Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 115, Issue -, Pages 900-906Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.04.138
Keywords
Calcium alginate beads; Tannase; Fourier-transform infrared spectroscopy; Differential scanning calorimetry; X-ray diffraction
Funding
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) [88881.131854/2016-01]
- COST Action Multi-Functional Nano-Carbon Composite Materials Network (Multi Comp) [CA15107]
- Short Term Scientific Missions (STSMs) [CA15107, 40735]
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Incorporating enzymes into calcium alginate beads is an effective method to immobilise them and to preserve, at the same time, their catalytic activity. Sodium alginate was mixed with Aspergillus ficuum tannase in aqueous solution, and tannase-loaded calcium alginate beads were prepared using a simple droplet-based microfluidic system. Extensive experimental analysis was carried out to characterise the samples. Microscopic imaging revealed morphological differences between the surfaces of bare alginate matrix and tannase-loaded alginate beads. Thermal analysis allowed assessing the hydration contents of alginate and revealed the presence of tannase entrapped in the loaded beads, which was confirmed by vibrational spectroscopy. X-ray diffraction allowed us to conclude that alginate of tannase-loaded beads is not crystalline, which would make them suitable as carriers for possible controlled release. Moreover, they could be used in food applications to improve tea quality or clarify juices. (C) 2018 Elsevier B.V. All rights reserved.
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