Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 117, Issue -, Pages 696-703Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.05.171
Keywords
Oxyalkylation; Low-melting-point agarose; Electrophoresis
Funding
- Public Science and Technology Research Funds projects of Ocean [201305015-2, 201505026-5]
- Major Protect on the Integration of Industry and Education of Fujian Province [2015N5005]
- Science and Technology Plan Project of Quanzhou City [2016N055]
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In this study, agarose was chemically modified with ethylene oxide, 1,2-epoxypropane and 1,2-epoxybutane, to prepare hydroxyethyl agarose (HEAG), hydroxypropyl agarose (HPAG), and hydroxybutyl agarose (HBAG). The structures and properties of the products were characterized by Fourier transform infrared spectroscopy (FTIR), H-1 NMR spectroscopy, X-ray diffractometry (XRD), rheology measurement and gel electrophoresis. The results showed that the gelling temperature (T-g) of HEAG, HPAG, and HBAG were decreased to 28.3 degrees C, 29.0 degrees C and 28.7 degrees C respectively, with the melting temperature (T-m) simultaneously declined to 64.2 degrees C, 63.8 degrees C and 63.0 degrees C, respectively. Meanwhile, the gel strength of HBAG, HEAG, and HPAG were dramatically reduced to 194 g/cm(2), 312 g/cm(2) and 279 g/cm(2), respectively. Furthermore, HEAG and HPAG showed excellent separation efficiency which is in accordance with commercialized low-melting-point (LMP) agarose (Amersco 0815). These results indicated that oxyalkylation is a promising and economical method for the preparation of LMP agarose. (C) 2018 Published by Elsevier B.V.
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