Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 437, Issue -, Pages 30-37Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijms.2018.06.006
Keywords
Multiplexed; MS/MS; Lipid; MALDI; Imaging mass spectrometry
Funding
- National Institutes of Health/National Institute of General Medical Sciences [5P41 GM103391-05]
- National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases [F32 FDK105841A]
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Tandem mass spectrometry (MS/MS) is often used to identify lipids in matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) workflows. The molecular specificity afforded by MS/MS is crucial on MALDI time-of-flight (TOF) platforms that generally lack high resolution accurate mass measurement capabilities. Unfortunately, imaging MS/MS workflows generally only monitor a single precursor ion over the imaged area, limiting the throughput of this methodology. Herein, we demonstrate that multiple TOF/TOF events performed in each laser shot can be used to improve the throughput of imaging MS/MS. This is shown to enable the simultaneous identification of multiple phos-phatidylcholine lipids in rat brain tissue. Uniquely, the separation in time achieved for the precursor ions in the TOF-1 region of the instrument is maintained for the fragment ions as they are analyzed in TOF-2, allowing for the differentiation of fragment ions of the exact same m/z derived from different precursor ions (e.g., the m/z 163 fragment ion from precursor ion m/z 772.5 is easily distinguished from the m/z 163 fragment ion from precursor ion m/z 826.5). This multiplexed imaging MS/MS approach allows for the acquisition of complete fragment ion spectra for multiple precursor ions per laser shot. (C) 2018 Elsevier B.V. All rights reserved.
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