Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 108, Issue -, Pages 916-926Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2017.11.001
Keywords
Sulfated polysaccharide; Red seaweed; Breast cancer; Cell proliferation; MCF-7
Funding
- Atomic Energy Commission of Syria (AECS)
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Hydrocolloids from seaweeds (phycocolloids) have interesting functional properties like antiproliferative activity. Marine algae consumptions are linked to law cancer incidences in countries that traditionally consume marine products. In this study, we have investigated water-soluble sulfated polysaccharides isolated from the red seaweed Laurencia papillosa and determined their chemical characteristics and biological activities on the human breast cancer cell line MCF-7. Total polysaccharides were extracted and fractionated from L. papillosa and characterized using FTIR-ATR and NMR spectrometry. In addition, their approximate molar mass was determined by GPC method. The chemical characterization of purified polysaccharides reveals the presence of sulfated polysaccharides differentially dispersed in the algal cell wall. They are the three types of carrageenan, kappa, iota and lambda carrageenans, named LP-W1, -W2 and -W3 respectively. Biological effects and cytotoxicity of the identified of the three sulfated polysaccharide fractions were evaluated in MCF-7 cell line. Our results showed a significant inhibition of MCF-7 cell viability by dose-dependent manner for cells exposed to LP-W2 and LP-W3 polysaccharides for 24 h. The mechanistic of LP fractions -mediated apoptosis in MCF-7 cells was demonstrated. The biological effects of L. papillosaSPs indicate that it may be a promising candidate for breast cancer prevention and therapy. (C) 2017 Elsevier B.V. All rights reserved.
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