Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 19, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/ijms19071910
Keywords
Dichotomomyces cejpii; transcriptome; gliotoxin biosynthesis; glutathione S-transferase; aldehyde reductase
Funding
- Science and Technology Program of Guangzhou, China [201607020018]
- Science and Technology Planning Project of Guangdong Province [2015A030302061, 2016A020222022]
- Team Project of Natural Science Foundation of Guangdong Province [2016A030312014]
- National Natural Science Foundation of China [31500037, 31272087]
- Natural Science Foundation of Guangdong Province [2015A030310103]
- Guangdong Provincial Innovative Development of Marine Economy Regional Demonstration Projects [GD2012-D01-002]
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Gliotoxin, produced by fungi, is an epipolythiodioxopiperazine (ETP) toxin with bioactivities such as anti-liver fibrosis, antitumor, antifungus, antivirus, antioxidation, and immunoregulation. Recently, cytotoxic gliotoxins were isolated from a deep-sea-derived fungus, Dichotomomyces cejpii. However, the biosynthetic pathway for gliotoxins in D. cejpii remains unclear. In this study, the transcriptome of D. cejpii was sequenced using an Illumina Hiseq 2000. A total of 19,125 unigenes for D. cejpii were obtained from 9.73 GB of clean reads. Ten genes related to gliotoxin biosynthesis were annotated. The expression levels of gliotoxin-related genes were detected through quantitative real-time polymerase chain reaction (qRT-PCR). The GliG gene, encoding a glutathione S-transferase (DC-GST); GliI, encoding an aminotransferase (DC-AI); and GliO, encoding an aldehyde reductase (DC-AR), were cloned and expressed, purified, and characterized. The results suggested the important roles of DC-GST, DC-AT, and DC-AR in the biosynthesis of gliotoxins. Our study on the genes related to gliotoxin biosynthesis establishes a molecular foundation for the wider application of gliotoxins from D. cejpii in the biomedical industry in the future.
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