Article
Microbiology
Alicia Macias-Valcayo, John-Jairo Aguilera-Correa, Antonio Broncano, Raul Parron, Alvaro Aunon, Joaquin Garcia-Canete, Antonio Blanco, Jaime Esteban
Summary: This study analyzed a large number of isolates of Gram-negative bacilli that cause prosthetic joint infection. The study includes biofilm development and antimicrobial susceptibility testing of both planktonic and sessile bacteria. The results obtained support the clinical knowledge about the treatment of these bacteria when biofilms are involved.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
M. Cristina Vazquez Guillamet, Christopher Damulira, Andrew Atkinson, Victoria J. Fraser, Scott Micek, Marin H. Kollef
Summary: The combination of aminoglycosides and β-lactams is more effective in reducing the incidence of infections caused by multidrug-resistant Gram-negative bacilli compared to β-lactams alone. This retrospective cohort study analyzed septic patients treated between 2010 and 2017, and found that the addition of aminoglycosides to β-lactams significantly decreased the risk of subsequent infections.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Orthopedics
Vikas M. Agashe, Rajeev Soman, Camilla Rodrigues, Anjali Shetty, Ayesha Sunavala, Sagar Raghuvanshi, Aditya Menon
Summary: In this retrospective study, the treatment outcomes of 26 patients with carbapenem-resistant Gram-negative infections were analyzed. A multi-disciplinary team approach was utilized, focusing on source control and preventing recurrence. The study found that by modifying treatment goals to salvage lives and achieve infection remission, the majority of patients' lives could be saved.
INTERNATIONAL ORTHOPAEDICS
(2022)
Article
Public, Environmental & Occupational Health
Gunasekaran Rameshkumar, Ranjithkumar Dhandapani, Prajna Lalitha, Siva Ganesa Karthikeyan Rajapandian, Velmurugan Palanivel, Sathiamoorthi Thangavelu, Abdullah A. Alyousef, Thamer Albalawi, Pravej Alam, Mohammad Zubair, Fayez M. Saleh, Fuad Abdullah Alatawi, Fohad M. Husain
Summary: In this study, the prevalence, antibacterial sensitivity patterns, and molecular characterization of MβL associated resistant genes in gram-negative bacteria isolated from ocular infections were investigated. The study found a high prevalence of MβL production in Pseudomonas aeruginosa, leading to high resistance to certain antibiotics. The bla(VIM) gene was also detected at a high rate in eye infections.
FRONTIERS IN PUBLIC HEALTH
(2022)
Review
Infectious Diseases
Kelly L. Maguigan, Mohammad H. Al-Shaer, Charles A. Peloquin
Summary: Beta-lactam antibiotics are the cornerstone for treating Gram-negative infections in critically ill patients, however, dosing adjustments may be necessary due to the unique physiology and increasing resistance rates in the ICU setting. Continuous infusions are often required to achieve desired pharmacokinetic/pharmacodynamic targets, especially when dealing with less-susceptible pathogens such as Pseudomonas aeruginosa.
Article
Infectious Diseases
Kwadwo Mponponsuo, Kevin A. Brown, Daniel J. Fridman, Jennie Johnstone, Bradley J. Langford, Samantha M. Lee, Derek R. MacFadden, Samir N. Patel, Kevin L. Schwartz, Nick Daneman
Summary: In this study, the clinical outcomes of highly bioavailable oral antibiotics were compared with less-bioavailable options in patients with gram-negative bloodstream infections. The results showed that patients who received highly bioavailable antibiotics had significantly better clinical outcomes compared to those who received less-bioavailable options.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Microbiology
Alecks Megxel S. Abordo, Mark B. Carascal, Roland Remenyi, Doralyn S. Dalisay, Jonel P. Saludes
Summary: In the clinically isolated Gram-negative bacilli in the Philippines, the co-occurrence of multiple β-lactamases, especially ESBL and metallo-β-lactamases, was observed. Through genetic analysis and phenotypic characterization, the presence of multi-class β-lactamases and cryptic metallo-β-lactamases in these resistant bacteria was revealed.
Article
Medicine, Research & Experimental
Xiaojuan Zhang, Shaoyan Qi, Xiaoguang Duan, Bing Han, Shuguang Zhang, Shaohua Liu, Haixu Wang, Haibo Zhang, Tongwen Sun
Summary: This study evaluated the effectiveness and safety of polymyxin B in the treatment of carbapenem-resistant Gram-negative bacilli infections. The results showed positive clinical outcomes, with a relatively high bacteria eradication rate but also significant mortality at 28 days. Adverse reactions, including nephrotoxicity, were observed in a portion of patients.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Infectious Diseases
Milo Gatti, Pier Giorgio Cojutti, Renato Pascale, Tommaso Tonetti, Cristiana Laici, Alessio Dell'Olio, Antonio Siniscalchi, Maddalena Giannella, Pierluigi Viale, Federico Pea
Summary: This study suggests that early targeting of continuous infusion beta-lactams at a C-ss/MIC ratio > 5 may be beneficial in preventing microbiological failure and resistance development in critically ill patients with documented Gram-negative infections.
Article
Infectious Diseases
Kang Sun, Wangping Li, Yu Li, Guangyu Li, Lei Pan, Faguang Jin
Summary: This study developed a scoring model to predict antibiotic-associated bronchopneumonia caused by Gram-negative bacteria. The model was able to clearly differentiate low-risk, moderate-risk, and high-risk patients, and it was validated in a separate cohort.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Multidisciplinary Sciences
Maria Dolores Alcantar-Curiel, Manuel Huerta-Cedeno, Ma Dolores Jarillo-Quijada, Catalina Gayosso-Vazquez, Jose Luis Fernandez-Vazquez, Maria Luisa Hernandez-Medela, Manuelita Zavala-Pineda, Miguel Angel Morales-Gil, Veronica Alejandra Hernandez-Guzman, Manuel Ismael Bolanos-Hernandez, Silvia Giono-Cerezo, Jose Ignacio Santos-Preciado
Summary: This study describes the incidence of bacteremia caused by Gram-negative ESKAPE bacilli during the COVID-19 pandemic and characterizes the clinical and microbiological findings, including antimicrobial resistance. The results show that these bacteria, especially Acinetobacter baumannii, are highly multidrug-resistant and associated with a high mortality rate in patients with COVID-19. The study highlights the important role of multidrug-resistant Gram-negative ESKAPE bacteria in causing bloodstream infections in healthcare settings and suggests that the COVID-19 pandemic has not had a significant impact on antimicrobial resistance rates in the short term.
Article
Microbiology
Rafael Ayerbe-Algaba, Nuria Bayo, Ester Verdu, Raquel Parra-Millan, Jesus Seco, Meritxell Teixido, Jeronimo Pachon, Ernest Giralt, Younes Smani
Summary: In this study, ten AOA-2 derivatives were synthesized and evaluated for their effects against Gram-negative bacilli infections. Among them, RW01 and RW06 showed promising potential in improving the inhibitory effect of AOA-2, specifically against Pseudomonas aeruginosa and Escherichia coli by reducing bacterial adherence to host cells while slightly improving the viability of infected cells. Additionally, RW01 and RW06 also potentiated the activity of colistin against Acinetobacter baumannii strain, highlighting their potential as effective antimicrobial agents.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Ayan Ali Ragueh, Mohamed Houmed Aboubaker, Sitani Idriss Mohamed, Jean-Marc Rolain, Seydina M. Diene
Summary: This study investigated the resistance rate to carbapenems among multidrug-resistant Gram-negative bacteria (MDR-GNB) in Djibouti and characterized their resistance mechanisms. The results showed that out of 256 isolates, 20 were resistant to carbapenems. Carbapenemase genes such as bla(OXA-48) and bla(NDM-5) were identified in the isolates.
Article
Infectious Diseases
Viveka Nordberg, Aina Iversen, Annika Tidell, Karolina Ininbergs, Christian G. Giske, Lars Naver
Summary: This study in Stockholm, Sweden, found lower incidence of GNB sepsis in neonates compared to previous reports and low occurrence of antibiotic resistance. The in-hospital mortality and 5-day case fatality rate were higher in GNB late-onset sepsis compared to uninfected controls but not in comparison to suspected sepsis.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Pasquale Maria Berrino, Milo Gatti, Matteo Rinaldi, Eugenio Brunocilla, Pierluigi Viale, Federico Pea
Summary: This study explores the relationship between pharmacokinetic/pharmacodynamic (PK/PD) target attainment of continuous infusion (CI) piperacillin-tazobactam or meropenem monotherapy and microbiological outcome in urological patients with Gram-negative infections. The results demonstrate that optimizing beta-lactam treatment through real-time therapeutic drug monitoring (TDM)-guided expert clinical pharmacological advice (ECPA) program can achieve microbiological eradication in most cases.
Article
Immunology
Arnau Antoli, Gemma Rocamora-Blanch, Mario Framil, Virginia Mas-Bosch, Sergio Navarro, Carla Bermudez, Sergio Martinez-Yelamos, Eva Dopico, Laura Calatayud, Nadia Garcia-Munoz, Luis Humberto Hernandez-Benitez, Antoni Riera-Mestre, Jordi Bas, Cristina Masuet-Aumatell, Raul Rigo-Bonnin, Francisco Morandeira, Xavier Solanich
Summary: The study found that SARS-CoV-2 vaccines are less effective in CVID phenotype and BCD patients compared to healthy individuals. Predictive factors of inadequate specific antibody response in CVID phenotype patients include low IgA values, low CD19+ peripheral B cells, low switched memory B cells, and a low CD4+/CD8+ ratio. Further studies are needed to understand the effectiveness of vaccines in immunocompromised patients and predictive factors of immune response failure.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Cristina El Haj, Eva Benavent, Yanik Sierra, Laura Soldevila, Raul Rigo-Bonnin, Benjamin Torrejon, Joan Gomez-Junyent, Irantzu Rossello, Oscar Murillo
Summary: The anti-biofilm efficacy of dalbavancin (DAL) was significantly improved by adding rifampicin (RIF) in the treatment of biofilm-embedded methicillin-resistant Staphylococcus aureus (MRSA). However, the development of RIF resistance in all combination therapies decreased their effectiveness over time.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Review
Clinical Neurology
Sara Otero Torres, Roser Juvany Roig, Merce Falip Centellas, Miriam Casellas Gibert, Monica Estopina Antoli, Rauel Rigo Bonnin, Jacint Xavier Sala-Padro, Ramon Jodar Massanes
Summary: This study aimed to evaluate the association between lacosamide plasma levels higher than 10 mg/L and the incidence of adverse effects. The results showed that lacosamide treatment within the range of 10 to 20 mg/L increases toxicity. It is suggested to adjust the upper limit of the reference range to 12 mg/L for a reasonable probability of efficacy and decreased toxicity.
CLINICAL NEUROPHARMACOLOGY
(2023)
Article
Genetics & Heredity
Daniela Matuozzo, Estelle Talouarn, Astrid Marchal, Peng Zhang, Jeremy Manry, Yoann Seeleuthner, Yu Zhang, Alexandre Bolze, Matthieu Chaldebas, Baptiste Milisavljevic, Adrian Gervais, Paul Bastard, Takaki Asano, Lucy Bizien, Federica Barzaghi, Hassan Abolhassani, Ahmad Abou Tayoun, Alessandro Aiuti, Ilad Alavi Darazam, Luis M. Allende, Rebeca Alonso-Arias, Andres Augusto Arias, Gokhan Aytekin, Peter Bergman, Simone Bondesan, Yenan T. Bryceson, Ingrid G. Bustos, Oscar Cabrera-Marante, Sheila Carcel, Paola Carrera, Giorgio Casari, Khalil Chaibi, Roger Colobran, Antonio Condino-Neto, Laura E. Covill, Ottavia M. Delmonte, Loubna El Zein, Carlos Flores, Peter K. Gregersen, Marta Gut, Filomeen Haerynck, Rabih Halwani, Selda Hancerli, Lennart Hammarstroem, Nevin Hatipoglu, Adem Karbuz, Sevgi Keles, Christele Kyheng, Rafael Leon-Lopez, Jose Luis Franco, Davood Mansouri, Javier Martinez-Picado, Ozge Metin Akcan, Isabelle Migeotte, Pierre-Emmanuel Morange, Guillaume Morelle, Andrea Martin-Nalda, Giuseppe Novelli, Antonio Novelli, Tayfun Ozcelik, Figen Palabiyik, Qiang Pan-Hammarstroem, Rebeca Perez de Diego, Laura Planas-Serra, Daniel E. Pleguezuelo, Carolina Prando, Aurora Pujol, Luis Felipe Reyes, Jacques G. Riviere, Carlos Rodriguez-Gallego, Julian Rojas, Patrizia Rovere-Querini, Agatha Schlueter, Mohammad Shahrooei, Ali Sobh, Pere Soler-Palacin, Yacine Tandjaoui-Lambiotte, Imran Tipu, Cristina Tresoldi, Jesus Troya, Diederik van de Beek, Mayana Zatz, Pawel Zawadzki, Saleh Zaid Al-Muhsen, Mohammed Faraj Alosaimi, Fahad M. Alsohime, Hagit Baris-Feldman, Manish J. Butte, Stefan N. Constantinescu, Megan A. Cooper, Clifton L. Dalgard, Jacques Fellay, James R. Heath, Yu-Lung Lau, Richard P. Lifton, Tom Maniatis, Trine H. Mogensen, Horst von Bernuth, Alban Lermine, Michel Vidaud, Anne Boland, Jean-Francois Deleuze, Robert Nussbaum, Amanda Kahn-Kirby, France Mentre, Sarah Tubiana, Guy Gorochov, Florence Tubach, Pierre Hausfater, C. O. V. I. D. Human Genetic Effort, Isabelle Meyts, Shen-Ying Zhang, Anne Puel, Luigi D. Notarangelo, Stephanie Boisson-Dupuis, Helen C. Su, Bertrand Boisson, Emmanuelle Jouanguy, Jean-Laurent Casanova, Qian Zhang, Laurent Abel, Aurelie Cobat
Summary: Through a genome-wide rare variant burden association analysis, it was found that there is an association between at-risk variants in the TLR7 gene and rare loss-of-function variants in TLR3-dependent type I interferon immunity genes. These findings suggest that rare variants in TLR3- and TLR7-dependent type I interferon immunity genes may underlie life-threatening COVID-19 in patients under 60 years old.
Article
Pharmacology & Pharmacy
Pere Fontova, Lisanne N. van Merendonk, Anna Vidal-Alabro, Raul Rigo-Bonnin, Gema Cerezo, Stefaan van Oevelen, Oriol Bestard, Edoardo Melilli, Nuria Montero, Ana Coloma, Anna Manonelles, Joan Torras, Josep M. Cruzado, Josep M. Grinyo, Helena Colom, Nuria Lloberas
Summary: Despite monitoring whole blood tacrolimus concentrations, acute rejection can still occur during tacrolimus therapy after kidney transplantation. This study aimed to investigate the intracellular pharmacokinetics (PK) of two tacrolimus formulations (immediate-release and extended-release) and their correlation with whole blood PK and pharmacodynamics (PD).
Article
Infectious Diseases
Javier Martinez-Casanova, Erika Esteve-Pitarch, Helena Colom-Codina, Victor Daniel Gumucio-Sanguino, Sara Cobo-Sacristan, Evelyn Shaw, Kristel Maisterra-Santos, Joan Sabater-Riera, Xose L. Perez-Fernandez, Raul Rigo-Bonnin, Fe Tubau-Quintano, Jordi Carratala, Ariadna Padulles-Zamora
Summary: This study aimed to characterize the pharmacokinetics of piperacillin after continuous infusion administration in critically ill adult patients and determine the optimal dosing regimen. The population pharmacokinetic approach was used to analyze piperacillin concentrations from 106 patients. Creatinine clearance was found to be the most predictive factor of piperacillin clearance. The model developed can be used for initial dose guidance and therapeutic drug monitoring.
Article
Infectious Diseases
Laura Rio-No, Luisa Sorli, Alba Arderiu-Formenti, Marta De Antonio, Lucas Martorell, Isaac Subirana, Lluis Puig, Albert Alier, Joan Gomez-Junyent, Daniel Perez-Prieto, Sonia Luque
Summary: This study aimed to investigate the pharmacokinetic/pharmacodynamic characteristics of vancomycin and identify risk factors associated with failure to achieve optimal PK/PD targets in the first therapeutic drug monitoring. The results showed that over 50% of patients did not achieve optimal PK/PD, and factors such as age, baseline serum creatinine, and body weight influenced drug exposure levels and the occurrence of nephrotoxicity.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Medical Laboratory Technology
Raul Rigo-Bonnin, Elena Rossello-Palmer, Arnau Sanchez-Garcia, Mireia Pallares-Borras, Yurema Martinez, Sebastian Videla, Aurema Otero, Nuria Lloberas, Miguel Hueso
Summary: The study developed and validated a UHPLC-MS/MS method for measuring apixaban concentrations in plasma, dialysate liquid, and urine. The results suggest that this method could support pharmacokinetic studies of apixaban-based anticoagulant therapy in chronic kidney disease patients undergoing hemodialysis.
CLINICA CHIMICA ACTA
(2023)
Article
Infectious Diseases
Eva Benavent, Marta Ulldemolins, Cristina El Haj, Raul Rigo-Bonnin, Heidi Yu, Lynn Wang, Hasini Wickremasinghe, Javier Ariza, Oscar Murillo
Summary: This study aimed to analyze the PK/PD indices of meropenem predicting anti-biofilm efficacy against meropenem-susceptible and meropenem-resistant strains of Pseudomonas aeruginosa. The results showed that f%T >MIC was the PK/PD index that best correlated with the anti-biofilm efficacy of meropenem. Using the extended infusion regimen of meropenem can better recover bactericidal activity in monotherapy, including activity against meropenem-resistant P. aeruginosa.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Daniel Podzamczer, Arkaitz Imaz, Ana Lopez-Lirola, Hernando Knobel, Mar Masia, Chiara Fanciulli, Cristina Hernandez, Maria Lagarde, Angela Gutierrez, Adria Curran, Luis Morano, Marta Montero-Alonso, Jesus Troya, Raul Rigo, Maria Casadella, Antonio Navarro-Alcaraz, Fernando Ardila, Mariona Parera, Enrique Bernal, Patricia Echeverria, Vicente Estrada, Carmen Hidalgo-Tenorio, Juan Macias, Paula Prieto, Joaquin Portilla, Eulalia Valencia, Maria Jesus Vivancos, Antonio Rivero
Summary: This study evaluated the efficacy and safety of a switching strategy using bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF) plus darunavir/cobicistat in heavily treatment-experienced people living with HIV. The results showed that this regimen was effective and well-tolerated, potentially improving convenience and long-term treatment success.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Urology & Nephrology
Nuria Lloberas, Josep M. Grinyo, Helena Colom, Anna Vidal-Alabro, Pere Fontova, Raul Rigo-Bonnin, Ariadna Padro, Oriol Bestard, Edoardo Melilli, Nuria Montero, Ana Coloma, Anna Manonelles, Maria Meneghini, Alex Fava, Joan Torras, Josep M. Cruzado
Summary: This study developed and validated a population pharmacokinetic model for tacrolimus dosing, which was found to be superior to traditional labeling-based dosing in achieving therapeutic targets. The model showed faster attainment of therapeutic levels and reduced dosage adjustments compared to the standard approach.
KIDNEY INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Sara Grillo, Miquel Pujol, Josep M. Miro, Joaquin Lopez-Contreras, Gorane Euba, Oriol Gasch, Lucia Boix-Palop, Maria Jose Garcia-Pais, Maria Teresa Perez-Rodriguez, Silvia Gomez-Zorrilla, Isabel Oriol, Luis Eduardo Lopez-Cortes, Maria Luisa Pedro-Botet, Rafael San-Juan, Jose Maria Aguado, Francesca Gioia, Simona Iftimie, Laura Morata, Alfredo Jover-Saenz, Graciano Garcia-Pardo, Belen Loeches, Alvaro Izquierdo-Cardenas, Ane Josune Goikoetxea, Aina Gomila-Grange, Beatriz Dietl, Damaris Berbel, Sebastian Videla, Pilar Hereu, Ariadna Padulles, Natalia Pallares, Cristian Tebe, Guillermo Cuervo, Jordi Carratala, SAFO Study Grp
Summary: In hospitalized adults with MSSA bacteremia, combining cloxacillin with fosfomycin did not achieve better treatment success at day 7 compared to using cloxacillin alone. Further trials should consider the heterogeneity of the infection and use a personalized approach.
Article
Infectious Diseases
Mar Ronda, Josep Manuel Llop-Talaveron, MariPaz Fuset, Elisabet Leiva, Evelyn Shaw, Victor Daniel Gumucio-Sanguino, Yolanda Diez, Helena Colom, Raul Rigo-Bonnin, Mireia Puig-Asensio, Jordi Carratala, Ariadna Padulles
Summary: This study compared the pharmacokinetics of voriconazole in critically ill patients with and without extracorporeal membrane oxygenation (ECMO) support. The results showed that the ECMO group had lower minimum plasma concentration (C-min) and a higher prevalence of subtherapeutic C-min values. ECMO support and plasma albumin levels were identified as risk factors for low C-min values. Drug optimization is crucial for achieving therapeutic C-min levels in the ECMO group.
Review
Medicine, Research & Experimental
Eduard Fort-Casamartina, Carme Munoz-Sanchez, Raul Francisco Rigo-Bonnin, Pamela Maria del Valle-Celiz, Nuria Gonzalo-Diego, Sara Otero-Torres, Carmen Bleda-Perez, Judith Prats-Jimenez, Sandra Fontanals-Martinez
Summary: This article discusses the importance of monitoring everolimus levels and potential drug interactions in patients on chronic medication. A case report of a 72-year-old female with renal cell carcinoma is presented, highlighting the need for therapeutic drug monitoring (TDM) due to drug interactions and potential underexposure to everolimus. Increasing the everolimus dose and regular monitoring resulted in improved drug levels.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Medical Laboratory Technology
Raul Rigo-Bonnin, Virginia Mas-Bosch, Francesca Canalias
Summary: This study presents a detailed estimation of measurement uncertainty (MU) for free drug concentrations using equilibrium dialysis (ED), considering different scenarios. The MU was estimated using a combination of top-down and bottom-up approaches based on ISO/TS 20914:2019 and JCGM 100:2008 guidelines. The expanded uncertainties ranged from 13.0% to 30.9%, with the highest MU observed when significant biases related to volume shift and Gibbs-Donnan effects exist.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)