Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus

Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes

(2017) Ashley M. Jacobi et al.   METHODS

Unexpected mutations after CRISPR–Cas9 editing in vivo

(2017) Kellie A Schaefer et al.   NATURE METHODS

Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system

(2017) Kazuo Takayama et al.   NUCLEIC ACIDS RESEARCH

Promoting Cas9 degradation reduces mosaic mutations in non-human primate embryos

(2017) Zhuchi Tu et al.   Scientific Reports

Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX

(2016) Xin Yu et al.   BIOTECHNOLOGY LETTERS

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA

(2016) Christopher D Richardson et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice

(2016) Asami Oji et al.   Scientific Reports

Mammalian Reverse Genetics without Crossing Reveals Nr3a as a Short-Sleeper Gene

(2016) Genshiro A. Sunagawa et al.   Cell Reports

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

(2015) Tetsushi Sakuma et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection

(2015) Xiquan Liang et al.   JOURNAL OF BIOTECHNOLOGY

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

(2015) Kathrin Schumann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

(2015) Tomomi Aida et al.   GENOME BIOLOGY

Screening Methods to Identify TALEN-Mediated Knockout Mice

(2014) Yoshiko Nakagawa et al.   EXPERIMENTAL ANIMALS

Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing

(2014) Yoshiko Nakagawa et al.   EXPERIMENTAL ANIMALS

FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions

(2014) Daisuke Tokumasu et al.   GENES TO CELLS

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

(2014) S. Ramakrishna et al.   GENOME RESEARCH

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

(2014) John A Zuris et al.   NATURE BIOTECHNOLOGY

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9

(2014) Shota Nakade et al.   Nature Communications

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

(2014) Tetsushi Sakuma et al.   Scientific Reports

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

(2014) Steven Lin et al.   eLife

COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites

(2014) Thomas J Cradick et al.   Molecular Therapy-Nucleic Acids

High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos

(2013) K.-i. T. Suzuki et al.   Biology Open

A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models

(2012) Qiurong Ding et al.   Cell Stem Cell

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE