Article
Biochemistry & Molecular Biology
Peng Zhao, Hui Xue, Xiangzhen Zhu, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: The study identified and characterized a novel Spodoptera litura P450 gene, CYP321B1, which plays a crucial role in tannin detoxification and metabolism in insects. By conducting RNA interference and metabolome analysis, it was confirmed that silencing CYP321B1 significantly affects weight gain under tannin induction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Peng Zhao, Hui Xue, Xiangzhen Zhu, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: In this study, a P450 gene CYP6AB12 was identified and characterized from Spodoptera litura, an insect species. The gene was found to play an important role in the detoxification and metabolism of gossypol, a plant allelochemical. Silencing the gene resulted in lowered expression levels and increased sensitivity to gossypol. These findings confirm the broad detoxification and metabolism abilities of P450s and provide a molecular basis for further research on detoxification metabolism and pest control.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wen-Tao Hou, Christian Staehelin, Mohammed Esmail Abdalla Elzaki, Muhammad Hafeez, Yu-Sen Luo, Rui-Long Wang
Summary: Spodoptera litura is a widely distributed, highly polyphagous pest that has developed resistance to various insecticides. This study found that elevated cytochrome P450 monooxygenase enzyme activity, particularly the overexpression of the novel gene CYP6AE68, in populations with resistance to indoxacarb may play a significant role in detoxification processes.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Li Shi, Wenlin Li, Yating Dong, Yao Shi, Yuliang Zhou, Xiaolan Liao
Summary: This study identified two transcripts of Spodoptera litura CPR (SlCPR-X1 and SlCPR-X2) and found that SlCPR-X1 plays a major role in indoxacarb resistance in S. litura. The study highlights the importance of insect CPR in xenobiotics detoxification and resistance development, suggesting CPR as a potential target for insecticide resistance management through RNAi or CRISPR/Cas.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Agronomy
Zhenzhen Zhao, Lan Zhang, Yanning Zhang, Liangang Mao, Lizhen Zhu, Xingang Liu, Hongyun Jiang
Summary: The study investigated the enzymatic response of cytochrome P450 monooxygenase (CYP) and glutathione S-transferase (GST) genes after CPT treatment in the fat body cells of Spodoptera exigua. The effects of CPT on the growth and development of S. exigua larvae were also examined. The relative expression levels of selected CYP and GST genes changed significantly during the CPT treatment period, suggesting their involvement in CPT metabolism.
Article
Agriculture, Multidisciplinary
Bi-Yun Zhang, Feng-Qi Li, Cheng Qu, Youssef Dewer, Yue-Jun Fu, Chen Luo
Summary: In this study, researchers identified genes related to sex pheromone biosynthesis in the Noctuid moths Spodoptera litura and Spodoptera exigua. They found that only the genes SlitDes5 and SexiDes5 were significantly overexpressed in the sex pheromone glands of these moths. Functional analysis of the SlitDes5 gene showed that its expression level could be reduced by 49.42% through RNA interference. These findings contribute to a better understanding of the key genes involved in sex pheromone biosynthesis and provide potential gene targets for pest control strategies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Han Wu, Dan Zhao, Xiao-Chang Guo, Zhao-Rui Liu, Rui-Jun Li, Xiu-Jun Lu, Wei Guo
Summary: Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects. Putative Group V CDAs were identified and characterized from beet armyworm larvae. The expression of these CDAs was found to be regulated by 20-hydroxyecdysone and juvenile hormone analog. Silencing of Group V CDAs resulted in compacted and evenly distributed intestinal wall cell layer, fragmented or disappeared vesicles, scarce PM structure, and loose and chaotic chitin microfilament structure. These results indicate the essential role of Group V CDAs in the growth and structuring of the midgut in beet armyworm larvae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Melanie Nolden, Robert Velten, Mark J. I. Paine, Ralf Nauen
Summary: Resistance to common pyrethroids, such as deltamethrin and permethrin, is widespread in the malaria mosquito Anopheles funestus due to upregulated cytochrome P450 monooxygenases. The duplicated genes CYP6P9a and CYP6P9b are highly upregulated in the pyrethroid resistant strain and have been shown to metabolize various pyrethroids. Although CYP6P9 variants interact with transfluthrin, their metabolic capacity to degrade transfluthrin is low, explaining the lack of resistance observed. Therefore, transfluthrin is an important resistance-breaking pyrethroid in controlling An. funestus.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Haiyuan Teng, Yayun Zuo, Jing Yuan, Jeffrey A. Fabrick, Yidong Wu, Yihua Yang
Summary: The beet armyworm, a global agricultural pest, has developed resistance to insecticides such as chlorantraniliprole and emamectin benzoate. Mutations in specific genes, such as SeRyR and CYP9A186, have been found to contribute to resistance. However, the resistance mechanisms may vary for different insecticides. Therefore, the use of insecticides with different modes of action and other integrated pest management strategies are necessary to effectively control the beet armyworm in China.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhixian Zhang, Xianpeng Song, Hongyan Hu, Dan Wang, Jixiang Chen, Yajie Ma, Xiaoyan Ma, Xiangliang Ren, Yan Ma
Summary: This study investigates the impact of double-stranded RNAs (dsRNAs) on the susceptibility of Spodoptera exigua larvae to Lufenuron (LUF). The researchers found that regulating the expression of genes associated with chitin biosynthesis and degradation can enhance the insecticidal activity of LUF against S. exigua. Additionally, the combined application of RNAi and LUF has significant effects on chitin content and biological response in S. exigua.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhixian Zhang, Dan Wang, Yongpan Shan, Jixiang Chen, Hongyan Hu, Xianpeng Song, Xiaoyan Ma, Xiangliang Ren, Yan Ma
Summary: Lufenuron and Methoxyfenozide are effective in controlling the crop pest Spodoptera exigua, but the pest has developed resistance to these chemicals possibly through cytochrome P450 monooxygenases. However, a mixture of Lufenuron and Methoxyfenozide remains highly insecticidal.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Xueke Gao, Xiangzhen Zhu, Chuanpeng Wang, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Junyu Luo, Jinjie Cui
Summary: This study characterized AgoCYP6CY19, a CYP gene in Aphis gossypii, and found that its expression is related to developmental stages and host specialization. Silencing AgoCYP6CY19 increased mortality and reduced fecundity in aphids. Additionally, AgoCYP6CY19 was induced by certain plant substances. These findings highlight the importance of AgoCYP6CY19 in detoxification and metabolism processes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agronomy
Yonglei Zhang, Runnan Yu, Jing Tang, Liheng Du, Yihan Wang, Jiatao Wang, Linsu Liu, Shanshan Gao, Bin Li
Summary: Three insect cytochrome P450 monooxygenase genes (TcCYP4Q3, TcCYP4Q5, and TcCYP4Q7) in Tribolium castaneum are regulated by the CncC signaling pathway and play a role in the susceptibility of the beetles to eugenol, a phytochemical. These findings have implications for the development of novel therapeutics to control pests.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biology
Yanwei Ruan, Xinxian Liu, Changwei Gong, Yuming Zhang, Litao Shen, Hasnain Ali, Yanyan Huang, Xuegui Wang
Summary: The P450 enzyme system plays a crucial role in the resistance development of the white-backed planthoppers, with CYP6CS3 identified as a main gene. Analysis of gene expression levels and RNA interference experiments confirmed the mechanism of action of CYP6CS3 in pesticide resistance development.
Article
Biochemistry & Molecular Biology
Tianxiang Xiao, Kai Lu
Summary: The common cutworm, Spodoptera litura, has developed resistance to commonly used insecticides. Two P450 enzymes, CYP6AE43 and CYP6AE48, play a crucial role in detoxifying pyrethroids and promoting resistance development in S. litura.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)