Article
Food Science & Technology
Yongtao Zhang, Shiquan You, Dongdong Wang, Dan Zhao, Jiachan Zhang, Quan An, Meng Li, Changtao Wang
Summary: In this study, the molecular characteristics of fermented Dendrobium officinale polysaccharides (FDOP) by Lactobacillus delbrueckil bulgaricus were analyzed using FT-IR, GPC-LC, and SEM. FDOP showed a smaller molecular structure and better water solubility. The protective effect of FDOP on human skin fibroblasts (HSF) damaged by UV radiation was investigated, and it was found that FDOP improved the antioxidant capacity of HSF and reduced the breakdown of collagen, elastin, and hyaluronic acid. The antioxidative and antiaging properties of FDOP were explored, and it was observed that FDOP could up-regulate the Nrf2/Keapl and TGF-beta/Smads pathways to reduce oxidative damage and aging effects.
FOOD SCIENCE & NUTRITION
(2022)
Article
Plant Sciences
Zhenyu Liu, Weili Wang, Xueqin Li, Sha Tang, Dongwei Meng, Wenli Xia, Hong Wang, Yuzhang Wu, Xinyuan Zhou, Jingbo Zhang
Summary: Capsaicin can effectively alleviate renal fibrosis, possibly by suppressing the activation of myofibroblasts and protecting against the phenotypic alteration of tubular epithelial cells. This is mainly achieved through the inhibition of the TGF-beta 1-Smad2/3 signaling pathway.
Article
Biochemistry & Molecular Biology
Yan Yang, Li Fan, Ying Peng, Chongsheng Peng, Xiaobo Li
Summary: This research demonstrates that the alcohol-soluble polysaccharide-enriched fractions from Dendrobium officinale flowers have significant antidepressant effects. The novel alcohol-soluble polysaccharide, ASP, is mainly composed of various sugars and has the ability to regulate gut microbiota and short-chain fatty acids, as well as activate the BDNF-TrkB-CREB pathway, leading to a protective effect against neuronal apoptosis and maintenance of the serotonin system. These findings highlight the potential use of Dendrobium officinale flowers as a rich source of polysaccharides for depression treatment.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Pharmacology & Pharmacy
Jiangcheng Shu, Lizhi Hu, Yichen Wu, Long Chen, Kai Huang, Zhaohui Wang, Minglu Liang
Summary: This study reveals that daidzein has an anti-fibrotic effect in myocardial fibrosis by reducing the activation of cardiac fibroblasts induced by TGF-beta 1, partially through regulating the TGF-beta 1/SMAD2/3 signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Environmental Sciences
Seung A. Chae, Min Cheol Pyo, Hee Joon Yoo, Kwang-Won Lee
Summary: This study investigated the mechanism of OTA-induced liver fibrosis and found that OTA induces epithelial-mesenchymal transition (EMT) and hepatic fibrosis through the TGF-beta RI and Smad2/3 signaling pathway. The results showed that OTA exposure increases the expression of liver fibrosis-related markers and elevates damage indicators. Silencing Smad2/3 and inhibiting TGF-RI can reduce the expression of liver fibrosis-related markers.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jianwu Fan, Xin Zhang, Yaping Jiang, Li Chen, Minjie Sheng, Yihui Chen
Summary: The silencing of SPARC in primary human pterygium fibroblasts (HPFs) led to inhibited expression of fibrotic markers, reduced cell migration and contractile phenotype, but had no effect on cell proliferation and apoptosis. These findings further support the key role of SPARC in the fibrotic process of pterygium.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Environmental Sciences
Alhanouf A. Aljohani, Yasmeen S. Alqarni, Maram N. Alrashidi, Maha H. Aljuhani, Shaimaa A. Shehata, Mohamed K. El-Kherbetawy, Kousalya Prabahar, Reem Alshaman, Abdullah Alattar, Ahmed M. N. Helaly, Hayam Ateyya, Ezzat A. Ismail, Sawsan A. Zaitone
Summary: This study assessed the nephrotoxic effect of leflunomide (LEFN) in mice and explored the role of TGF beta-stimulated p53/SMAD2/3 signaling. The results showed that chronic administration of LEFN led to increased levels of urea and creatinine in mice, as well as significant histopathological manifestations in kidney tissues. Western blot analysis also indicated upregulation of p-p53/Smad2/3 proteins. Therefore, LEFN-induced nephrotoxicity may be mediated through the TGF beta-stimulated p53/SMAD2/3 signaling pathway and induction of glomerular and tubular apoptosis.
Article
Plant Sciences
Xiao Chen, Min Wei, Guo-Dong Li, Qi-Liang Sun, Jia-Qi Fan, Jun-Yi Li, Chun-Mei Yun, Dao-Ming Liu, Hong Shi, Yi-Qing Qu
Summary: The study aims to investigate the therapeutic effects of YPF on pulmonary fibrosis. It was found that YPF may have significant therapeutic potential for pulmonary fibrosis by targeting various compounds and pathways, possibly attributed to its ability to up-regulate CAV1 expression and inhibit pulmonary fibrosis through modulation of the TGF-beta 1/Smad2 signaling pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2024)
Article
Plant Sciences
Yuanyuan Li, Wenguang Qin, Qiuling Liang, Jiamin Zeng, Qiong Yang, Yuqin Chen, Jian Wang, Wenju Lu
Summary: This study aimed to investigate the therapeutic efficacy of the Traditional Chinese Medicine formulation "Bufei huoxue" on IPF and its mechanisms. The results showed that "Bufei huoxue" can alleviate pulmonary fibrosis, improve lung function, reduce inflammation, and inhibit EMT and ECM production by inhibiting the TGF-beta 1/Smad2/3 signaling pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Oncology
Nuria Eritja, Raul Navaridas, Anna Ruiz-Mitjana, Maria Vidal-Sabanes, Joaquim Egea, Mario Encinas, Xavier Matias-Guiu, Xavier Dolcet
Summary: PTEN deficiency leads to SMAD2/3 activation, acting as a barrier to tumor development triggered by PTEN loss. TGF-beta plays dual roles as a tumor suppressor in premalignant cells and a tumor promoter in cancerous cells, influenced by different cellular contexts. The PI3K/PTEN/AKT and TGF-beta/SMAD pathways are crucial in maintaining epithelial cell homeostasis, with disruption contributing to endometrial carcinogenesis.
Article
Cell Biology
Zhen Tian, Yan Zhang, Xueman Lyu
Summary: This study reveals that KLF5 may induce myocardial damage and fibrosis in MI by modulating the miR-27a and GFPT2/TGF-beta/Smad2/3 pathway. Inhibition of KLF5 with ML264 and intervention with miR-27a can help reduce cell and tissue damage in MI.
Article
Plant Sciences
Shuyu Li, Qixin Yang, Feilong Chen, Linhua Tian, Jinhai Huo, Yanli Meng, Qingfa Tang, Weiming Wang
Summary: This study investigates the antipulmonary fibrosis mechanism of Pheretima protein and its possible cell signaling pathways. The results demonstrate that Pheretima protein can inhibit epithelial-mesenchymal transition, reduce inflammation, and alleviate BLM-induced pulmonary fibrosis in a mouse model. The preliminary mechanistic studies suggest that Pheretima protein exerts its biological activity by downregulating the TGF-β1/Smad2/3 pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Plant Sciences
Jing Liu, Dongyang Gao, Qi Ding, Binbin Zhang, Wenxiang Zhu, Yuanyuan Shi
Summary: The study proved that Sparganii Rhizoma (SR) has significant therapeutic effects against pulmonary fibrosis (PF) by inhibiting fibroblast differentiation and epithelial-mesenchymal transition. This provides a new idea and approach for the treatment of PF with traditional Chinese medicine.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Ying Wen, Xiuli Zhang, Lihui Wei, Meizhu Wu, Ying Cheng, Huifang Zheng, Aling Shen, Changgeng Fu, Farman Ali, Linzi Long, Yao Lu, Jiapeng Li, Jun Peng
Summary: This study investigated the effects of gastrodin on hypertensive renal injury and its underlying mechanisms using network pharmacology analysis and validation in vivo and in vitro. The results showed that gastrodin can alleviate renal injury and pathological changes in spontaneously hypertensive rats, as well as inhibit renal fibrosis. Additionally, gastrodin exerted its effects by suppressing the activation of the TGF-beta 1/Smad2/3 signaling pathway.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Xiaoyang Zhang, Yanfeng Yan, Yunxia Lv, Xin Li, Leiyao Chen, Zihui Huang, Junbo Zhou, Yong Wang, Xiaoming Wang, Xu Wang, Hongwei Gu
Summary: Urinary tract infections (UTI) caused by uropathogenic Escherichia coli (UPEC) can be attenuated by Dendrobium officinale polysaccharides (DOPs), which possess anti-inflammatory properties and inhibit the activation of NLRP3 pathway and ROS signal. DOPs effectively mitigate UPEC-induced pyroptosis in macrophage cells by reducing cell damage and pro-inflammatory cytokine secretion.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)