Article
Microbiology
Yuqi Gao, Mingze Niu, Xiaohui Yu, Tingting Bao, Zhaowei Wu, Xin Zhao
Summary: A newly identified EPS-synthetic gene cluster in Lactiplantibacillus plantarum plays a crucial role in enhancing biofilm formation ability, tolerance to harsh environmental stresses, adhesion to intestinal epithelial cells, as well as attenuating inflammation and inhibiting pathogenic bacteria adhesion. This finding highlights the significance of mobile EPS-synthetic elements in the probiotic activity of L. plantarum and provides insights for probiotic bacteria engineering.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Mustafa Tahsin Yilmaz, Humeyra Ispirli, Osman Taylan, Mohammed Alamoudi, Enes Dertli
Summary: A slimy-mucinous-type colony of EPS-producing Weissella cibaria PDER21 was isolated and identified. The EPS was found to be a glucan type homopolysaccharide composed of glucose monomers. It exhibited superior thermal stability and antioxidant activity, as well as good water binding capacity and solubility. The findings suggest that the glucan PDER21 has great potential for use in the food industry.
CARBOHYDRATE POLYMERS
(2022)
Article
Food Science & Technology
Soyoung Park, Kandasamy Saravanakumar, Anbazhagan Sathiyaseelan, SeonJu Park, Xiaowen Hu, Myeong-Hyeon Wang
Summary: The present study isolated and identified five lactic acid bacteria from Korean kimchi, with Weissella cibaria producing a high level of exopolysaccharide (EPS) compared to other isolates. The EPS from W. cibaria was found to be composed of glucose and galactose, linked through specific monosaccharides. It exhibited strong antioxidant activity and biocompatibility, making it a promising candidate for biomedical applications.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Palmira De Bellis, Massimo Ferrara, Anna Rita Bavaro, Vito Linsalata, Mariaelena Di Biase, Biagia Musio, Vito Gallo, Giuseppina Mule, Francesca Valerio
Summary: Lactic acid bacteria can produce exopolysaccharides (EPS) with technological and functional properties for the food sector. This study focused on the characterization of high EPS-producing strain W. cibaria C43-11 in the presence of sucrose, compared to a low-producing strain C2-32, and investigated the genetic regulatory elements responsible for the modulation of dextransucrase (dsr) gene expression. NMR analysis revealed the composition of the EPS produced by C43-11 strain, which consists of a linear scaffold with glycosidic linkages and branches. Molecular analysis showed differences in the regulatory regions of dsr genes between the two strains. The upregulation of dsr gene in C43-11 strain resulted in a high accumulation of EPS. This study provides a new perspective for investigating the regulatory mechanism of EPS production.
Article
Food Science & Technology
Avinash Kant Lakra, Mahesh Ramatchandirane, Sandeep Kumar, Kitlangki Suchiang, Venkatesan Arul
Summary: The probiotic bacteria Weissella cibaria MD2 was used to produce exopolysaccharide (EPS) with excellent antioxidant activities, which can increase oxidative stress tolerance and enhance the lifespan of C. elegans.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eiseul Kim, Seung-Min Yang, Dae-Hyun Jung, Hae-Yeong Kim
Summary: In this study, a novel approach combining mass spectrometry and machine learning techniques was used to accurately identify the important food-fermenting bacteria Weissella cibaria and W. confusa. Through protein analysis and statistical analysis, it was found that although these two bacterial species exhibited similar protein profiles, they could be differentiated and classified using machine learning methods. The combination of mass spectrometry and machine learning enables accurate microbial identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Carsten Nachtigall, Verena Hassler, Daniel Wefers, Harald Rohm, Doris Jaros
Summary: The study focuses on the techno-functionality of five dextrans from W. cibaria DSM14295, which were produced under different cultivation conditions. The dextrans differ in molecular mass, intrinsic viscosity, degree of branching, side chain length, and architecture. The stiffness of acid gels spiked with these dextrans increased linearly with dextran concentration. Dextrans from W. cibaria DSM14295 have a high potential due to their high production yield and ability to be tailored by fermentation conditions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Fisheries
Silas F. Eto, Dayanne C. Fernandes, Michelli I. G. Funnicelli, Joao V. C. Alecrim, Pedro G. Souza, Fabiola C. A. Carvalho, Marco A. A. Belo, Joao M. Pizauro
Summary: This study provides the first description of the ability of Nile tilapia microglia to form extracellular traps (ETs) in response to Weissella cibaria infection. The findings show that ETs formation occurred 6 hours after stimulation with lipopolysaccharide, shedding light on the mechanism of ETs formation in teleost fish microglia and W. cibaria's ability to infect these cells.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Ji Soo Lee, In Kyung Hyun, Hye-Jin Seo, Dahyun Song, Min Young Kim, Seok-Seong Kang
Summary: The study demonstrated that whey biotransformed by Weissella cibaria can reduce lipid accumulation and intracellular triglycerides in adipocytes, by suppressing the expression of adipogenic-related transcription factors and target genes.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Applied
Fan Zhang, Lin Wang, Zihao Zhang, Baodong Zheng, Yi Zhang, Lei Pan
Summary: The study investigates the structure and cryoprotective activity of the exopolysaccharide EPS821-1 produced by Weissella cibaria. EPS821-1 is found to have a novel glucan structure and exhibits excellent cryoprotective activities for Bifidobacterium longum subsp. longum F2. This research provides scientific evidence and insights for the potential application of EPS821-1 as a cryoprotectant in the food industry.
Article
Microbiology
Di Yao, Xiaoyu Wang, Lixue Ma, Mengna Wu, Lei Xu, Qiaoru Yu, Liyuan Zhang, Xiqun Zheng
Summary: This study demonstrated the antifungal activity of isolated Weissella cibaria BYL4.2 against Penicillium chrysogenum. The cell-free supernatant (CFS) produced by W. cibaria BYL4.2 showed pH-dependent and partly heat-sensitive antifungal activity, with lactic acid as the main component. D-tartaric acid, detected by non-targeted metabolomics analysis, was identified as the main antifungal substance of the CFS, which inhibited the growth of P. chrysogenum by down-regulating metabolites in the ABC transporters pathway.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Ye-Ji Jang, Hee-Min Gwon, Woo-Soo Jeong, Soo-Hwan Yeo, So-Young Kim
Summary: Weissella cibaria JW15 has demonstrated safety for use as a probiotic, with intrinsic antibiotic resistance genes, absence of acquired virulence genes, lack of hemolysis and production of toxic metabolites, making it a promising functional food ingredient in the market.
Article
Microbiology
Hee-seung Han, Haeji Yum, Young-Dan Cho, Sungtae Kim
Summary: This study aimed to examine the effects of Weissella cibaria Chonnam Medical University (CMU)-containing tablets on halitosis. The results showed that the use of tablets containing W. cibaria CMU can effectively reduce the concentration of volatile sulfur compounds, improve bad breath scores, and improve psychological indicators in patients with halitosis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Morten Kam Dahl Dueholm, Maaike Besteman, Emil Juel Zeuner, Marie Riisgaard-Jensen, Morten Eneberg Nielsen, Sofie Zacho Vestergaard, Soren Heidelbach, Nicolai Sundgaard Bekker, Per Halkjaer Nielsen
Summary: A study investigated the genomic potential for exopolysaccharide biosynthesis in bacterial species typical in activated sludge (AS) systems based on genome mining and gene synteny analyses. Putative gene clusters associated with the biosynthesis of various exopolysaccharides were identified in AS bacteria. This study provides a comprehensive overview of the genome-resolved potential for these exopolysaccharides in AS bacteria and contributes to a better understanding of EPS composition in wastewater treatment systems.
Article
Biotechnology & Applied Microbiology
G. Jiang, J. He, L. Gan, X. Li, Y. Tian
Summary: Lactiplantibacillus pentosus strain B8 isolated from Sichuan paocai was found to produce high levels of exopolysaccharides (EPS-B8) with desirable properties such as solubility, water holding capacity, oil holding capacity, emulsifying activity, and coagulating ability. EPS-B8 also demonstrated the ability to enhance the growth of rice seedlings. These findings suggest the potential use of EPS-B8 as a functional food ingredient and plant-growth regulator.
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)