Journal
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH
Volume 12, Issue 5, Pages 749-753Publisher
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.1007/s41742-018-0118-7
Keywords
M. oxyfera; M. nitroreducens; NC10; Anaerobic methanotroph
Categories
Funding
- JSPS KAKENHI [25701010, 15K12225, 16H02975]
- Grants-in-Aid for Scientific Research [16H02975, 15K12225, 25701010] Funding Source: KAKEN
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The growth rate of microorganisms that facilitate the denitrifying anaerobic methane oxidation (DAMO) reaction is slow; thus, establishment of appropriate cultivation conditions for these microorganisms is necessary. In this study, the effect of copper (Cu) and pyrroloquinoline quinone (PQQ) concentrations on the nitrite- and nitrate-dependent DAMO activities of these microorganisms were evaluated using an upflow-type continuous cultivation system. The study confirmed that 6 A mu M Cu and 2 nM PQQ had a positive effect on nitrite-dependent DAMO, but had little effect on nitrate-dependent DAMO. Conventional basal medium does not contain PQQ; therefore, supplementation of basal medium with PQQ could be an effective method for the cultivation of bacteria that facilitate nitrite-dependent DAMO.
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