Migone, Gergely Kali, Andreas Bernkop-Schnurch, Gloria Uccello-Barretta, Ylenia Zambito, Anna Maria Piras
Summary: This study successfully synthesized a freely soluble thiolated 2-methyl-beta-cyclodextrin (M beta CD-SH) through a green microwave-assisted process, and characterized its inclusion complex properties with dexamethasone (Dex) and mucoadhesive characteristics. The results showed that the cyclodextrin was able to host the drug and increase the complex viscosity of mucin.
Article
Environmental Sciences
Giovanni Donato Aquaro, Benedetta Guidi, Michele Emdin, Angela Pucci, Enrica Chiti, Alessandro Santurro, Matteo Scopetti, Federico Biondi, Aniello Maiese, Emanuela Turillazzi, Giovanni Camastra, Lorenzo Faggioni, Dania Cioni, Vittorio Fineschi, Emanuele Neri, Marco Di Paolo
Summary: This study evaluated the accuracy of post-mortem cardiac magnetic resonance (PMCMR) for diagnosing the cardiac causes of sudden death. The results showed that PMCMR had high sensitivity and specificity in identifying the cardiac cause of sudden death, and could be considered as a valuable tool for forensic diagnosis.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Brunella Grassiri, Andrea Mezzetta, Giuseppantionio Maisetta, Chiara Migone, Angela Fabiano, Semih Esin, Lorenzo Guazzelli, Ylenia Zambito, Giovanna Batoni, Anna Maria Piras
Summary: The study evaluated the use of ionic liquids (ILs) as functional excipients for solubilizing and stabilizing diacerein (DIA) in eye drop formulations and prolonging its ocular residence time. Two ILs based on natural zwitterionic compounds were investigated: L-carnitine C6 alkyl ester bromide (Carn6) and betaine C6 alkyl ester bromide (Bet6). The ILs demonstrated solubilizing, stabilizing, mucoadhesive, and antimicrobial properties, with potential for future use in ophthalmic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Veronica Musetti, Francesco Greco, Vincenzo Castiglione, Alberto Aimo, Cataldo Palmieri, Dario Genovesi, Assuero Giorgetti, Michele Emdin, Giuseppe Vergaro, Liam A. McDonnell, Angela Pucci
Summary: Cardiac amyloidosis (CA) has historically been considered rare, but advances in diagnostic tools have led to a reassessment of its epidemiology. Accurate typing of cardiac amyloidosis is crucial due to differences in treatment and prognosis. Tissue characterization remains the gold standard for diagnosis and typing, particularly in AL amyloidosis.
Article
Pharmacology & Pharmacy
Lucia Vizzoni, Chiara Migone, Brunella Grassiri, Ylenia Zambito, Baldassare Ferro, Paolo Roncucci, Filippo Mori, Alfonso Salvatore, Ester Ascione, Roberto Crea, Semih Esin, Giovanna Batoni, Anna Maria Piras
Summary: Acute respiratory distress syndrome (ARDS) is a severe complication of lung injuries, often caused by various infections. ARDS is associated with high mortality and lacks effective treatment. In this study, the efficacy of mesh nebulisation of plasminogen (PLG) for off-label administration as an ARDS treatment was investigated. Results showed that aerosolised PLG demonstrated optimal deposition, maintained enzymatic activity, and had immunomodulating effects.
Article
Biochemistry & Molecular Biology
Pratigyan Dash, Sasmita Samal, Gyanendra Prasad Panda, Anna Maria Piras, Mamoni Dash
Summary: This study reports the preparation and evaluation of a nanoformulation that can reduce the side effects of Zol drug. The nanoformulation shows cytotoxic effects on bone cancer cells and protective effects on normal bone cells. The results suggest that Zol nanoformulation can serve as a good platform for a sustained release system with minimal side effects on normal bone cells.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Gianni Pecorini, Simona Braccini, Gianluca Parrini, Federica Chiellini, Dario Puppi
Summary: Polyhydroxyalkanoates are biopolyesters that can be used as unique scaffolding polymer candidates for tissue engineering due to their biocompatibility, biodegradability, environmental sustainability, processing versatility, and mechanical properties. This study explored the fabrication of tissue engineering scaffolds using a solution-extrusion additive manufacturing technique, called Computer-Aided Wet-Spinning (CAWS), by blending poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(D,L-lactide-co-glycolide) (PLGA). The results showed that the blend composition influenced the morphological, physicochemical, and biological properties of the scaffolds, with increasing PLGA content leading to larger pore size, enhanced wettability, and improved thermal stability. In vitro experiments demonstrated the suitability of the scaffolds for cell colonization and differentiation towards an osteoblastic phenotype, particularly with scaffolds richer in PLGA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Inorganic & Nuclear
Silvia Schoch, Simona Braccini, Lorenzo Biancalana, Alessandro Pratesi, Tiziana Funaioli, Stefano Zacchini, Guido Pampaloni, Federica Chiellini, Fabio Marchetti
Summary: In this study, a series of cationic triiron complexes resulting from the conjugation of ferrocenyl skeleton with a diiron bis-cyclopentadienyl core were synthesized and their cytotoxicity on cancer cell lines and non-tumoral cells was evaluated. The results showed that most of the cationic complexes exhibited low toxicity in cancer cells and non-tumoral cells.
INORGANIC CHEMISTRY FRONTIERS
(2022)
Article
Pathology
Angela Pucci, Alessandra Bacca, Ivana Barravecchia, Iose Di Stefano, Beatrice Belgio, Daniele Lorenzini, Liborio Torregrossa, Serena Chiacchio, Caterina Congregati, Gabriele Materazzi, Mauro Ferrari, Debora Angeloni, Giampaolo Bernini, Fulvio Basolo
Summary: This study investigated the correlations between histological findings, sclerosis, immunoreactivity for vesicular catecholamine transporters, and patients' genotype in paragangliomas and pheochromocytomas. The study found that sclerosis and VMAT1 expression were higher in paragangliomas and were associated with germ-line mutations.
HEAD & NECK PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Simona Braccini, Chiara Tacchini, Federica Chiellini, Dario Puppi
Summary: This article provides an overview of the latest research on in vitro tumor models, with a focus on three-dimensional OC cell culture using hydrogel-based scaffolds. These models can better replicate the tumor microenvironment observed in vivo, making them valuable tools for cancer research and drug screening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
