Review
Biochemistry & Molecular Biology
Ping Zheng
Summary: Pluripotent stem cells have wide applications, but the mechanisms of maintaining genome stability in these cells are not clear.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Food Science & Technology
Yanyan Yi, Zhili Hao, Panpan Sun, Kuohai Fan, Wei Yin, Jianhua Guo, Xiaozhong Zheng, Na Sun, Hongquan Li
Summary: This study reveals that Scu can protect ovarian granulosa cells from ZEA-induced damage by regulating multiple targets and pathways, and possibly by inhibiting apoptosis of GCs.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Biology
Liang Liu, Jianyu Ma, Zongyou Wei, Yingnan Yang, Dongxu Li, Yongjie Wan
Summary: In this study, ZEA treatment was found to promote apoptosis and oxidative stress in goat granulosa cells (GCs), inhibit cell proliferation, and arrest the cell cycle. RNA-Seq analysis revealed altered expression of copper transport-related genes, leading to copper accumulation in GCs. These findings provide new insights into the relationship between ZEA toxicity and copper accumulation.
Article
Cell Biology
Chunli Sha, Lu Chen, Li Lin, Taoqiong Li, Hong Wei, Meiling Yang, Wujiang Gao, Dan Zhao, Qi Chen, Yueqin Liu, Xiaofang Chen, Wenlin Xu, Yuefeng Li, Xiaolan Zhu
Summary: TRDMT1 plays a crucial role in repairing DNA damage caused by reactive oxygen species and regulates granulosa cell apoptosis in premature ovarian failure.
Article
Biochemistry & Molecular Biology
Yingnan Yang, Yu Cai, Jinjing Guo, Keke Dai, Liang Liu, Zili Chen, Feng Wang, Mingtian Deng
Summary: KDM5B is crucial for early embryo development and its expression is highly regulated by maternal factors. In this study, we investigated the role of KDM5B in granulosa cells (GCs) and found that KDM5B is involved in cell cycle regulation and DNA damage prevention in GCs. Knockdown of KDM5B led to a decrease in cell proliferation and an increase in apoptosis, as well as a blockage of GCs at the S phase. Furthermore, knockdown of KDM5B resulted in increased reactive oxygen species levels, mitochondrial depolarization, and DNA double-strand breaks. These effects were partially rescued by up-regulation of MTF1. Overall, our findings suggest that KDM5B plays a critical role in promoting cell cycle progression and preventing DNA damage in GCs through the up-regulation of MTF1.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Editorial Material
Genetics & Heredity
Yang Zheng, Hao Li, Xiaochen Bo, Hebing Chen
Summary: Ionizing radiation (IR) induces complex DNA damage and repair mechanisms at hierarchical chromatin structures, necessitating the study of radiobiology from a 3D-genomic perspective. Differences in IR damage and repair within the 3D genome may provide insights into variations in radiosensitivity.
TRENDS IN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Xiaohong Yao, Chaofan Wang, Weiran Yu, Longjie Sun, Zheng Lv, Xiaomei Xie, Shuang Tian, Lu Yan, Hua Zhang, Jiali Liu
Summary: This study found that SRSF1 plays an important role in mouse granulosa cells. Specific knockout of SRSF1 leads to inhibition of follicular development, decreased granulosa cell proliferation, and increased apoptosis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Medicine, Research & Experimental
Shihui Guo, Xiao Zhu, Ziyuan Huang, Chuzhong Wei, Jiaao Yu, Lin Zhang, Jinghua Feng, Mingdong Li, Zesong Li
Summary: Genetic instability can be caused by external factors and is related to intracellular damage. Research has shown the connection between genomic stability and tumors, which is a crucial factor in tumor treatment. The mechanisms of genomic stability and tumorigenesis development are complex, but researchers have been making progress in these areas. This review introduces various aspects related to genomic stability, tumor development drivers, characteristics of tumor cells, tumor metastasis, and tumor treatment. Immunotherapy plays a significant role in tumor treatment as it can effectively inhibit tumor spread and eliminate tumor cells. Advances in tumorigenesis development studies and discoveries in tumor metastasis provide new therapeutic techniques that can prevent tumor spread and improve the cure rate of tumors.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Food Science & Technology
Yifeng Zhu, Heng Wang, Jianping Wang, Shunshun Han, Yao Zhang, Menggen Ma, Qing Zhu, Keying Zhang, Huadong Yin
Summary: This study investigated the effects of Zearalenone (ZEA) on chicken granulosa cells and found that ZEA inhibited cell growth by inducing apoptosis and promoting autophagy. Autophagy delayed apoptosis in ZEA-treated cells, reducing cytotoxicity. The results also showed that ZEA stimulated autophagy through PI3K-AKT-mTOR and MAPK signaling pathways in chicken granulosa cells.
Article
Toxicology
Wei Song, Yu-Ting Qiu, Xiao-Zhen Li, Qing-Yuan Sun, Lei-Ning Chen
Summary: In this study, the effects of 4-vinylcyclohexene diepoxide (VCD) exposure on human granulosa cells were investigated using a human granulosa cell line (SVOG). The results showed that VCD exposure had toxic effects on SVOG cells, leading to excessive accumulation of intracellular ROS, DNA damage, altered gene expression related to apoptosis and oxidative stress, and inhibited proliferative capacity of granulosa cells, resulting in increased cell apoptosis.
TOXICOLOGY IN VITRO
(2023)
Article
Agriculture, Dairy & Animal Science
Xiaoya Li, Huali Chen, Zelin Zhang, Jiaxin Duan, Rongmao Hua, Xiaodi Li, Li Yang, Jianyong Cheng, Qingwang Li
Summary: This study investigated the therapeutic effect of isorhamnetin on ZEA-induced damage in porcine ovarian granulosa cells and elucidated its molecular mechanism. The results showed that isorhamnetin protected ovarian granulosa cells from ZEA-induced damage, promoting proliferation and alleviating apoptosis, endoplasmic reticulum stress, oxidative stress, and steroidogenesis disorder.
Article
Medicine, Research & Experimental
Qing Yin, Ya-Jie Zhao, Wei-Jie Ni, Tao-Tao Tang, Yao Wang, Jing-Yuan Cao, Di Yin, Yi Wen, Zuo-Lin Li, Yi-Lin Zhang, Wei Jiang, Yue Zhang, Xiao-Yu Lu, Ai-Qing Zhang, Wei-Hua Gan, Lin-Li Lv, Bi-Cheng Liu, Bin Wang
Summary: This study found that miR-155 overexpression was associated with cisplatin-induced acute kidney injury (cAKI), and inhibition of miR-155 expression could attenuate cAKI-induced renal damage and apoptosis. The study also revealed that miR-155 inhibition increased the expression of TRF1 and CDK12, thereby limiting telomeric dysfunction and genomic DNA damage.
Review
Cell Biology
Ming Tang, Emma Bolderson, Kenneth J. O'Byrne, Derek J. Richard
Summary: Hypoxia is associated with poor prognosis in cancer due to enhanced tumor malignancy and therapeutic resistance. The aggressiveness of tumors partially stems from hypoxia-induced genomic instability. Understanding how tumor hypoxia induces genomic instability is crucial for improving cancer therapeutics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Enrique Lin-Shiao, Wolfgang G. Pfeifer, Brian R. Shy, Mohammad Saffari Doost, Evelyn Chen, Vivasvan S. Vykunta, Jennifer R. Hamilton, Elizabeth C. Stahl, Diana M. Lopez, Cindy R. Sandoval Espinoza, Alexander E. Deyanov, Rachel J. Lew, Michael G. Poirer, Alexander Marson, Carlos E. Castro, Jennifer A. Doudna
Summary: DNA nanostructures are a promising tool for delivering molecular payloads to cells, but effectively delivering genetic material to the nucleus has been a challenge. This study successfully integrated gene material into cells using DNA nanostructures as HDR templates, with increased entry into the nucleus through CRISPR technology. These nanostructured templates showed lower toxicity and higher insertion efficiency compared to unstructured DNA templates.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Viktoria K. Wandt, Nicola Winkelbeiner, Julia Bornhorst, Barbara Witt, Stefanie Raschke, Luise Simon, Franziska Ebert, Anna P. Kipp, Tanja Schwerdtle
Summary: Neurons are post-mitotic cells in the brain and their integrity is crucial in preventing neurodegeneration. Oxidative stress from high oxygen consumption can lead to DNA damage and genome instability. Imbalances in brain trace element homeostasis, such as iron, copper, manganese, zinc, and selenium, can impair neuronal maintenance and increase oxidative stress.