Article
Dentistry, Oral Surgery & Medicine
Cristina Bucchi, Ana Bucchi, Paulina Martinez-Rodriguez
Summary: This study aimed to assess the impact of inflammatory status of the dental pulp and the sustained inflammatory environment on the biological characteristics of dental pulp stem cells (DPSCs). The results showed that the inflammatory status of the pulp significantly reduced the viability and mineralization capacity of DPSCs, while it did not affect the adhesion capacity to dentin or the secretion of proinflammatory cytokines. The inflammatory microenvironment had a significant impact on the secretion of interleukin 6.
JOURNAL OF ENDODONTICS
(2023)
Article
Biochemistry & Molecular Biology
Cristina- Bucchi, Ella Ohlsson, Josep Maria de Anta, Melanie Woelflick, Kerstin Galler, Maria Cristina Manzanares-Cespedes, Matthias Widbiller
Summary: Pluripotent stem cells derived from the amnion show the ability to adhere to dentin and mineralize in vitro, but exhibit unfavorable proliferation behavior and fail to undergo odontoblastic transition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Wen-Jin Chen, Jing Xie, Xi Lin, Ming-Hang Ou, Jun Zhou, Xiao-Lang Wei, Wen-Xia Chen
Summary: The study demonstrated that LPS-preconditioned sEVs can enhance the proliferation, migration, angiogenesis, and differentiation of mesenchymal stem cells, leading to structurally improved regenerated tissue similar to normal dental pulp in vivo. These findings suggest that LPS-preconditioned sEVs play a significant role in dental pulp regeneration.
JOURNAL OF ENDODONTICS
(2021)
Article
Biotechnology & Applied Microbiology
Nunthawan Nowwarote, Stephane Petit, Francois Come Ferre, Florent Dingli, Victor Laigle, Damarys Loew, Thanaphum Osathanon, Benjamin P. J. Fournier
Summary: The study identified the matrisome of ECM proteins derived from human DPSCs and found that it can regulate mineralization, even in cells which do not respond to mineralization assaults like GF.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Ehsaneh Azaryan, Samira Karbasi, Mansoore Saharkhiz, Mohammad Yahya Hanafi-Bojd, Asghar Zarban, Fariba Emadian Razavi, Mohsen Naseri
Summary: The study aimed to investigate the effects of human milk exosomes (HM-Exos) on lipopolysaccharide (LPS)-exposed human dental pulp stem cells (HDPSCs) in terms of viability, migration, and inflammatory responses. The results showed that HM-Exos could promote migration and proliferation of LPS-exposed HDPSCs and reduce the expression of inflammatory cytokines. These findings suggest that HM-Exos could be a promising candidate for pulpitis therapy.
Review
Cell Biology
Kyu Hwan Kwack, Hyeon-Woo Lee
Summary: Dental caries is a common disease that causes damage to teeth and pulp tissue. Dental pulp stem cells have multipotency, high proliferation rate, and immunosuppressive properties, making them ideal for regenerating damaged pulp tissue.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Jingjie Zeng, Kaili He, Ruotong Mai, Tian Lin, Rixia Wei, Jiaomei Nong, Yu Wu
Summary: This study evaluated the effects of exosomes derived from human umbilical cord mesenchymal stem cells and human dental pulp stem cells on lipopolysaccharide-induced inflammation in human dental pulp stem cells. The results showed that exosomes could reverse the inhibitory effect of lipopolysaccharide, suppress apoptosis, and reduce the expression of pro-inflammatory cytokines.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
M. M. Liu, W. T. Li, X. M. Xia, F. Wang, M. MacDougall, S. Chen
Summary: The translation above explains the process of dentineogenesis and the role of DSPP in tooth development. DSPP mutations can lead to hereditary dentine diseases, and DSPP plays a crucial role in dentine formation and odontoblast differentiation. Additionally, DSPP interacts with integrins and other molecules to regulate intracellular signaling and promote hydroxyapatite crystal growth and mineralization.
EUROPEAN CELLS & MATERIALS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Igor Paulino Mendes Soares, Giovana Anovazzi, Caroline Anselmi, Maria Luisa Leite, Debora Lopes Salles Scheffel, Diana Gabriela Soares, Carlos Alberto de Souza Costa, Josimeri Hebling
Summary: The study investigated the biological effects of treating EWSLs with resin infiltration components on odontoblast-like cells and human dental pulp cells. The results showed that RICs reduced cell viability, ALP activity, and MN formation, while upregulating inflammatory cytokines and downregulating mineralization-related genes. Among the RICs, the Etch component had the most detrimental effects.
Article
Cell & Tissue Engineering
Mohammed Zayed, Koichiro Iohara, Hideto Watanabe, Mami Ishikawa, Michiyo Tominaga, Misako Nakashima
Summary: The study found that hypoxia-preconditioned DPSCs (hpDPSCs) have advantages in proliferation rate and stemness, making them a potential effective source for pulp regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Edward J. Calabrese, Evgenios Agathokleous, Gaurav Dhawan, Rachna Kapoor, Vittorio Calabrese
Summary: This study represents the first assessment of hormetic dose responses by human dental pulp stem cells, focusing on cell renewal and differentiation. The study found that hormetic dose responses are commonly observed in this model, involving a wide range of chemicals including pharmaceuticals, dietary supplements/extracts from medicinal plants, and endogenous agents. The study evaluated the mechanistic foundations, study design considerations, and therapeutic implications of the hormetic dose responses in hDPSCs.
AGEING RESEARCH REVIEWS
(2022)
Review
Biochemistry & Molecular Biology
Konstantina Kritikou, Maria Greabu, Marina Imre, Daniela Miricescu, Alexandra Ripszky Totan, Marian Burcea, Iulia-Ioana Stanescu-Spinu, Tudor Spinu
Summary: Bacterial invasion causing inflammation leads to the release of various mediators from pulp tissue, with ILs and MMPs playing crucial roles. Biomarkers like ILs and MMPs can be detected through methods like ELISA, providing insights into the inflammatory response of dental pulp.
Article
Biochemistry & Molecular Biology
Duaa Abuarqoub, Nouf Mahmoud, Walhan Alshaer, Marwa Mohammad, Abed Alqader Ibrahim, Mairvat Al-Mrahleh, Mohammad Alnatour, Dana A. Alqudah, Ezaldeen Esawi, Abdalla Awidi
Summary: Gold nanoparticles (AuNPs) have been widely used in biological and biomedical applications. This study evaluated the effects of two different shapes of AuNPs, rods (AuNR-PEG) and spheres (AuNS-PEG), on dental pulp stem cells. The results showed that AuNS-PEG had a stimulatory effect on cell proliferation and lower toxicity compared to AuNR-PEG. Additionally, cells treated with AuNPs and cultured with fetal bovine serum showed higher proliferation rates and lower toxicity compared to serum-free media.
Review
Immunology
Fenyao Li, Xinxin Wang, Jin Shi, Shuting Wu, Wenbo Xing, Yan He
Summary: Dental pulp stem cells have strong immunomodulatory capabilities and can be used to treat inflammation-related diseases and autoimmune disorders. The mechanism of action is complex and may involve the regulation of immune cells through inflammatory immune-related signaling pathways.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Polymer Science
Bowen Liu, Chenjiao Zhang, Han Zhao, Jian Gao, Jingchao Hu
Summary: This study investigates the correction of PAX9 gene mutation using gene editing technology and chitosan hydrogel. The results show that chitosan hydrogel can efficiently deliver naked DNA into dental pulp stem cells and achieve higher correction efficiency compared to gene replacement. Moreover, the corrected PAX9 gene exhibits enhanced viability, differentiation capacity, and transcriptional activation ability, which has significant implications for treating hypodontia.
Article
Dentistry, Oral Surgery & Medicine
Reinhold Lang, Karl-Anton Hiller, Lena Kienboeck, Katrin Friedl, Karl-Heinz Friedl
Summary: This study evaluated the bond strength between zirconia frameworks and titanium bases using different composite resin luting agents with and without thermocycling and autoclaving. The results showed that MHA and PV5 had higher bond strength and were not significantly influenced by autoclaving.
JOURNAL OF PROSTHETIC DENTISTRY
(2022)
Article
Nutrition & Dietetics
Teresa Thim, Konstantin Johannes Scholz, Karl-Anton Hiller, Wolfgang Buchalla, Christian Kirschneck, Jonathan Fleiner, Johan Peter Woelber, Fabian Cieplik
Summary: This retrospective cross-sectional study aimed to evaluate the association between periodontal diseases and patient-specific risk factors, particularly LDL-C and 25OHD levels. The analysis of data from 163 patients found that higher RBL was significantly associated with male gender, older age, smoking, higher DMFT score, fewer teeth, and higher LDL-C levels, while higher 25OHD levels were associated with less RBL.
Article
Biochemistry & Molecular Biology
Martyna Smeda, Kerstin M. Galler, Melanie Woelflick, Andreas Rosendahl, Christoph Moehle, Beate Lenhardt, Wolfgang Buchalla, Matthias Widbiller
Summary: Both dental pulp and apical papilla are potential sources of stem cells for regenerative endodontic treatments. This study compared the molecular characteristics of dental pulp stem cells (DPSC) and stem cells from the apical papilla (SCAP). The results showed that both cell types expressed similar mesenchymal stem cell markers and had the ability to differentiate into various lineages. However, differences were observed in the profiles of secreted molecules and gene regulation. Overall, both DPSC and SCAP are suitable for dental pulp tissue engineering from a molecular biological perspective.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Denise Muehler, Xiaojun Mao, Stefan Czemmel, Janina Geissert, Christina Engesser, Karl-Anton Hiller, Matthias Widbiller, Tim Maisch, Wolfgang Buchalla, Ali Al-Ahmad, Fabian Cieplik
Summary: This study investigated the transcriptomic stress response in Streptococcus mutans after treatment with chlorhexidine digluconate (CHX). The results showed significant differential expression of genes and pathways involved in purine nucleotide synthesis, biofilm formation, transport systems, and stress responses. These findings provide insights into potential mechanisms that may lead to the development of resistance to CHX.
Article
Infectious Diseases
David L. Auer, Xiaojun Mao, Annette Carola Anderson, Denise Muehler, Annette Wittmer, Christiane von Ohle, Diana Wolff, Cornelia Frese, Karl-Anton Hiller, Tim Maisch, Wolfgang Buchalla, Elmar Hellwig, Ali Al-Ahmad, Fabian Cieplik
Summary: This study reveals that clinical isolates of early colonizers of dental plaque can phenotypically adapt to antiseptics such as chlorhexidine digluconate (CHX) upon repeated exposure. Some strains showed increased biofilm formation capacity and exhibited antibiotic resistance to tetracycline and erythromycin.
Article
Dentistry, Oral Surgery & Medicine
Konstantin J. Scholz, Woocheol Sim, Silvio Bopp, Karl-Anton Hiller, Kerstin M. Galler, Wolfgang Buchalla, Matthias Widbiller
Summary: The aim of this study was to investigate the impact of cavity cleaning and conditioning on the marginal integrity of post-endodontic composite restorations. The results showed that thorough cleaning with alcohol can ensure acceptable marginal integrity, while additional pre-treatment did not significantly improve the outcome.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Review
Dentistry, Oral Surgery & Medicine
Matthias Widbiller, Helge Knuettel, Nastaran Meschi, Fernando Duran-Sindreu Terol
Summary: Regenerative endodontics using endodontic tissue engineering (ETE) approaches show promise for the treatment of pulp necrosis in permanent teeth. This systematic review compared the effectiveness of ETE with conventional endodontic treatments and found limited evidence supporting the benefits and high survival rates of ETE. However, methodological limitations and inconsistent outcomes highlight the need for more rigorous studies in this field.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Ella Ohlsson, Kerstin M. Galler, Matthias Widbiller
Summary: Efforts in tissue engineering to repair damaged dental pulp tissue have shown positive results, but clinical measures cannot distinguish between true regeneration and repair. Therefore, research models are crucial in understanding treatment outcomes and advancing dental pulp regeneration. This review provides information on various in vitro and in vivo models, discusses the differences between monolayer and three-dimensional cell cultures, presents a new in vivo transplantation model for dental pulp regeneration, and introduces different animal models used for in vivo research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Eva Paddenberg, Anne Forneck, Matthias Widbiller, Martyna Smeda, Jonathan Jantsch, Peter Proff, Christian Kirschneck, Agnes Schroeder
Summary: This study investigated the effect of melatonin on macrophages exposed to mechanical strain during orthodontic tooth movement. The results showed that melatonin can inhibit the expression of inflammatory factors and has an anti-inflammatory effect on macrophages. This effect may be mediated by the involvement of MT2 receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Steffen Moehring, Fabian Cieplik, Karl-Anton Hiller, Helga Ebensberger, Gerlinde Ferstl, Joshua Hermens, Melanie Zaparty, Ralph Witzgall, Ulrich Mansfeld, Wolfgang Buchalla, Konstantin Johannes Scholz
Summary: Knowledge of elemental composition is required to use animal teeth as substitutes for human teeth in research. Energy dispersive X-ray spectroscopy (EDX) enables determining local elemental compositions of inorganic and organic compounds without sample destruction. The elemental composition of enamel and dentin in murine teeth significantly differs from that in human and bovine teeth.
Article
Microbiology
Karl-Anton Hiller, Verena Wenzl, Eva-Maria Forster, Fabian Cieplik, Tim Maisch
Summary: Combination therapies are effective in preventing bacterial resistance to antibacterial approaches. This study aimed to determine the optimal effective concentration combination (OPECC) for binary application of antibacterial compounds. The checkerboard assay was used to evaluate the synergistic effects of different combinations of antiseptics and antibiotics against Escherichia coli. An OPECC was determined for most binary combinations, and this method can be applied to any system targeting pathogen eradication.
Article
Health Care Sciences & Services
Matthias Widbiller, Andreas Rosendahl, Ralf Schlichting, Christine Schuller, Benedikt Lingl, Karl-Anton Hiller, Wolfgang Buchalla, Kerstin M. Galler
Summary: The purpose of this study was to compare different activation methods for endodontic irrigation on irrigant penetration, smear layer removal, and dentin surface effects. The results showed that all tested activation methods removed a thicker smear layer compared to conventional needle irrigation. Additional activation of EDTA improved irrigant penetration but did not enhance smear layer removal. Moreover, the additional activation of EDTA and laser-based techniques caused surface disintegration of dentin. Overall, additional activation of EDTA offers no significant benefits but disrupts dentin surface, especially with laser-based activation resulting in undesirable destruction of root canal wall dentin.