Portable bioactive paper based genosensor incorporated with Zn-Ag nanoblooms for herpes detection at the point-of-care

The present work describes the fabrication of an electrochemical paper-based analytical device (EPAD) integrated with Zn-Ag nanoblooms for detection of herpes in human, caused by Herpes virus 5(HHV-5) DNA, at the point of care. The cyclic voltammetry(CV) was used for electrochemical detection of the HHV-5 DNA in infected patient samples. The EPAD exihibited optimum current response within 5 s at pH7.0 and 35 degrees C with two dynamic linear/working ranges, 113-10(3) and 3 x 10(5)-10(6) copies/mL and detection limit of 97 copies/mL. The device showed high selectivity, repeatability, and sensitivity. The device had many advantageous features such as portable, facile approach, economical and potential for commercialization. The proposed sensing scheme is highly feasible for future clinical detection of Herpes virus. (C) 2017 Elsevier B.V. All rights reserved.