Journal
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
Volume 101, Issue -, Pages 113-117Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biocel.2018.06.002
Keywords
Super-resolution microscopy; Fluorescence spectral imaging; Single-molecule spectroscopy
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Funding
- National Science Foundation [CBET-1055379, EEC-1530734]
- National Institutes of Health [R01EY026078]
- Northwestern University Innovative Initiative Incubator (I3) Award
- National Science Foundation Graduate Research Fellowship Program [1000231682]
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The recent surge in spectroscopic Single-Molecule Localization Microscopy (sSMLM) offers exciting new cap abilities for combining single molecule imaging and spectroscopic analysis. Through the synergistic integration of super-resolution optical microscopy and single-molecule spectroscopy, sSMLM offers combined strengths from both fields. By capturing the full spectra of single molecule fluorescent emissions, sSMLM can distinguish minute spectroscopic variations from individual fluorescent molecules while preserving nanoscopic spatial localization precision. It can significantly extend the coding space for multi-molecule super-resolution imaging. Furthermore, it has the potential to detect spectroscopic variations in fluorescence emission associated with molecular interactions, which further enables probing local chemical and biochemical inhomogeneities of the nano-en- vironments. In this review, we seek to explain the working principle of sSMIM technologies and the status of sSMLM techniques towards new super-resolution imaging applications.
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