Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 112, Issue -, Pages 405-412Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.01.190
Keywords
Quercetin; Xanthine oxidase; Superoxide anion
Funding
- National Natural Science Foundation of China [31460422, 31060210]
- Natural Science Foundation of Jiangxi Province [20171BAB204029, 20143ACB20006, 20142BAB204001]
- State Key Laboratory of Food Science and Technology [SKLF-ZZB-201707, SKLF-ZZA-201612, SKLF-KF-201607]
- Foundation of Jiangxi Provincial Office of Education [GJJ150187]
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Quercetin, one of the most abundant flavonoid in the daily diet, was found to reversibly inhibit the generation of uric acid and superoxide radicals (O-2(-))catalyzed by xanthine oxidase (XOD) in a mixed-type manner with IC50 values of (2.74 +/- 0.04) x 10(-6) and (2.90 +/- 0.03) x 10(-6) mol L-1, respectively, and the inhibition of quercetin on O-2(-) generation may be ascribed to the reduced form of XOD by a ping-pong mechanism. XOD had one high affinity binding site for quercetin with a binding constant of 428 x 10(4)L mol(-1) at 298 K, and the binding process was predominately driven by van der Waals forces and hydrogen bonds on account of the negative enthalpy and entropy changes. Moreover, molecular docking confirmed that the binding site for quercetin located in the isoalloxazine ring of the flavin adenine dinucleotide (FAD) domain of XOD, then the diffusion of O-2(-) out of the FAD site was blocked in favor of another electron transferred from FADH(2) to O-2(-) to form hydrogen peroxide (H2O2). This study may clarify the role of quercetin on inhibiting XOD catalysis and provide a potential nutritional supplement for preventing gout and peroxidative damage. (C) 2018 Elsevier B.V. All rights reserved.
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