Article
Biotechnology & Applied Microbiology
Tinuola Olorunsogbon, Yinka Adesanya, Hasan K. Atiyeh, Christopher Chukwudi Okonkwo, Victor Chinomso Ujor, Thaddeus Chukwuemeka Ezeji
Summary: This study provides evidence on the effectiveness of metabolically engineered C. beijerinckii NCIMB 8052 for fermenting LB-derived hydrolysates to produce acetone-butanol-ethanol (ABE). The engineered strain showed higher butanol and ABE production in non-detoxified hydrolysates compared to the wildtype strain, and even higher production in detoxified hydrolysates.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Ibham Veza, Mohd Farid Muhamad Said, Zulkarnain Abdul Latiff
Summary: This review article discusses the history of the butanol industry and recent advances in butanol production by ABE fermentation, highlighting novel approaches such as searching for abundant and inexpensive substrates and optimizing processes. The challenges of biobutanol production, including low yield and end-product toxicity, as well as potential research routes, are summarized, along with the future research direction of butanol production by ABE fermentation.
BIOMASS & BIOENERGY
(2021)
Article
Biotechnology & Applied Microbiology
Li Liu, Yancui Wang, Na Wang, Xiaomiao Chen, Baoguo Li, Jiping Shi, Xiang Li
Summary: This study successfully improved the production efficiency of biobutanol by integrating pervaporation technology. It also discovered the unusual accumulation of ethanol during fed-batch fermentation, indicating the need for further investigation.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Review
Biotechnology & Applied Microbiology
Zhangnan Lin, Wei Cong, Jian'an Zhang
Summary: This article elaborates on the research progress of biobutanol fermentation, including microorganisms, metabolic pathways, raw materials, and technologies, and looks forward to the prospects of biobutanol.
FERMENTATION-BASEL
(2023)
Review
Engineering, Chemical
Bruno Jose Arcanjo Goncalves, Katia Cecilia de Souza Figueiredo
Summary: Biobutanol is a potential biofuel and industrial chemical, but its commercial application is limited by technical and economic constraints. Efforts have been made to improve the ABE fermentation process by separating butanol from the fermentation broth. This overview discusses the main separation processes reported in the literature, with a focus on pervaporation, and highlights the latest developments in the field.
CHEMICAL ENGINEERING & TECHNOLOGY
(2022)
Article
Engineering, Chemical
Hao-Yeh Lee, Tsung-Shi You, Cheng-Liang Chen
Summary: This article focuses on developing efficient separation sequences for purifying individual components from ABE fermentation, aiming to reduce energy consumption and achieve target purities. The proposed design alternatives, including the removal of acetone, integration of distillation and membrane for ethanol purification, heat integration, and column stacking, significantly reduce energy consumption and costs while maintaining target purities. Furthermore, a plantwide control scheme is validated for the integrated processes.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2022)
Article
Biotechnology & Applied Microbiology
Juan Carlos Quintero-Diaz, Diego F. Mendoza, Claudio Avignone-Rossa
Summary: In this study, a kinetic model of acetone-butanol-ethanol (ABE) fermentation based on enzyme kinetics expressions was proposed. The model takes into account the effect of the co-substrate NADH as a modulating factor of cellular metabolism. The simulations obtained with the model showed a good fit to experimental data, surpassing previous models. The introduction of NADH in the model offers a basis for analyzing electrofermentation processes with Clostridium.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Thermodynamics
Jianzheng Li, Yafei Zhang, Kai Sun, Wenbin Liu, Han Yan, Jia Meng
Summary: By optimizing the ABE fermentation process, this study successfully increased the co-production of butanol and H2, demonstrating an effective approach to manipulate microbial metabolism for enhanced production.
ENERGY CONVERSION AND MANAGEMENT
(2022)
Article
Engineering, Environmental
Javier Bravo-Venegas, Ines Prado-Acebo, Beatriz Gullon, Thelmo A. Lu-Chau, Gemma Eibes
Summary: Apple pomace (AP), a lignocellulosic residue, can be used to produce butanol by acetone-butanol-ethanol (ABE) fermentation. The prevention of acid crash occurrence in C. beijerinckii CECT 508 cultures was optimized by adjusting pH, CaCO3 concentration, and culture temperature. The optimal conditions of pH 7, 6.8 g/L CaCO3, and 30 degrees C resulted in a butanol production of 10.75 g/L. Additionally, the comparison between the supernatant of AP hydrolysate and the full hydrolysate indicated the possibility of eliminating the centrifugation step and achieving a zero-waste approach.
Article
Agricultural Engineering
Yinka Adesanya, Hasan K. Atiyeh, Tinuola Olorunsogbon, Asmita Khanal, Christopher C. Okonkwo, Victor C. Ujor, Ajay Shah, Thaddeus C. Ezeji
Summary: The study investigated a process engineering strategy to effectively convert hydrothermolysis pretreated non-detoxified switchgrass hydrolysates to acetone butanol ethanol (ABE) using a metabolically engineered strain of Clostridium beijerinckii. Through modifications to the engineered strain and the use of intermittent feeding, ABE fermentation was enhanced and detoxification of the hydrolysates prior to fermentation was eliminated. The ABE production from the non-detoxified hydrolysates by the engineered strain was comparable to the control medium, demonstrating the potential of this strategy in improving biofuel production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Marcelo Rivas-Astroza, Ivan Paredes, Karlo Guerrero, Silvia Mau, Julian Quintero, Juan Carlos Gentina, Raul Conejeros, German Aroca
Summary: A new kinetic model of Clostridium beijerinckii's Acetone-Butanol-Ethanol fermentation, considering three cell types, accurately replicates batch culture data. The model estimates cell type-specific kinetic parameters, which can improve the operation of ABE fermentation and provide a framework for studying acidogenic and solventogenic metabolic pathways.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Carlos Alberto Garcia Mogollon, Juan Carlos Quintero Diaz, Jorge Omar Gil Posada
Summary: This manuscript examines the impact of modifying the extracellular redox potential on the production of acetone, butanol, and ethanol in a microbial fuel cell. The addition of NADH enhances acetone production, while adjusting the cathode potential favors butanol production. The findings highlight the potential of bio-electrochemical systems to improve conventional fermentation processes.
BIOELECTROCHEMISTRY
(2023)
Article
Microbiology
Manish Kumar, Supreet Saini, Kalyan Gayen
Summary: We developed a pH-based phenomenological model to predict the fermentative production of butanol from glucose using C. acetobutylicum in a batch system. The model describes the relationship between the dynamics of growth and the production of desired metabolites and the extracellular pH of the media. Our model was successful in predicting the fermentation dynamics of C. acetobutylicum and can be extended to represent the dynamics of butanol production in other fermentation systems.
Article
Green & Sustainable Science & Technology
Samir Meramo, Arturo Gonzalez-Quiroga, Angel Gonzalez-Delgado
Summary: The North-Colombian region has great potential for producing bioproducts and bioenergy from agricultural residues. This study assesses two alternatives for transforming cassava residues via ABE fermentation and finds that both routes have hazards and differ in terms of environmental impacts and energy utilization.
Article
Biotechnology & Applied Microbiology
Miguel Capilla, Carlos Silvestre, Alejo Valles, Francisco Javier Alvarez-Hornos, Pau San-Valero, Carmen Gabaldon
Summary: The study showed that pH regulation can influence monosaccharide composition in batch and continuous fermentation processes, impacting butanol production and productivity.
FERMENTATION-BASEL
(2022)