Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 110, Issue -, Pages 140-149Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.11.126
Keywords
Amphotericin B; Carboxymethylated iota-carrageenan; Macrophages; Gelatin A; Targeted drug delivery; Candida glabrata
Funding
- Nanomission, Department of Science and Technology (DST), Government of India [SR/NM/PG-01/2015]
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Intercellular Candida glabrata infections are difficult to treat due to poor penetration of drugs into the fungal niche. Delivering amphotericin B (Amp B) into the macrophages where the pathogen inhabits is an effective solution. We are studying the macrophage targeting proficiency of L-carrageenan for the delivery of Amp B using gelatin A nanoparticles (GNPs). The choice of gelatin A was the outcome of in silica inspections where the amino functionalized polymer having the best docking score with Amp B was selected. We prepared a sustained release formulation of amp B loaded carboxymethyl L-carrageenan conjugated gelatin nanoparticles (CMC-Amp B-GNPs) with size 343 +/- 12 nm and -25 +/- 5.3 mV zeta potential. The formulations were found to be stable, biocompatible and non-haemolytic. Flow cytometry analysis showed 3 fold higher uptake of CMC-GNPs compared to the GNPs by RAW 264.7 cells. CMC-Amp B-GNPs showed enhanced antifungal activity than bare Amp B and Amp B-GNPs. (C) 2017 Elsevier B.V. All rights reserved.
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