Bio-inspired synthesis of sulphur nanoparticles using leaf extract of four medicinal plants with special reference to their antibacterial activity

We report new, eco-friendly and green method for the synthesis of sulphur nanoparticles using sodium polysulphide in the presence of leaf extracts of four different medicinal plants, which can be used for treatment of bacterial infections. Sodium polysulphide and acidic solution (H2SO4) in the presence of plant leaf extract developed the yellowish precipitate in solution, which indicated the formation of sulphur nanoparticles. UV-Vis spectrophotometer analysis of reaction mixture showed absorbance spectra in the range of 292-296nm, which is supposed to be specific for sulphur nanoparticles. Zeta potential study of sulphur nanoparticles synthesized from Catharanthus roseus showed more stability when compared with other medicinal plants. Sulphur nanoparticles synthesized from C. roseus were further characterized by XRD analysis, FTIR analysis, and TEM analysis. The biogenic sulphur nanoparticles were spherical, polydispersed with particle size of 70-80nm. Evaluation of antibacterial study revealed that synthesized sulphur nanoparticles exhibited better bactericidal efficacy against common pathogenic bacteria Escherichia coli and Staphylococcus aureus with minimum inhibitory concentration of 200g/ml with significant activity used in combination with antibiotic. It can be concluded that the synthesized sulphur nanoparticles can be used as antibacterial agents after thorough experimental trials in animals.