Review
Chemistry, Multidisciplinary
Qiuxin Zhang, Weiyi Tan, Bing Xu
Summary: Enzymatic noncovalent synthesis (ENS) is a mechanism that utilizes enzymatic reactions to produce spatially organized higher-order supramolecular assemblies, which can modulate cellular processes. This Review highlights the recent progress in the application of ENS for targeted subcellular organelles, showcasing its potential in understanding diseases, controlling cell behaviors, and developing new therapeutics.
Article
Pharmacology & Pharmacy
Haifeng Hu, Peng Li, Jie Qiu, Meiji Zhao, Mingjie Kuang, Zhaoyan Zhang, Dachuan Wang
Summary: This study used two-photon laser confocal microscopy to analyze the uptake and subcellular distribution of red fluorescent graphene quantum dots (Red-GQDs) in HeLa cells and discussed their effects on cell metabolism. The results showed that Red-GQDs were well-absorbed by HeLa cells and distributed in lysosomes and mitochondria in a time-dependent manner. Additionally, Red-GQDs significantly affected cell functions, inducing autophagy at low concentrations and promoting cell death and exosome secretion at high concentrations.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Maria J. Sarmento, Alicia Llorente, Toni Petan, Denis Khnykin, Iuliana Popa, Matea Nikolac Perkovic, Marcela Konjevod, Morana Jaganjac
Summary: Lipids in cell membranes and subcellular compartments are important for cellular processes, and specific organelle lipidomes are characterized by lipid synthesis, metabolism, trafficking, and homeostasis. Identifying the lipid fingerprints of cellular organelles has been challenging due to the variety and complexity of lipids, and the variability in their abundance. This review summarizes the available information on the lipid composition of mammalian cell organelles and discusses the dynamics of subcellular lipidomes under physiological and pathological conditions, as well as the challenges in mapping organelle lipidomes.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Siyuan Qin, Yuxin Zhang, Yuan Tian, Fengguo Xu, Pei Zhang
Summary: Metabolomics, a technique profiling global small molecules in biological samples, has become crucial for disease diagnosis and mechanism research. Organelles in cells, where diverse metabolic activities happen orderly, play a significant role in various pathological conditions. Characterizing the metabolic pool in compartmentalized system is important for understanding the role of organelles in physiological functions and diseases.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Environmental Sciences
Yiteng Xia, Wen-Xiong Wang
Summary: Acute toxicity determination is crucial for ecological risk assessment. Traditional methods require large numbers of animals and take days to obtain results. A cell-based fluorescent assay was developed as an alternative, which allows visualization and quantification of subcellular responses, providing a faster and cheaper method for predicting the acute toxicity of sewage effluents.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Review
Chemistry, Multidisciplinary
Wenyao Zhen, Shangjie An, Shuqi Wang, Wenxue Hu, Yujie Li, Xiue Jiang, Jinghong Li
Summary: Despite the significant progress in tumor therapies, limitations such as low penetration depth of laser, safety concerns of radiation, therapeutic resistance, and spatio-temporal constraints of specific equipment hinder their clinical applications. In addition, physiological barriers in the tumor microenvironment and the advantages of endogenous stimuli have driven the development of nanoplatforms and subcellular organelle-targeted strategies. These advancements may lead to improved precision medicine and clinical translation of multifunctional nanomedicines in the future.
ADVANCED MATERIALS
(2021)
Review
Medicine, Research & Experimental
Therese C. Kichuk, Cesar Carrasco-Lopez, Jose L. Avalos
Summary: Optogenetics utilizes light-sensitive proteins to manipulate cellular function and structure, offering novel ways to control subcellular organization. By targeting specific organelles, researchers can precisely manipulate cellular functions like nuclear translocation and plasma membrane morphology, while also exploring organelle dynamics and function. This technology has the potential to revolutionize biological research and bioengineering applications by providing spatiotemporal control over cellular processes.
WIRES MECHANISMS OF DISEASE
(2021)
Article
Chemistry, Analytical
Yan Zhang, Keyin Li, Yaoyao Zhao, Weihua Shi, Hrishikesh Iyer, Sungho Kim, Christopher Brenden, Jonathan V. Sweedler, Yurii Vlasov
Summary: We demonstrate a silicon-based integrated microfluidic platform for multiplexed analysis of neurochemicals in picoliter droplets via nanoelectrospray ionization (nESI)-mass spectrometry (MS). The developed system allows effective detection of multiple neurochemicals in low picoliter volumes, showing promising applications in monitoring a range of brain chemicals.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Adriana Maria Diaz-Anaya, Louise Gerard, Martine Albert, Jean-Francois Gaussin, Marielle Boonen, Jean-Pierre Gillet
Summary: ABCB5 beta is a marker protein associated with melanoma stem cells and skin progenitor cells. The study reveals that ABCB5 beta is predominantly localized to the endoplasmic reticulum and is degraded by the proteasome system. Treatment with SAHA, a molecule promoting chaperone-assisted folding, increases the expression of ABCB5 beta.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Chunfei Wang, Hengyi Fu, Jingyun Tan, Xuanjun Zhang
Summary: A reversible pH-responsive fluorescent probe, BP, was designed and synthesized based on protonation and deprotonation-induced oxazolidine ring open and close. The probe exhibited strong fluorescence response to pH, making it suitable for labeling intracellular pH-dependent organelles. In living cells, BP showed good colocalization with mitochondria and lysosomes, attributed to the pH-induced structure tautomerism effect.
Review
Biotechnology & Applied Microbiology
Lu Yao, Xiuming Wu, Xun Jiang, Muhammad Shan, Zhuoxiang Zhang, Yiting Li, Aiguo Yang, Yu Li, Changqing Yang
Summary: Plant natural products (PNPs) are specialized metabolites synthesized in plant cells by enzymes. Understanding the subcellular compartmentalization of their biosynthesis is important for sustainable production. This review describes the role of subcellular compartments in the biosynthesis of major PNPs and discusses challenges and strategies in the field of plant synthetic biology and subcellular engineering.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Nanoscience & Nanotechnology
Jin Liu, Le Yang, Caoye Xue, Ge Huang, Shiya Chen, Jing Zheng, Ronghua Yang
Summary: Monitoring hypoxia-related changes in subcellular organelles has been facilitated by a new nanodevice incorporating light-responsive DNA probes into a hypoxia-responsive metal-organic framework, enabling spatiotemporally controlled imaging of biomolecules. This approach shows promise for monitoring dynamic changes in subcellular organelles under hypoxic conditions.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Applied
Lulu Li, Dan He, Xiaodong Chen, Sanxiu Yang, Yunfei Yang, Aihua Gong, Kun Wang, Jing Qian, Lingliang Long
Summary: Visualizing minor pH fluctuations in subcellular organelles is important for studying physiological events in living organisms. In this study, a family of coumarin-indolenium based fluorescent pH probes were developed to visualize these minor pH fluctuations. The probes can be tuned by multiple approaches to accurately detect pH changes in different subcellular organelles. They have been successfully used to track pH fluctuation in mitochondria, lysosome, endoplasmic reticulum, as well as in zebrafish under high levels of exogenous CO2.
Article
Chemistry, Multidisciplinary
Gholamreza Mohammadi Khunsaraki, Hanieh Niroomand Oscuii, Arkady Voloshin
Summary: The mechanical behavior of living cells can be described using a tensegrity model. A finite element model was used to assess the mechanical contributions of subcellular organelles, showing that the cytoskeleton and cytoplasm are the main components of the cell's mechanical response, with the nucleoskeleton being stiffer.
APPLIED SCIENCES-BASEL
(2021)
Article
Environmental Sciences
Qian Liu, Xuexi Tang, Xin Zhang, Xin Tong, Zijie Sun, Xinxin Zhang
Summary: This study investigated the toxic effects of Triphenyl phosphate (TPhP) on marine diatoms. The results showed that TPhP can inhibit the population growth, alter ultrastructure, affect physiological activities, and change gene expression of the diatoms. Furthermore, TPhP was found to damage the function of mitochondria and chloroplasts.
ENVIRONMENTAL POLLUTION
(2022)
Article
Plant Sciences
Weiqiang Li, Yaru Sun, Kun Li, Hongtao Tian, Jiangtao Jia, Hongyu Zhang, Yaping Wang, Hong Wang, Baodi Bi, Jinggong Guo, Lam-Son Phan Tran, Yuchen Miao
Summary: Sinapate esters play important roles in stomatal closure in Arabidopsis thaliana, promoting UV-B induced malate accumulation and stomatal closure, while sinapic acid inhibits the ROS-NO pathway associated with UV-B induced stomatal closure.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Mostafa Abdelrahman, Mohammad Golam Mostofa, Cuong Duy Tran, Magdi El-Sayed, Weiqiang Li, Saad Sulieman, Maho Tanaka, Motoaki Seki, Lam-Son Phan Tran
Summary: This study investigated the potential role of the karrikin receptor KARRIKIN INSENSITIVE2 (KAI2) in the response of Arabidopsis seedlings to high-temperature stress. The results showed that kai2 mutants exhibited hypersensitivity to high-temperature stress and had distinct transcriptome profiles compared to wild-type plants.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Environmental Sciences
Arnab Majumdar, Munish Kumar Upadhyay, Biswajit Giri, Jayant Karwadiya, Sutapa Bose, Manoj Kumar Jaiswal
Summary: This study found that using rice straw to produce rice biochar and doping it with iron oxide nanoparticles can reduce arsenic contamination in paddy soils and improve the bioavailability of soil macro-nutrients. The 1% rice biochar and iron oxide conjugate showed the best performance, enhancing stress tolerance and improving internal vascular structure in rice plants. Therefore, iron oxide-doped rice biochar can be used as a sustainable amendment to improve soil-crop quality.
Review
Plant Sciences
Polavarapu B. Kavi Kishor, Showkat Ahmad Ganie, Shabir H. Wani, Rajasheker Guddimalli, Appa Rao Karumanchi, Sujatha Edupuganti, Jalaja Naravula, Vinay Kumar, Rathnagiri Polavarapu, Prashanth Suravajhala, Suprasanna Penna
Summary: NF-Y is a ubiquitous family of transcription factors that regulate plant growth, development, and stress responses. They interact with various signaling pathways to modulate plant stress responses and play important roles in multiple aspects of plant physiology.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
SumanKalyan Sadhu, Phanikanth Jogam, Kranthikumar Gande, Vasudha Marapaka, Suprasanna Penna, Venkataiah Peddaboina
Summary: This study successfully obtained transgenic chickpea plants with different levels of resistance to Fusarium wilt disease by introducing Radish defensin genes. The results suggest that Radish defensins can be used to improve pathogen resistance in chickpea.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Plant Sciences
Aarti Gupta, Mamta Bhardwaj, Lam-Son Phan Tran
Summary: High-yield crop varieties are highly sought after due to the increasing human population. The regulation of yield traits involves complex interactions of molecular and genetic factors controlled by various quantitative trait loci (QTLs). Recent studies have identified and characterized yield-associated QTLs, highlighting the central role of phytohormones in controlling plant yield. Different groups of phytohormones often work together to determine yield attributes, and understanding this interaction can facilitate the improvement of crop yield through the manipulation of a single gene or QTL(s). In this review, we discuss important findings regarding the roles of auxin, brassinosteroid, and cytokinin, and how a single gene or QTL can regulate cross talk among multiple phytohormones to determine yield traits.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Nidhi Kandhol, Vijay Pratap Singh, Jason C. White, Lam-Son Phan Tran, Durgesh Kumar Tripathi
Summary: The global increase in nanotechnology applications in agriculture and food production has been unprecedented. This article critically evaluates the existing literature on the interactions between nanoparticles and phytohormones in plants. It also explores the developing strategy of nano-enabled precision delivery of phytohormones and identifies future research directions and knowledge gaps in this important aspect of nano-enabled agriculture.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Mohammad Golam Mostofa, Mostafa Abdelrahman, Md Mezanur Rahman, Cuong Duy Tran, Kien Huu Nguyen, Yasuko Watanabe, Misao Itouga, Weiqiang Li, Zhe Wang, Keiichi Mochida, Lam-Son Phan Tran
Summary: The KAI2 receptor plays an important role in plant tolerance to salt stress. Defects in KAI2 functions result in impaired seed germination and plant growth under salt stress, leading to ion imbalance, oxidative damage, and abnormal hormone signaling.
PLANT AND CELL PHYSIOLOGY
(2023)
Review
Plant Sciences
Krishnananda P. Ingle, P. Suprasanna, Gopal Wasudeo Narkhede, Antony Ceasar, Gholamreza Abdi, Aamir Raina, M. P. Moharil, Atul Singh
Summary: Biofortification of foxtail millet is an important approach to alleviate micronutrient malnutrition. Foxtail millet has adequate nutrients and can be genetically modified to enhance its nutritional value. By utilizing the genomic information of foxtail millet and combining various techniques, it can become a model system for biofortification.
PLANT GROWTH REGULATION
(2023)
Review
Engineering, Environmental
Sandip A. Ghuge, Ganesh Chandrakant Nikalje, Ulhas Sopanrao Kadam, Penna Suprasanna, Jong Chan Hong
Summary: The accumulation of heavy metals in the ecosystem is a major environmental concern, and insights into the mechanisms of metal accumulation and tolerance in plants are needed. Certain plant species are considered good for metal cleanup. Studying plant root architecture, transporters, and metabolic pathways can improve plant tolerance to heavy metal stress and provide sustainable plant-based solutions for mitigating heavy metal toxicity.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Plant Sciences
Kamlesh K. Meena, Utkarsh Bitla, Ajay M. Sorty, Satish Kumar, Shrvan Kumar, Goraksha C. Wakchaure, Dhananjaya P. Singh, Peter Stougaard, P. Suprasanna
Summary: Drought and salinity, as abiotic stresses, have become frontier areas in agricultural research due to their potential threats to global food security. Soil salinity has severely damaged the production of staple food crops worldwide. To address these constraints, strategies have been developed to increase stress tolerance and mitigate the negative effects on crop productivity. Recent literature suggests promising approaches in cultivating smart crops for salinity and drought tolerance. The use of halophytes and associated microbes shows potential for both stress mitigation and soil reclamation. Advanced omics tools provide deeper insights into the interactions among halophytes, microbiomes, and the rhizosphere habitat. Mechanism-based evidence supports the application of halophytic plants and their associated microbial communities in bio-saline agriculture.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Sripati Abhiram Sahoo, Reema Devi Singh, Jayant Kulkarni, Granthali Sanket Kamble, Manish Pandey, Satish Balkrishna Verulkar, Ashish Kumar Srivastava
Summary: This study investigated the efficacy of thiourea for ameliorating potassium-deficiency symptoms in soybean varieties. It was found that thiourea could significantly improve phenotype under potassium-deficient conditions by upregulating the expression of GmAKT2/3 and increasing enzymatic antioxidant activities. This suggests that thiourea can enhance soybean tolerance to potassium deficiency.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Seyed Morteza Zahedi, Marjan Sadat Hosseini, Mahdieh Karimi, Rahmatollah Gholami, Mojtaba Amini, Mostafa Abdelrahman, Lam-Son Phan Tran
Summary: Chitosan-based metal complexes, especially CH-Fe, have positive effects on pomegranate plants under drought stress by increasing chlorophyll content, microelement uptake, antioxidant enzyme activity, and improving fruit nutritional qualities.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Review
Horticulture
Suprasanna Penna, Shri Mohan Jain
Summary: Fruit species are important for nutrition and health security as they provide micronutrients, antioxidants, and bioactive phytoconstituents, making fruit-based products functional foods for the present and future. Conventional breeding methods have improved fruit quality, aroma, antioxidants, yield, and nutritional traits, but climate change and other challenges require novel strategies. Biotechnological research in fruit crops offers opportunities for large-scale multiplication of elite clones, in vitro, mutagenesis, and genetic transformation. Advanced molecular methods, such as GWAS and genomic selection, are paving the way for trait-based improvement in fruit plant species for enhanced nutritional quality and agronomic performance.
