Journal
GENETICS AND MOLECULAR RESEARCH
Volume 17, Issue 2, Pages -Publisher
FUNPEC-EDITORA
DOI: 10.4238/gmr16039916
Keywords
hydroxybenzoate 3-monooxygenase (pobA); Ketoacyl-ACP synthase III (fabHB); Bacillus licheniformis M2-7; benzo[a]pyrene; Reverse transcription quantitative (PCR)
Funding
- Consejo Nacional de Ciencia y Tecnologia [597152]
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Bacillus licheniformis M2-7 is a heat-resistant bacterium able to biotransform polycyclic aromatic hydrocarbons. It can transform a wide range of these compounds as naphthalene, phenanthrene, pyrene and benzo[a]pyrene. Benzo[a]pyrene is a polycyclic aromatic hydrocarbon of high molecular weight considered as potentially toxic and carcinogenic for humans. Aiming to discover the genes involved in the biotransformation of benzo[a]pyrene, we made a B. licheniformis M2-7 genomic library in E. coli. We isolated two E. coli strains that were able to grow in minimal salt medium supplemented with benzo[a]pyrene. From the analysis of the DNA fragments in the clones H23 and H38, we identified open reading frames coding for 5 possible genes, among them pobA and fabHB, which products are the enzymes 4-hydroxybenzoate 3-monooxygenase and the ketoacyl-ACP synthase III, respectively. To evaluate the role of these genes in the metabolism of benzo[a]pyrene in B. licheniformis M2-7, we estimated their relative expression through reverse transcription quantitative PCR. Finally, we observed that the genes pobA and fabHB were overexpressed after 3 h under induction with benzo[a]pyrene, suggesting that this strain could use these genes during the metabolism of this PAH, plus it does it in a faster time than that reported for other bacterial genera
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