Journal
GENE
Volume 659, Issue -, Pages 11-21Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2018.03.046
Keywords
Cloning; Functional characterization; elovl5; Nibea coibor; Nutritional study; LC-PUFA
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Funding
- National Natural Science Foundation of China [31172424]
- Guangdong Oceanic and Fishery Science and Technology Foundation [A201005D06-1]
- Young Creative Talents, Major Scientific Research Projects of Guangdong University Foundation [2016KQNCX058]
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Enzymes that lengthen the carbon chain of polyunsaturated fatty acids (PUFA) are key to the biosynthesis of the long-chain polyunsaturated fatty acids (LC-PUFA). Here we report on the molecular cloning, tissue distribution, functional characterization and nutritional regulation of a elovl5 gene from Nibea coibor. The full-length cDNA was 1315 bp, including a 5-untranslated region (UTR) of 134 bp, a 3-UTR of 296 bp and an open reading frame of 885 bp, which specified a peptide of 294 amino acids. Bioinformatics analysis showed that the deduced peptide sequence possessed all the characteristic features of microsomal fatty acyl elongases, including the so-called histidine box (HXXHH), the canonical C-terminal endoplasmic reticulum retention signal, several predicted transmembrane regions and other highly conserved motifs. Expression of elovl5 was strongly observed in stomach, and more weakly in kidney, spleen, intestine, brain, eye, liver, gill, muscle and heart. Functional characterization revealed that the chu's croaker Elovl5 was able to elongate both C18 and C20 PUFA substrates. Nutritional study indicated that the hepatic expression of elovl5 could be up-regulated by low dietary n - 3 LC-PUFA. These results may contribute to better understanding the LC-PUFA biosynthetic pathway and regulation mechanism in chu's croaker.
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