Journal
INDUSTRIAL CROPS AND PRODUCTS
Volume 111, Issue -, Pages 579-589Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2017.11.015
Keywords
Medicinal plant; Subcritical water extraction; Antioxidant activity; Biosensor; Cytotoxic activity; HPLC-DAD analysis
Categories
Funding
- Portugal (FCT) [6818]
- Serbia (MSTD) [6818]
- Serbian Ministry of Education, Science and Technological Development [TR 31014]
- Republic of Serbia
- Republic of Croatia
- FCT/MEC
- FEDER [UID/QUI/50006/2013-POCI/01/0145/FEDER/007265]
- Fundacao para a Ciencia e Tecnologia [SFRH/BPD/78845/2011, SFRH/BPD/97049/2013]
- [NORTE-01-0145-FEDER-000011-311]
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Subcritical water extraction (SWE) has become a popular green extraction technique for the isolation of different classes of compounds from natural matrices. Low price, safety and green character of water, good yields of target compounds and reduced energy consumption, make this technique favorable for potential industrial applications. The purpose of this study was to evaluate antioxidant, antimicrobial and cytotoxic activity of four medicinal plants traditionally used in folk medicine of Serbia. Black mulberry (Morus nigra L.), wall germander (Teucriwn chamaedrys L.), wild geranium (Geranium macrorrhizwn L) and comfrey (Sytnphytum officinale L) were extracted by subcritical water at different temperatures. Antioxidant activity of the extracts was defined by conventional spectrophotometric methods, such as the total phenolic content (TPC), DPPH-radical scavenging activity (DPPH-RSA), ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) assessed by a DNA-based sensor. Additionally, the main phenolic compounds contributing to the antioxidant activity of the produced extracts were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). Antimicrobial properties of extracts were evaluated against eight microbial strains. Furthermore, the cytotoxic activity was observed for two human cancer cell lines and a cell line derived from murine fibroblast.
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